Fibroblast clearance of damaged tissue following laser ablation in engineered microtissues
Although the mechanisms underlying wound healing are largely preserved across wound types, the method of injury can affect the healing process. For example, burn wounds are more likely to undergo hypertrophic scarring than are lacerations, perhaps due to the increased underlying damage that needs to...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
AIP Publishing LLC
2023-03-01
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| Series: | APL Bioengineering |
| Online Access: | http://dx.doi.org/10.1063/5.0133478 |
| _version_ | 1797772653447086080 |
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| author | Megan Griebel Anish Vasan Christopher Chen Jeroen Eyckmans |
| author_facet | Megan Griebel Anish Vasan Christopher Chen Jeroen Eyckmans |
| author_sort | Megan Griebel |
| collection | DOAJ |
| description | Although the mechanisms underlying wound healing are largely preserved across wound types, the method of injury can affect the healing process. For example, burn wounds are more likely to undergo hypertrophic scarring than are lacerations, perhaps due to the increased underlying damage that needs to be cleared. This tissue clearance is thought to be mainly managed by immune cells, but it is unclear if fibroblasts contribute to this process. Herein, we utilize a 3D in vitro model of stromal wound healing to investigate the differences between two modes of injury: laceration and laser ablation. We demonstrate that laser ablation creates a ring of damaged tissue around the wound that is cleared by fibroblasts prior to wound closure. This process is dependent on ROCK and dynamin activity, suggesting a phagocytic or endocytic process. Transmission electron microscopy of fibroblasts that have entered the wound area reveals large intracellular vacuoles containing fibrillar extracellular matrix. These results demonstrate a new model to study matrix clearance by fibroblasts in a 3D soft tissue. Because aberrant wound healing is thought to be caused by an imbalance between matrix degradation and production, this model, which captures both aspects, will be a valuable addition to the study of wound healing. |
| first_indexed | 2024-03-12T21:54:03Z |
| format | Article |
| id | doaj.art-57747319c4d0471e8358dee1ed5383d6 |
| institution | Directory Open Access Journal |
| issn | 2473-2877 |
| language | English |
| last_indexed | 2024-03-12T21:54:03Z |
| publishDate | 2023-03-01 |
| publisher | AIP Publishing LLC |
| record_format | Article |
| series | APL Bioengineering |
| spelling | doaj.art-57747319c4d0471e8358dee1ed5383d62023-07-25T19:45:11ZengAIP Publishing LLCAPL Bioengineering2473-28772023-03-0171016112016112-910.1063/5.0133478Fibroblast clearance of damaged tissue following laser ablation in engineered microtissuesMegan Griebel0Anish Vasan1Christopher Chen2Jeroen Eyckmans3 Department of Biomedical Engineering and the Biological Design Center, Boston University, Boston, Massachusetts 02215, USA Department of Biomedical Engineering and the Biological Design Center, Boston University, Boston, Massachusetts 02215, USA Department of Biomedical Engineering and the Biological Design Center, Boston University, Boston, Massachusetts 02215, USA Department of Biomedical Engineering and the Biological Design Center, Boston University, Boston, Massachusetts 02215, USAAlthough the mechanisms underlying wound healing are largely preserved across wound types, the method of injury can affect the healing process. For example, burn wounds are more likely to undergo hypertrophic scarring than are lacerations, perhaps due to the increased underlying damage that needs to be cleared. This tissue clearance is thought to be mainly managed by immune cells, but it is unclear if fibroblasts contribute to this process. Herein, we utilize a 3D in vitro model of stromal wound healing to investigate the differences between two modes of injury: laceration and laser ablation. We demonstrate that laser ablation creates a ring of damaged tissue around the wound that is cleared by fibroblasts prior to wound closure. This process is dependent on ROCK and dynamin activity, suggesting a phagocytic or endocytic process. Transmission electron microscopy of fibroblasts that have entered the wound area reveals large intracellular vacuoles containing fibrillar extracellular matrix. These results demonstrate a new model to study matrix clearance by fibroblasts in a 3D soft tissue. Because aberrant wound healing is thought to be caused by an imbalance between matrix degradation and production, this model, which captures both aspects, will be a valuable addition to the study of wound healing.http://dx.doi.org/10.1063/5.0133478 |
| spellingShingle | Megan Griebel Anish Vasan Christopher Chen Jeroen Eyckmans Fibroblast clearance of damaged tissue following laser ablation in engineered microtissues APL Bioengineering |
| title | Fibroblast clearance of damaged tissue following laser ablation in engineered microtissues |
| title_full | Fibroblast clearance of damaged tissue following laser ablation in engineered microtissues |
| title_fullStr | Fibroblast clearance of damaged tissue following laser ablation in engineered microtissues |
| title_full_unstemmed | Fibroblast clearance of damaged tissue following laser ablation in engineered microtissues |
| title_short | Fibroblast clearance of damaged tissue following laser ablation in engineered microtissues |
| title_sort | fibroblast clearance of damaged tissue following laser ablation in engineered microtissues |
| url | http://dx.doi.org/10.1063/5.0133478 |
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