Identification and Expression Profile of CYPome in Perennial Ryegrass and Tall Fescue in Response to Temperature Stress
Plant cytochrome P450s are involved in a wide range of biosynthetic reactions that generate various biomolecules, including a variety of defensive compounds. Perennial ryegrass (Lolium perenne) and tall fescue (Festuca arundinacea) are two major species of turf and forage grasses that usually experi...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-11-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2017.01519/full |
| _version_ | 1818893514578067456 |
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| author | Xiang Tao Ming-Xiu Wang Ming-Xiu Wang Ya Dai Ya Dai Yan Wang Yan-Fen Fan Yan-Fen Fan Ping Mao Xin-Rong Ma |
| author_facet | Xiang Tao Ming-Xiu Wang Ming-Xiu Wang Ya Dai Ya Dai Yan Wang Yan-Fen Fan Yan-Fen Fan Ping Mao Xin-Rong Ma |
| author_sort | Xiang Tao |
| collection | DOAJ |
| description | Plant cytochrome P450s are involved in a wide range of biosynthetic reactions that generate various biomolecules, including a variety of defensive compounds. Perennial ryegrass (Lolium perenne) and tall fescue (Festuca arundinacea) are two major species of turf and forage grasses that usually experience low temperature below −10°C and high temperature over 38°C around the world. In this study, we re-analyzed transcriptome of perennial ryegrass and tall fescue treated with heat and cold stress. Thus, we can evaluate P450 composition in these species and confirm whether P450 genes response to temperature stress. We identified 277 and 319 P450 transcripts with open reading frames larger than 300 bp, respectively. These P450 transcripts were mainly classed in the CYP71, 51, 94, 89, 72, and 734 families. In perennial ryegrass and tall fescue, a total of 66 and 62 P450 transcripts were up-regulated, and 65 and 117 transcripts were down-regulated when subjected to heat stress, respectively. When exposed to cold stress, 60 and 73 transcripts were up-regulated, and 59 and 77 transcripts were down-regulated in perennial ryegrass and tall fescue. Among these differentially expressed transcripts, 64 and 87 of them showed expression level changes that followed the same trend, and these temperature-responsive genes primarily belong to the CYP71, 72 and 99 families. Besides, heat and cold stress altered phenylalanine and brassinosteroid involved P450 transcripts in perennial ryegrass and tall fescue. P450 transcripts involved in the metabolism of these compounds showed a strong response to heat and/or cold stress, indicating that they likely play important roles in temperature acclimation in these two species. The CYPome provide a genetic base for the future functional studies, as well as genetic studies that may improve stress tolerance for perennial ryegrass and tall fescue to extreme temperature. |
| first_indexed | 2024-12-19T18:13:49Z |
| format | Article |
| id | doaj.art-575d5f5debfc4af1ad22ba2370c1c539 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-19T18:13:49Z |
| publishDate | 2017-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-575d5f5debfc4af1ad22ba2370c1c5392022-12-21T20:11:12ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-11-01810.3389/fpls.2017.01519265164Identification and Expression Profile of CYPome in Perennial Ryegrass and Tall Fescue in Response to Temperature StressXiang Tao0Ming-Xiu Wang1Ming-Xiu Wang2Ya Dai3Ya Dai4Yan Wang5Yan-Fen Fan6Yan-Fen Fan7Ping Mao8Xin-Rong Ma9Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, ChinaChengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, ChinaCollege of Life Sciences, Sichuan University, Chengdu, ChinaChengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaChengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, ChinaChengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaChengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, ChinaChengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, ChinaPlant cytochrome P450s are involved in a wide range of biosynthetic reactions that generate various biomolecules, including a variety of defensive compounds. Perennial ryegrass (Lolium perenne) and tall fescue (Festuca arundinacea) are two major species of turf and forage grasses that usually experience low temperature below −10°C and high temperature over 38°C around the world. In this study, we re-analyzed transcriptome of perennial ryegrass and tall fescue treated with heat and cold stress. Thus, we can evaluate P450 composition in these species and confirm whether P450 genes response to temperature stress. We identified 277 and 319 P450 transcripts with open reading frames larger than 300 bp, respectively. These P450 transcripts were mainly classed in the CYP71, 51, 94, 89, 72, and 734 families. In perennial ryegrass and tall fescue, a total of 66 and 62 P450 transcripts were up-regulated, and 65 and 117 transcripts were down-regulated when subjected to heat stress, respectively. When exposed to cold stress, 60 and 73 transcripts were up-regulated, and 59 and 77 transcripts were down-regulated in perennial ryegrass and tall fescue. Among these differentially expressed transcripts, 64 and 87 of them showed expression level changes that followed the same trend, and these temperature-responsive genes primarily belong to the CYP71, 72 and 99 families. Besides, heat and cold stress altered phenylalanine and brassinosteroid involved P450 transcripts in perennial ryegrass and tall fescue. P450 transcripts involved in the metabolism of these compounds showed a strong response to heat and/or cold stress, indicating that they likely play important roles in temperature acclimation in these two species. The CYPome provide a genetic base for the future functional studies, as well as genetic studies that may improve stress tolerance for perennial ryegrass and tall fescue to extreme temperature.http://journal.frontiersin.org/article/10.3389/fpls.2017.01519/fullCYPomecoldheattranscriptomeperennial ryegrasstall fescue |
| spellingShingle | Xiang Tao Ming-Xiu Wang Ming-Xiu Wang Ya Dai Ya Dai Yan Wang Yan-Fen Fan Yan-Fen Fan Ping Mao Xin-Rong Ma Identification and Expression Profile of CYPome in Perennial Ryegrass and Tall Fescue in Response to Temperature Stress Frontiers in Plant Science CYPome cold heat transcriptome perennial ryegrass tall fescue |
| title | Identification and Expression Profile of CYPome in Perennial Ryegrass and Tall Fescue in Response to Temperature Stress |
| title_full | Identification and Expression Profile of CYPome in Perennial Ryegrass and Tall Fescue in Response to Temperature Stress |
| title_fullStr | Identification and Expression Profile of CYPome in Perennial Ryegrass and Tall Fescue in Response to Temperature Stress |
| title_full_unstemmed | Identification and Expression Profile of CYPome in Perennial Ryegrass and Tall Fescue in Response to Temperature Stress |
| title_short | Identification and Expression Profile of CYPome in Perennial Ryegrass and Tall Fescue in Response to Temperature Stress |
| title_sort | identification and expression profile of cypome in perennial ryegrass and tall fescue in response to temperature stress |
| topic | CYPome cold heat transcriptome perennial ryegrass tall fescue |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2017.01519/full |
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