Suppression of damping-off disease causative agents using cellulase enzyme extracts of locally isolated cellulolytic fungi and their co-cultures
Damping-off disease is caused by <em>Pythium</em> sp., <em>Phytophthora</em> sp., <em>Rhizoctonia</em> sp., and <em>Fusarium</em> sp., and affects seedlings at the nursery stage. <em>Phytophthora</em> and <em>Pythium</em> cell w...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Faculty of Science, University of Peradeniya, Sri Lanka
2021-12-01
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| Series: | Ceylon Journal of Science |
| Subjects: | |
| Online Access: | https://cjs.sljol.info/articles/7947 |
| _version_ | 1797984478837080064 |
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| author | S. K. Jayasekara K. B. M. D. K. Karunarathna K. L. W. Kumara R. R. Ratnayake |
| author_facet | S. K. Jayasekara K. B. M. D. K. Karunarathna K. L. W. Kumara R. R. Ratnayake |
| author_sort | S. K. Jayasekara |
| collection | DOAJ |
| description | Damping-off disease is caused by <em>Pythium</em> sp., <em>Phytophthora</em> sp., <em>Rhizoctonia</em> sp., and <em>Fusarium</em> sp., and affects seedlings at the nursery stage. <em>Phytophthora</em> and <em>Pythium</em> cell walls contain cellulose. The current study aimed to determine potential use of cellulase extracts of selected fungal species <em>i.e.</em> <em>Talaromyces</em> sp., <em>Trichoderma</em> sp., <em>Aspergillus</em> sp., <em>A. niger</em>, and <em>Penicillium oxallicum</em> to suppress these pathogens and to investigate the potential use of fungal co-culture extracts with the highest cellulase activity for seed treatment to control damping-off. The impact of cellulase extracts on pathogen growth was determined by agar well diffusion method. For seed treatment, tomato seeds were soaked in cellulase extracts from the selected co-culture for 24 h and were sown in pathogens inoculated soil samples. Cellulase extracts of <em>Trichoderma</em> and <em>Aspergillus</em> suppressed <em>Phytophthora</em> growth with 63% and 57% of inhibition percentages respectively. When seeds were treated with <em>Talaromyces</em> sp.+<em>Aspergillus</em> sp.+<em>P. oxallicum</em> co-culture extracts, the highest seedling emergence percentage was observed in <em>Phytophthora</em> sp. <em>Fusarium</em> sp., and four-pathogen mixture-inoculated soils respectively. Lowest disease incidence was observed in <em>Phytophthora</em> sp. inoculated soil. Results show the potential of cellulolytic fungal species; <em>Trichoderma</em> sp., <em>Aspergillus</em> sp., and co-culture of <em>Talaromyces</em> sp.+<em>Aspergillus</em> sp.+<em>P. oxallicum</em> in suppression of <em>Phytophthora</em> sp. a common causative agent of damping-off. |
| first_indexed | 2024-04-11T07:02:20Z |
| format | Article |
| id | doaj.art-57738adc95ea4eabb2f28bb0309ca055 |
| institution | Directory Open Access Journal |
| issn | 2513-2814 2513-230X |
| language | English |
| last_indexed | 2024-04-11T07:02:20Z |
| publishDate | 2021-12-01 |
| publisher | Faculty of Science, University of Peradeniya, Sri Lanka |
| record_format | Article |
| series | Ceylon Journal of Science |
| spelling | doaj.art-57738adc95ea4eabb2f28bb0309ca0552022-12-22T04:38:35ZengFaculty of Science, University of Peradeniya, Sri LankaCeylon Journal of Science2513-28142513-230X2021-12-0150448749610.4038/cjs.v50i4.79475903Suppression of damping-off disease causative agents using cellulase enzyme extracts of locally isolated cellulolytic fungi and their co-culturesS. K. Jayasekara0K. B. M. D. K. Karunarathna1K. L. W. Kumara2R. R. Ratnayake3National Institute of Fundamental Studies, KandyUniversity of Ruhuna, Mapalana, KamburupitiyaUniversity of Ruhuna, Mapalana, KamburupitiyaNational Institute of Fundamental Studies, KandyDamping-off disease is caused by <em>Pythium</em> sp., <em>Phytophthora</em> sp., <em>Rhizoctonia</em> sp., and <em>Fusarium</em> sp., and affects seedlings at the nursery stage. <em>Phytophthora</em> and <em>Pythium</em> cell walls contain cellulose. The current study aimed to determine potential use of cellulase extracts of selected fungal species <em>i.e.</em> <em>Talaromyces</em> sp., <em>Trichoderma</em> sp., <em>Aspergillus</em> sp., <em>A. niger</em>, and <em>Penicillium oxallicum</em> to suppress these pathogens and to investigate the potential use of fungal co-culture extracts with the highest cellulase activity for seed treatment to control damping-off. The impact of cellulase extracts on pathogen growth was determined by agar well diffusion method. For seed treatment, tomato seeds were soaked in cellulase extracts from the selected co-culture for 24 h and were sown in pathogens inoculated soil samples. Cellulase extracts of <em>Trichoderma</em> and <em>Aspergillus</em> suppressed <em>Phytophthora</em> growth with 63% and 57% of inhibition percentages respectively. When seeds were treated with <em>Talaromyces</em> sp.+<em>Aspergillus</em> sp.+<em>P. oxallicum</em> co-culture extracts, the highest seedling emergence percentage was observed in <em>Phytophthora</em> sp. <em>Fusarium</em> sp., and four-pathogen mixture-inoculated soils respectively. Lowest disease incidence was observed in <em>Phytophthora</em> sp. inoculated soil. Results show the potential of cellulolytic fungal species; <em>Trichoderma</em> sp., <em>Aspergillus</em> sp., and co-culture of <em>Talaromyces</em> sp.+<em>Aspergillus</em> sp.+<em>P. oxallicum</em> in suppression of <em>Phytophthora</em> sp. a common causative agent of damping-off.https://cjs.sljol.info/articles/7947anti-pathogenic fungi, cellulolytic fungi, damping-off, phytophthora, seed treatment agents |
| spellingShingle | S. K. Jayasekara K. B. M. D. K. Karunarathna K. L. W. Kumara R. R. Ratnayake Suppression of damping-off disease causative agents using cellulase enzyme extracts of locally isolated cellulolytic fungi and their co-cultures Ceylon Journal of Science anti-pathogenic fungi, cellulolytic fungi, damping-off, phytophthora, seed treatment agents |
| title | Suppression of damping-off disease causative agents using cellulase enzyme extracts of locally isolated cellulolytic fungi and their co-cultures |
| title_full | Suppression of damping-off disease causative agents using cellulase enzyme extracts of locally isolated cellulolytic fungi and their co-cultures |
| title_fullStr | Suppression of damping-off disease causative agents using cellulase enzyme extracts of locally isolated cellulolytic fungi and their co-cultures |
| title_full_unstemmed | Suppression of damping-off disease causative agents using cellulase enzyme extracts of locally isolated cellulolytic fungi and their co-cultures |
| title_short | Suppression of damping-off disease causative agents using cellulase enzyme extracts of locally isolated cellulolytic fungi and their co-cultures |
| title_sort | suppression of damping off disease causative agents using cellulase enzyme extracts of locally isolated cellulolytic fungi and their co cultures |
| topic | anti-pathogenic fungi, cellulolytic fungi, damping-off, phytophthora, seed treatment agents |
| url | https://cjs.sljol.info/articles/7947 |
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