Difference in intracellular temperature rise between matured and precursor brown adipocytes in response to uncoupler and β-adrenergic agonist stimuli
Abstract Brown adipocytes function to maintain body temperature by heat production. However, direct measurement of heat production at a single cell level remains difficult. Here we developed a method to measure the temperature within primary cultured brown adipocytes using a cationic fluorescent pol...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2017-10-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-12634-7 |
| _version_ | 1818991418297810944 |
|---|---|
| author | Toshikazu Tsuji Kumiko Ikado Hideki Koizumi Seiichi Uchiyama Kazuaki Kajimoto |
| author_facet | Toshikazu Tsuji Kumiko Ikado Hideki Koizumi Seiichi Uchiyama Kazuaki Kajimoto |
| author_sort | Toshikazu Tsuji |
| collection | DOAJ |
| description | Abstract Brown adipocytes function to maintain body temperature by heat production. However, direct measurement of heat production at a single cell level remains difficult. Here we developed a method to measure the temperature within primary cultured brown adipocytes using a cationic fluorescent polymeric thermometer. Placement of the thermometer within a matured brown adipocyte and a precursor cell enabled the detection of heat production following uncoupler treatment. The increase in the intracellular temperature due to stimulation with a mitochondrial uncoupler was higher in matured brown adipocytes than in precursor cells. Stimulation with a β-adrenergic receptor (β-AR) agonist, norepinephrine, raised the intracellular temperature of matured brown adipocytes to a level comparable to that observed after stimulation with a β3-AR-specific agonist, CL316.243. In contrast, neither β-AR agonist induced an intracellular temperature increase in precursor cells. Further, pretreatment of brown adipocytes with a β3-AR antagonist inhibited the norepinephrine-stimulated elevation of temperature. These results demonstrate that our novel method successfully determined the difference in intracellular temperature increase between matured brown adipocytes and precursor cells in response to stimulation by an uncoupler and β-AR agonists. |
| first_indexed | 2024-12-20T20:09:57Z |
| format | Article |
| id | doaj.art-57581b582d5d4c79a092d33e9546b322 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-20T20:09:57Z |
| publishDate | 2017-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-57581b582d5d4c79a092d33e9546b3222022-12-21T19:27:51ZengNature PortfolioScientific Reports2045-23222017-10-017111310.1038/s41598-017-12634-7Difference in intracellular temperature rise between matured and precursor brown adipocytes in response to uncoupler and β-adrenergic agonist stimuliToshikazu Tsuji0Kumiko Ikado1Hideki Koizumi2Seiichi Uchiyama3Kazuaki Kajimoto4Central Laboratories for Key Technologies, KIRIN Company Limited, 1-13-5 Fukuura, Kanazawa-kuCentral Laboratories for Key Technologies, KIRIN Company Limited, 1-13-5 Fukuura, Kanazawa-kuCentral Laboratories for Key Technologies, KIRIN Company Limited, 1-13-5 Fukuura, Kanazawa-kuGraduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 HongoFaculty of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-kuAbstract Brown adipocytes function to maintain body temperature by heat production. However, direct measurement of heat production at a single cell level remains difficult. Here we developed a method to measure the temperature within primary cultured brown adipocytes using a cationic fluorescent polymeric thermometer. Placement of the thermometer within a matured brown adipocyte and a precursor cell enabled the detection of heat production following uncoupler treatment. The increase in the intracellular temperature due to stimulation with a mitochondrial uncoupler was higher in matured brown adipocytes than in precursor cells. Stimulation with a β-adrenergic receptor (β-AR) agonist, norepinephrine, raised the intracellular temperature of matured brown adipocytes to a level comparable to that observed after stimulation with a β3-AR-specific agonist, CL316.243. In contrast, neither β-AR agonist induced an intracellular temperature increase in precursor cells. Further, pretreatment of brown adipocytes with a β3-AR antagonist inhibited the norepinephrine-stimulated elevation of temperature. These results demonstrate that our novel method successfully determined the difference in intracellular temperature increase between matured brown adipocytes and precursor cells in response to stimulation by an uncoupler and β-AR agonists.https://doi.org/10.1038/s41598-017-12634-7 |
| spellingShingle | Toshikazu Tsuji Kumiko Ikado Hideki Koizumi Seiichi Uchiyama Kazuaki Kajimoto Difference in intracellular temperature rise between matured and precursor brown adipocytes in response to uncoupler and β-adrenergic agonist stimuli Scientific Reports |
| title | Difference in intracellular temperature rise between matured and precursor brown adipocytes in response to uncoupler and β-adrenergic agonist stimuli |
| title_full | Difference in intracellular temperature rise between matured and precursor brown adipocytes in response to uncoupler and β-adrenergic agonist stimuli |
| title_fullStr | Difference in intracellular temperature rise between matured and precursor brown adipocytes in response to uncoupler and β-adrenergic agonist stimuli |
| title_full_unstemmed | Difference in intracellular temperature rise between matured and precursor brown adipocytes in response to uncoupler and β-adrenergic agonist stimuli |
| title_short | Difference in intracellular temperature rise between matured and precursor brown adipocytes in response to uncoupler and β-adrenergic agonist stimuli |
| title_sort | difference in intracellular temperature rise between matured and precursor brown adipocytes in response to uncoupler and β adrenergic agonist stimuli |
| url | https://doi.org/10.1038/s41598-017-12634-7 |
| work_keys_str_mv | AT toshikazutsuji differenceinintracellulartemperaturerisebetweenmaturedandprecursorbrownadipocytesinresponsetouncouplerandbadrenergicagoniststimuli AT kumikoikado differenceinintracellulartemperaturerisebetweenmaturedandprecursorbrownadipocytesinresponsetouncouplerandbadrenergicagoniststimuli AT hidekikoizumi differenceinintracellulartemperaturerisebetweenmaturedandprecursorbrownadipocytesinresponsetouncouplerandbadrenergicagoniststimuli AT seiichiuchiyama differenceinintracellulartemperaturerisebetweenmaturedandprecursorbrownadipocytesinresponsetouncouplerandbadrenergicagoniststimuli AT kazuakikajimoto differenceinintracellulartemperaturerisebetweenmaturedandprecursorbrownadipocytesinresponsetouncouplerandbadrenergicagoniststimuli |