Multiple Plant Regeneration from Embryogenic Calli of <i>Paulownia tomentosa</i> (Thunb.) Steud
The aim of this paper was to study the effect of plant growth regulators on callus induction and <i>in vitro</i> morphogenesis using various explants of <i>Paulownia tomentosa</i> to develop an efficient plant regeneration protocol. Different plant organ sections (leaves, api...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-04-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/11/8/1020 |
| _version_ | 1797444160684294144 |
|---|---|
| author | Aigul Amirova Symbat Dossymbetova Yeldana Rysbayeva Bakdaulet Usenbekov Arman Tolegen Alibek Ydyrys |
| author_facet | Aigul Amirova Symbat Dossymbetova Yeldana Rysbayeva Bakdaulet Usenbekov Arman Tolegen Alibek Ydyrys |
| author_sort | Aigul Amirova |
| collection | DOAJ |
| description | The aim of this paper was to study the effect of plant growth regulators on callus induction and <i>in vitro</i> morphogenesis using various explants of <i>Paulownia tomentosa</i> to develop an efficient plant regeneration protocol. Different plant organ sections (leaves, apical shoot tips, petals, nodes, and internodes) were cultured as explants to identify the best <i>in vitro</i> explants responsive to callus induction and plant regeneration. Explants were cultivated on MS media supplemented with different concentrations of plant growth regulators (TDZ (Thidiazuron), BAP (6-Benzylaminopurine), kinetin, and NAA (1-Naphthaleneacetic acid). It was discovered that the addition of TDZ and NAA stimulated the induction of somatic embryogenesis. It was discovered that the MS medium with the combination of plant growth regulators BAP (35.5 µM) and NAA (5.4 µM) with the addition of 30.0 g/L maltose, 500.0 mg/L casein hydrolysate, and 250.0 mg/L L-proline was optimal for callus induction and multiple plant regeneration. The study of the regenerative capacity of various explants of <i>Paulownia tomentosa in vitro</i> showed that plant regeneration depends on the type of explant, and occurs in both ways, indirectly, through the formation of callus tissues and directly on the explant, without callus formation. As a result of this study, the efficient reproducible protocol of embryogenic callus formation and multiple shoot induction <i>in vitro</i> of <i>Paulownia tomentosa</i> was developed. This system provides a clear increase in the frequency of plant regeneration from 36.3 ± 3.4% to 38.6 ± 2.3% per embryogenic callus from leaves and apical shoot tips, respectively. |
| first_indexed | 2024-03-09T13:07:33Z |
| format | Article |
| id | doaj.art-5766f9e403b344419b88bd6f7ff481b6 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T13:07:33Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-5766f9e403b344419b88bd6f7ff481b62023-11-30T21:46:13ZengMDPI AGPlants2223-77472022-04-01118102010.3390/plants11081020Multiple Plant Regeneration from Embryogenic Calli of <i>Paulownia tomentosa</i> (Thunb.) SteudAigul Amirova0Symbat Dossymbetova1Yeldana Rysbayeva2Bakdaulet Usenbekov3Arman Tolegen4Alibek Ydyrys5Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, al-Farabi Av. 71, Almaty 050040, KazakhstanDepartment of Food Technology, Almaty Technological University, Tole be 100, Almaty 050008, KazakhstanDepartment of Food Technology, Almaty Technological University, Tole be 100, Almaty 050008, KazakhstanFaculty of Biology and Biotechnology, Al-Farabi Kazakh National University, al-Farabi Av. 71, Almaty 050040, KazakhstanFaculty of Biology and Biotechnology, Al-Farabi Kazakh National University, al-Farabi Av. 71, Almaty 050040, KazakhstanFaculty of Biology and Biotechnology, Al-Farabi Kazakh National University, al-Farabi Av. 71, Almaty 050040, KazakhstanThe aim of this paper was to study the effect of plant growth regulators on callus induction and <i>in vitro</i> morphogenesis using various explants of <i>Paulownia tomentosa</i> to develop an efficient plant regeneration protocol. Different plant organ sections (leaves, apical shoot tips, petals, nodes, and internodes) were cultured as explants to identify the best <i>in vitro</i> explants responsive to callus induction and plant regeneration. Explants were cultivated on MS media supplemented with different concentrations of plant growth regulators (TDZ (Thidiazuron), BAP (6-Benzylaminopurine), kinetin, and NAA (1-Naphthaleneacetic acid). It was discovered that the addition of TDZ and NAA stimulated the induction of somatic embryogenesis. It was discovered that the MS medium with the combination of plant growth regulators BAP (35.5 µM) and NAA (5.4 µM) with the addition of 30.0 g/L maltose, 500.0 mg/L casein hydrolysate, and 250.0 mg/L L-proline was optimal for callus induction and multiple plant regeneration. The study of the regenerative capacity of various explants of <i>Paulownia tomentosa in vitro</i> showed that plant regeneration depends on the type of explant, and occurs in both ways, indirectly, through the formation of callus tissues and directly on the explant, without callus formation. As a result of this study, the efficient reproducible protocol of embryogenic callus formation and multiple shoot induction <i>in vitro</i> of <i>Paulownia tomentosa</i> was developed. This system provides a clear increase in the frequency of plant regeneration from 36.3 ± 3.4% to 38.6 ± 2.3% per embryogenic callus from leaves and apical shoot tips, respectively.https://www.mdpi.com/2223-7747/11/8/1020<i>Paulownia tomentosa</i>plant growth regulators<i>in vitro</i>somatic embryogenesisplant regeneration |
| spellingShingle | Aigul Amirova Symbat Dossymbetova Yeldana Rysbayeva Bakdaulet Usenbekov Arman Tolegen Alibek Ydyrys Multiple Plant Regeneration from Embryogenic Calli of <i>Paulownia tomentosa</i> (Thunb.) Steud Plants <i>Paulownia tomentosa</i> plant growth regulators <i>in vitro</i> somatic embryogenesis plant regeneration |
| title | Multiple Plant Regeneration from Embryogenic Calli of <i>Paulownia tomentosa</i> (Thunb.) Steud |
| title_full | Multiple Plant Regeneration from Embryogenic Calli of <i>Paulownia tomentosa</i> (Thunb.) Steud |
| title_fullStr | Multiple Plant Regeneration from Embryogenic Calli of <i>Paulownia tomentosa</i> (Thunb.) Steud |
| title_full_unstemmed | Multiple Plant Regeneration from Embryogenic Calli of <i>Paulownia tomentosa</i> (Thunb.) Steud |
| title_short | Multiple Plant Regeneration from Embryogenic Calli of <i>Paulownia tomentosa</i> (Thunb.) Steud |
| title_sort | multiple plant regeneration from embryogenic calli of i paulownia tomentosa i thunb steud |
| topic | <i>Paulownia tomentosa</i> plant growth regulators <i>in vitro</i> somatic embryogenesis plant regeneration |
| url | https://www.mdpi.com/2223-7747/11/8/1020 |
| work_keys_str_mv | AT aigulamirova multipleplantregenerationfromembryogeniccalliofipaulowniatomentosaithunbsteud AT symbatdossymbetova multipleplantregenerationfromembryogeniccalliofipaulowniatomentosaithunbsteud AT yeldanarysbayeva multipleplantregenerationfromembryogeniccalliofipaulowniatomentosaithunbsteud AT bakdauletusenbekov multipleplantregenerationfromembryogeniccalliofipaulowniatomentosaithunbsteud AT armantolegen multipleplantregenerationfromembryogeniccalliofipaulowniatomentosaithunbsteud AT alibekydyrys multipleplantregenerationfromembryogeniccalliofipaulowniatomentosaithunbsteud |