Linearly modulated multi-focal diffractive lens for multi-sheet excitation of flow driven samples in a light-sheet fluorescence microscope
Light sheet fluorescence microscope with single light sheet illumination enables rapid 3D imaging of living cells. In this paper we show the design, fabrication and characterization of a diffractive optical element producing several light sheets along a 45° inclined tube. The element, which is based...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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EDP Sciences
2023-01-01
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| Series: | Journal of the European Optical Society-Rapid Publications |
| Subjects: | |
| Online Access: | https://jeos.edpsciences.org/articles/jeos/full_html/2023/01/jeos20230017/jeos20230017.html |
| _version_ | 1797807641036062720 |
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| author | Hofmann Meike Gharbi Ghebjagh Shima Feng Yuchao Fan Chao Lemke Karen Sinzinger Stefan |
| author_facet | Hofmann Meike Gharbi Ghebjagh Shima Feng Yuchao Fan Chao Lemke Karen Sinzinger Stefan |
| author_sort | Hofmann Meike |
| collection | DOAJ |
| description | Light sheet fluorescence microscope with single light sheet illumination enables rapid 3D imaging of living cells. In this paper we show the design, fabrication and characterization of a diffractive optical element producing several light sheets along a 45° inclined tube. The element, which is based on a multi-focal diffractive lens and a linear grating, generates five thin light sheets with equal intensities when combined with a refractive cylindrical lens. The generated uniform light sheets can be applied for the scanning of samples in tubes enabling flow-driven 3-dimensional imaging. |
| first_indexed | 2024-03-13T06:25:41Z |
| format | Article |
| id | doaj.art-5759d230024e4eac969bf2d55ed385d2 |
| institution | Directory Open Access Journal |
| issn | 1990-2573 |
| language | English |
| last_indexed | 2024-03-13T06:25:41Z |
| publishDate | 2023-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | Journal of the European Optical Society-Rapid Publications |
| spelling | doaj.art-5759d230024e4eac969bf2d55ed385d22023-06-09T09:24:37ZengEDP SciencesJournal of the European Optical Society-Rapid Publications1990-25732023-01-011912610.1051/jeos/2023022jeos20230017Linearly modulated multi-focal diffractive lens for multi-sheet excitation of flow driven samples in a light-sheet fluorescence microscopeHofmann Meike0https://orcid.org/0000-0002-0448-2479Gharbi Ghebjagh Shima1https://orcid.org/0000-0002-0648-4670Feng Yuchao2Fan Chao3Lemke Karen4Sinzinger Stefan5Technische Universität Ilmenau, Fachgebiet Technische OptikTechnische Universität Ilmenau, Fachgebiet Technische OptikTechnische Universität Ilmenau, Fachgebiet Technische OptikTechnische Universität Ilmenau, Fachgebiet Technische OptikIba HeiligenstadtTechnische Universität Ilmenau, Fachgebiet Technische OptikLight sheet fluorescence microscope with single light sheet illumination enables rapid 3D imaging of living cells. In this paper we show the design, fabrication and characterization of a diffractive optical element producing several light sheets along a 45° inclined tube. The element, which is based on a multi-focal diffractive lens and a linear grating, generates five thin light sheets with equal intensities when combined with a refractive cylindrical lens. The generated uniform light sheets can be applied for the scanning of samples in tubes enabling flow-driven 3-dimensional imaging.https://jeos.edpsciences.org/articles/jeos/full_html/2023/01/jeos20230017/jeos20230017.htmllight sheet fluorescence microscopydiffractive optical elementsmulti-plane imaging3d imagingmulti-focal lens |
| spellingShingle | Hofmann Meike Gharbi Ghebjagh Shima Feng Yuchao Fan Chao Lemke Karen Sinzinger Stefan Linearly modulated multi-focal diffractive lens for multi-sheet excitation of flow driven samples in a light-sheet fluorescence microscope Journal of the European Optical Society-Rapid Publications light sheet fluorescence microscopy diffractive optical elements multi-plane imaging 3d imaging multi-focal lens |
| title | Linearly modulated multi-focal diffractive lens for multi-sheet excitation of flow driven samples in a light-sheet fluorescence microscope |
| title_full | Linearly modulated multi-focal diffractive lens for multi-sheet excitation of flow driven samples in a light-sheet fluorescence microscope |
| title_fullStr | Linearly modulated multi-focal diffractive lens for multi-sheet excitation of flow driven samples in a light-sheet fluorescence microscope |
| title_full_unstemmed | Linearly modulated multi-focal diffractive lens for multi-sheet excitation of flow driven samples in a light-sheet fluorescence microscope |
| title_short | Linearly modulated multi-focal diffractive lens for multi-sheet excitation of flow driven samples in a light-sheet fluorescence microscope |
| title_sort | linearly modulated multi focal diffractive lens for multi sheet excitation of flow driven samples in a light sheet fluorescence microscope |
| topic | light sheet fluorescence microscopy diffractive optical elements multi-plane imaging 3d imaging multi-focal lens |
| url | https://jeos.edpsciences.org/articles/jeos/full_html/2023/01/jeos20230017/jeos20230017.html |
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