Cellular <sup>1</sup>H MR Relaxation Times in Healthy and Cancer Three-Dimensional (3D) Breast Cell Culture
Noninvasive measurements of <sup>1</sup>H Magnetic Resonance Imaging (MR) relaxation times in a three-dimensional (3D) cell culture construct are presented. Trastuzumab was used as a pharmacological component delivered to the cells in vitro. The purpose of this study was to evaluate the...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/5/4735 |
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| author | Zuzanna Bober Rafał Podgórski David Aebisher Grzegorz Cieślar Aleksandra Kawczyk-Krupka Dorota Bartusik-Aebisher |
| author_facet | Zuzanna Bober Rafał Podgórski David Aebisher Grzegorz Cieślar Aleksandra Kawczyk-Krupka Dorota Bartusik-Aebisher |
| author_sort | Zuzanna Bober |
| collection | DOAJ |
| description | Noninvasive measurements of <sup>1</sup>H Magnetic Resonance Imaging (MR) relaxation times in a three-dimensional (3D) cell culture construct are presented. Trastuzumab was used as a pharmacological component delivered to the cells in vitro. The purpose of this study was to evaluate the Trastuzumab delivery by relaxation times in 3D cell cultures. The bioreactor has been designed and used for 3D cell cultures. Four bioreactors were prepared, two with normal cells and two with breast cancer cells. The relaxation times of HTB-125 and CRL 2314 cell cultures were determined. An immunohistochemistry (IHC) test was performed before MRI measurements to confirm the amount of HER2 protein in the CRL-2314 cancer cells. The results showed that the relaxation time of CRL2314 cells is lower than normal HTB-125 cells in both cases, before and after treatment. An analysis of the results showed that 3D culture studies have potential in evaluating treatment efficacy using relaxation times measurements with a field of 1.5 Tesla. The use <sup>1</sup>H MRI relaxation times allows for the visualization of cell viability in response to treatment. |
| first_indexed | 2024-03-11T07:22:31Z |
| format | Article |
| id | doaj.art-57655097005043f68e8e40ccf6723e86 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T07:22:31Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-57655097005043f68e8e40ccf6723e862023-11-17T07:52:39ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-03-01245473510.3390/ijms24054735Cellular <sup>1</sup>H MR Relaxation Times in Healthy and Cancer Three-Dimensional (3D) Breast Cell CultureZuzanna Bober0Rafał Podgórski1David Aebisher2Grzegorz Cieślar3Aleksandra Kawczyk-Krupka4Dorota Bartusik-Aebisher5Department of Photomedicine and Physical Chemistry, Medical College, Rzeszów University, 35-310 Rzeszów, PolandDepartment of Biochemistry and General Chemistry, Medical College, Rzeszów University, 35-310 Rzeszów, PolandDepartment of Photomedicine and Physical Chemistry, Medical College, Rzeszów University, 35-310 Rzeszów, PolandDepartment of Internal Medicine, Angiology and Physical Medicine, Center for Laser Diagnostics and Therapy, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 40-055 Katowice, PolandDepartment of Internal Medicine, Angiology and Physical Medicine, Center for Laser Diagnostics and Therapy, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 40-055 Katowice, PolandDepartment of Biochemistry and General Chemistry, Medical College, Rzeszów University, 35-310 Rzeszów, PolandNoninvasive measurements of <sup>1</sup>H Magnetic Resonance Imaging (MR) relaxation times in a three-dimensional (3D) cell culture construct are presented. Trastuzumab was used as a pharmacological component delivered to the cells in vitro. The purpose of this study was to evaluate the Trastuzumab delivery by relaxation times in 3D cell cultures. The bioreactor has been designed and used for 3D cell cultures. Four bioreactors were prepared, two with normal cells and two with breast cancer cells. The relaxation times of HTB-125 and CRL 2314 cell cultures were determined. An immunohistochemistry (IHC) test was performed before MRI measurements to confirm the amount of HER2 protein in the CRL-2314 cancer cells. The results showed that the relaxation time of CRL2314 cells is lower than normal HTB-125 cells in both cases, before and after treatment. An analysis of the results showed that 3D culture studies have potential in evaluating treatment efficacy using relaxation times measurements with a field of 1.5 Tesla. The use <sup>1</sup>H MRI relaxation times allows for the visualization of cell viability in response to treatment.https://www.mdpi.com/1422-0067/24/5/4735cellular MRIbreast cancerviability1.5 Tesla |
| spellingShingle | Zuzanna Bober Rafał Podgórski David Aebisher Grzegorz Cieślar Aleksandra Kawczyk-Krupka Dorota Bartusik-Aebisher Cellular <sup>1</sup>H MR Relaxation Times in Healthy and Cancer Three-Dimensional (3D) Breast Cell Culture International Journal of Molecular Sciences cellular MRI breast cancer viability 1.5 Tesla |
| title | Cellular <sup>1</sup>H MR Relaxation Times in Healthy and Cancer Three-Dimensional (3D) Breast Cell Culture |
| title_full | Cellular <sup>1</sup>H MR Relaxation Times in Healthy and Cancer Three-Dimensional (3D) Breast Cell Culture |
| title_fullStr | Cellular <sup>1</sup>H MR Relaxation Times in Healthy and Cancer Three-Dimensional (3D) Breast Cell Culture |
| title_full_unstemmed | Cellular <sup>1</sup>H MR Relaxation Times in Healthy and Cancer Three-Dimensional (3D) Breast Cell Culture |
| title_short | Cellular <sup>1</sup>H MR Relaxation Times in Healthy and Cancer Three-Dimensional (3D) Breast Cell Culture |
| title_sort | cellular sup 1 sup h mr relaxation times in healthy and cancer three dimensional 3d breast cell culture |
| topic | cellular MRI breast cancer viability 1.5 Tesla |
| url | https://www.mdpi.com/1422-0067/24/5/4735 |
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