Species Separation within, and Preliminary Phylogeny for, the Leafhopper Genus <i>Anoscopus</i> with Particular Reference to the Putative British Endemic <i>Anoscopus duffieldi</i> (Hemiptera: Cicadellidae)
The subfamily Aphrodinae (Hemiptera: Cicadellidae) contains ~33 species in Europe within four genera. Species in two genera in particular, <i>Aphrodes</i> and <i>Anoscopus</i>, have proved to be difficult to distinguish morphologically. Our aim was to determine the status of...
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MDPI AG
2020-11-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/11/11/799 |
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| author | Joanna Redihough Isa-Rita M. Russo Alan J. A. Stewart Igor Malenovský Jennifer E. Stockdale Rosemary J. Moorhouse-Gann Michael R. Wilson William O. C. Symondson |
| author_facet | Joanna Redihough Isa-Rita M. Russo Alan J. A. Stewart Igor Malenovský Jennifer E. Stockdale Rosemary J. Moorhouse-Gann Michael R. Wilson William O. C. Symondson |
| author_sort | Joanna Redihough |
| collection | DOAJ |
| description | The subfamily Aphrodinae (Hemiptera: Cicadellidae) contains ~33 species in Europe within four genera. Species in two genera in particular, <i>Aphrodes</i> and <i>Anoscopus</i>, have proved to be difficult to distinguish morphologically. Our aim was to determine the status of the putative species <i>Anoscopus duffieldi</i>, found only on the RSPB Nature Reserve at Dungeness, Kent, a possible rare UK endemic. DNA from samples of all seven UK <i>Anoscopus</i> species (plus <i>Anoscopus</i><i>alpinus</i> from the Czech Republic) were sequenced using parts of the mitochondrial cytochrome oxidase I and 16S rRNA genes. Bayesian inference phylogenies were created. Specimens of each species clustered into monophyletic groups, except for <i>Anoscopus</i><i>albifrons</i>, <i>A. duffieldi</i> and <i>Anoscopus</i><i>limicola</i>. Two <i>A. albifrons</i> specimens grouped with <i>A. duffieldi</i> repeatedly with strong support, and the remaining <i>A. albifrons</i> clustered within <i>A. limicola</i>. Genetic distances suggest that <i>A. albifrons</i> and <i>A. limicola</i> are a single interbreeding population (0% divergence), while <i>A. albifrons</i> and <i>A. duffieldi</i> diverged by only 0.28%. Shared haplotypes between <i>A. albifrons</i>, <i>A. limicola</i> and <i>A. duffieldi</i> strongly suggest interbreeding, although misidentification may also explain these topologies. However, all <i>A. duffieldi</i> clustered together in the trees. A conservative approach might be to treat <i>A. duffieldi</i>, until other evidence is forthcoming, as a possible endemic subspecies. |
| first_indexed | 2024-03-10T14:52:47Z |
| format | Article |
| id | doaj.art-576d2894916948d384e6f75bdd9425fe |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T14:52:47Z |
| publishDate | 2020-11-01 |
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| series | Insects |
| spelling | doaj.art-576d2894916948d384e6f75bdd9425fe2023-11-20T20:50:18ZengMDPI AGInsects2075-44502020-11-01111179910.3390/insects11110799Species Separation within, and Preliminary Phylogeny for, the Leafhopper Genus <i>Anoscopus</i> with Particular Reference to the Putative British Endemic <i>Anoscopus duffieldi</i> (Hemiptera: Cicadellidae)Joanna Redihough0Isa-Rita M. Russo1Alan J. A. Stewart2Igor Malenovský3Jennifer E. Stockdale4Rosemary J. Moorhouse-Gann5Michael R. Wilson6William O. C. Symondson7Cardiff School of Biosciences, Sir Martin Evans Building, Museum Avenue, Cardiff CF10 3AX, UKCardiff School of Biosciences, Sir Martin Evans Building, Museum Avenue, Cardiff CF10 3AX, UKSchool of Life Sciences, University of Sussex, Brighton BN1 9QG, East Sussex, UKDepartment of Botany and Zoology, Faculty of Science, Masaryk University, CZ-611 37 Brno, Czech RepublicCardiff School of Biosciences, Sir Martin Evans Building, Museum Avenue, Cardiff CF10 3AX, UKCardiff School of Biosciences, Sir Martin Evans Building, Museum Avenue, Cardiff CF10 3AX, UKDepartment of Natural Sciences, National Museum of Wales, Cardiff CF10 3NP, UKCardiff School of Biosciences, Sir Martin Evans Building, Museum Avenue, Cardiff CF10 3AX, UKThe subfamily Aphrodinae (Hemiptera: Cicadellidae) contains ~33 species in Europe within four genera. Species in two genera in particular, <i>Aphrodes</i> and <i>Anoscopus</i>, have proved to be difficult to distinguish morphologically. Our aim was to determine the status of the putative species <i>Anoscopus duffieldi</i>, found only on the RSPB Nature Reserve at Dungeness, Kent, a possible rare UK endemic. DNA from samples of all seven UK <i>Anoscopus</i> species (plus <i>Anoscopus</i><i>alpinus</i> from the Czech Republic) were sequenced using parts of the mitochondrial cytochrome oxidase I and 16S rRNA genes. Bayesian inference phylogenies were created. Specimens of each species clustered into monophyletic groups, except for <i>Anoscopus</i><i>albifrons</i>, <i>A. duffieldi</i> and <i>Anoscopus</i><i>limicola</i>. Two <i>A. albifrons</i> specimens grouped with <i>A. duffieldi</i> repeatedly with strong support, and the remaining <i>A. albifrons</i> clustered within <i>A. limicola</i>. Genetic distances suggest that <i>A. albifrons</i> and <i>A. limicola</i> are a single interbreeding population (0% divergence), while <i>A. albifrons</i> and <i>A. duffieldi</i> diverged by only 0.28%. Shared haplotypes between <i>A. albifrons</i>, <i>A. limicola</i> and <i>A. duffieldi</i> strongly suggest interbreeding, although misidentification may also explain these topologies. However, all <i>A. duffieldi</i> clustered together in the trees. A conservative approach might be to treat <i>A. duffieldi</i>, until other evidence is forthcoming, as a possible endemic subspecies.https://www.mdpi.com/2075-4450/11/11/799<i>Anoscopus duffieldi</i>AphrodinaeCicadellidaeendemic speciesgenetic distancemolecular separation |
| spellingShingle | Joanna Redihough Isa-Rita M. Russo Alan J. A. Stewart Igor Malenovský Jennifer E. Stockdale Rosemary J. Moorhouse-Gann Michael R. Wilson William O. C. Symondson Species Separation within, and Preliminary Phylogeny for, the Leafhopper Genus <i>Anoscopus</i> with Particular Reference to the Putative British Endemic <i>Anoscopus duffieldi</i> (Hemiptera: Cicadellidae) Insects <i>Anoscopus duffieldi</i> Aphrodinae Cicadellidae endemic species genetic distance molecular separation |
| title | Species Separation within, and Preliminary Phylogeny for, the Leafhopper Genus <i>Anoscopus</i> with Particular Reference to the Putative British Endemic <i>Anoscopus duffieldi</i> (Hemiptera: Cicadellidae) |
| title_full | Species Separation within, and Preliminary Phylogeny for, the Leafhopper Genus <i>Anoscopus</i> with Particular Reference to the Putative British Endemic <i>Anoscopus duffieldi</i> (Hemiptera: Cicadellidae) |
| title_fullStr | Species Separation within, and Preliminary Phylogeny for, the Leafhopper Genus <i>Anoscopus</i> with Particular Reference to the Putative British Endemic <i>Anoscopus duffieldi</i> (Hemiptera: Cicadellidae) |
| title_full_unstemmed | Species Separation within, and Preliminary Phylogeny for, the Leafhopper Genus <i>Anoscopus</i> with Particular Reference to the Putative British Endemic <i>Anoscopus duffieldi</i> (Hemiptera: Cicadellidae) |
| title_short | Species Separation within, and Preliminary Phylogeny for, the Leafhopper Genus <i>Anoscopus</i> with Particular Reference to the Putative British Endemic <i>Anoscopus duffieldi</i> (Hemiptera: Cicadellidae) |
| title_sort | species separation within and preliminary phylogeny for the leafhopper genus i anoscopus i with particular reference to the putative british endemic i anoscopus duffieldi i hemiptera cicadellidae |
| topic | <i>Anoscopus duffieldi</i> Aphrodinae Cicadellidae endemic species genetic distance molecular separation |
| url | https://www.mdpi.com/2075-4450/11/11/799 |
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