Prevalence of irregular red cell antibody in healthy blood donors attending a tertiary care hospital in North India
Background: Alloantibodies may be detected in blood donors who have either been transfused previously or female donors with previous obstetric events. These antibodies can occasionally cause severe transfusion reaction, if a large amount of plasma or whole blood is transfused, as in massive transfus...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2018-01-01
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| Series: | Asian Journal of Transfusion Science |
| Subjects: | |
| Online Access: | http://www.ajts.org/article.asp?issn=0973-6247;year=2018;volume=12;issue=1;spage=17;epage=20;aulast=Makroo |
| _version_ | 1828306996003078144 |
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| author | Raj Nath Makroo Saroj Rajput Soma Agarwal Mohit Chowdhry Bindu Prakash Prashant Karna |
| author_facet | Raj Nath Makroo Saroj Rajput Soma Agarwal Mohit Chowdhry Bindu Prakash Prashant Karna |
| author_sort | Raj Nath Makroo |
| collection | DOAJ |
| description | Background: Alloantibodies may be detected in blood donors who have either been transfused previously or female donors with previous obstetric events. These antibodies can occasionally cause severe transfusion reaction, if a large amount of plasma or whole blood is transfused, as in massive transfusions and pediatric patients.
Aims: The present study aims to assess the prevalence of red cell antibodies in healthy blood donors at a tertiary care hospital-based blood bank in India.
Materials And Methods: A total of 82,153 donor samples were screened for irregular red cell antibodies between January 2012 and December 2015 at the Department of Transfusion Medicine, Indraprastha Apollo Hospitals, New Delhi. Antibody screening was performed by solid phase method using Immucor Capture–R ready screen (pooled cells) on fully automated immunohematology analyzer Galileo Neo (Immucor Inc., Norcross, GA, USA). Positive tests were further confirmed using Capture-R ready screen (4 cell panel). Advanced investigations to identify the antibody/ies were performed on confirmed positive samples. Antibody identification was conducted using various cell panels (Immucor Capture-R Ready-ID, Panocell-10, Ficin Treated). An advanced technique such as adsorption and elution was performed as per requirement.
Results: Screening with pooled cells and 4 cell panel was positive in 227 donors (0.27%), 150 of these donors had autoantibodies, 1 had autoantibodies with underlying alloantibody anti-Jka (0.001%), and 76 had alloantibodies (0.09%) alone in their plasma. Anti-M was the most common antibody (43 donors) identified, followed by anti-D (21 donors). Anti-N was detected in 4; anti-Jka, anti-C, and anti-E in two donors each followed by anti-P1 and anti-Leb in 1 donor.
Conclusion: Antibodies against red cells can be present in healthy donors detection of which is important in providing safe blood to the patient. The prevalence of red blood cell antibody in healthy donors in this study was found to be 0.27%, while the prevalence of alloantibodies was 0.09%. The majority of alloantibodies were anti-M (56.57%) and anti-D (27.63%). |
| first_indexed | 2024-04-13T14:51:28Z |
| format | Article |
| id | doaj.art-5769ca7bb7eb41caa058367cee1b2696 |
| institution | Directory Open Access Journal |
| issn | 0973-6247 1998-3565 |
| language | English |
| last_indexed | 2024-04-13T14:51:28Z |
| publishDate | 2018-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Journal of Transfusion Science |
| spelling | doaj.art-5769ca7bb7eb41caa058367cee1b26962022-12-22T02:42:34ZengWolters Kluwer Medknow PublicationsAsian Journal of Transfusion Science0973-62471998-35652018-01-01121172010.4103/ajts.AJTS_4_17Prevalence of irregular red cell antibody in healthy blood donors attending a tertiary care hospital in North IndiaRaj Nath MakrooSaroj RajputSoma AgarwalMohit ChowdhryBindu PrakashPrashant KarnaBackground: Alloantibodies may be detected in blood donors who have either been transfused previously or female donors with previous obstetric events. These antibodies can occasionally cause severe transfusion reaction, if a large amount of plasma or whole blood is transfused, as in massive transfusions and pediatric patients. Aims: The present study aims to assess the prevalence of red cell antibodies in healthy blood donors at a tertiary care hospital-based blood bank in India. Materials And Methods: A total of 82,153 donor samples were screened for irregular red cell antibodies between January 2012 and December 2015 at the Department of Transfusion Medicine, Indraprastha Apollo Hospitals, New Delhi. Antibody screening was performed by solid phase method using Immucor Capture–R ready screen (pooled cells) on fully automated immunohematology analyzer Galileo Neo (Immucor Inc., Norcross, GA, USA). Positive tests were further confirmed using Capture-R ready screen (4 cell panel). Advanced investigations to identify the antibody/ies were performed on confirmed positive samples. Antibody identification was conducted using various cell panels (Immucor Capture-R Ready-ID, Panocell-10, Ficin Treated). An advanced technique such as adsorption and elution was performed as per requirement. Results: Screening with pooled cells and 4 cell panel was positive in 227 donors (0.27%), 150 of these donors had autoantibodies, 1 had autoantibodies with underlying alloantibody anti-Jka (0.001%), and 76 had alloantibodies (0.09%) alone in their plasma. Anti-M was the most common antibody (43 donors) identified, followed by anti-D (21 donors). Anti-N was detected in 4; anti-Jka, anti-C, and anti-E in two donors each followed by anti-P1 and anti-Leb in 1 donor. Conclusion: Antibodies against red cells can be present in healthy donors detection of which is important in providing safe blood to the patient. The prevalence of red blood cell antibody in healthy donors in this study was found to be 0.27%, while the prevalence of alloantibodies was 0.09%. The majority of alloantibodies were anti-M (56.57%) and anti-D (27.63%).http://www.ajts.org/article.asp?issn=0973-6247;year=2018;volume=12;issue=1;spage=17;epage=20;aulast=MakrooAlloantibody identificationalloantibody screeningdonor screening |
| spellingShingle | Raj Nath Makroo Saroj Rajput Soma Agarwal Mohit Chowdhry Bindu Prakash Prashant Karna Prevalence of irregular red cell antibody in healthy blood donors attending a tertiary care hospital in North India Asian Journal of Transfusion Science Alloantibody identification alloantibody screening donor screening |
| title | Prevalence of irregular red cell antibody in healthy blood donors attending a tertiary care hospital in North India |
| title_full | Prevalence of irregular red cell antibody in healthy blood donors attending a tertiary care hospital in North India |
| title_fullStr | Prevalence of irregular red cell antibody in healthy blood donors attending a tertiary care hospital in North India |
| title_full_unstemmed | Prevalence of irregular red cell antibody in healthy blood donors attending a tertiary care hospital in North India |
| title_short | Prevalence of irregular red cell antibody in healthy blood donors attending a tertiary care hospital in North India |
| title_sort | prevalence of irregular red cell antibody in healthy blood donors attending a tertiary care hospital in north india |
| topic | Alloantibody identification alloantibody screening donor screening |
| url | http://www.ajts.org/article.asp?issn=0973-6247;year=2018;volume=12;issue=1;spage=17;epage=20;aulast=Makroo |
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