Xyloglucan and Concanavalin A based dressings in the topical treatment of mice wound healing process
For medical biomaterials, xyloglucan dispersions can form films or gels to be applied as a wound dressing. For this purpose, the structural characterization of xyloglucan dressing (XG) and xyloglucan dressing containing 0.5 mg/mL of concanavalin A (XGL) was performed. The lectin release capacity and...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-12-01
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| Series: | Carbohydrate Polymer Technologies and Applications |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666893921001043 |
| _version_ | 1818873875461570560 |
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| author | Isabel R.S. Arruda Marthyna P. Souza Paulo A.G. Soares Priscilla B.S. Albuquerque Túlio D. Silva Paloma L. Medeiros Marcia V. Silva Maria T.S. Correia António A. Vicente Maria G. Carneiro-da-Cunha |
| author_facet | Isabel R.S. Arruda Marthyna P. Souza Paulo A.G. Soares Priscilla B.S. Albuquerque Túlio D. Silva Paloma L. Medeiros Marcia V. Silva Maria T.S. Correia António A. Vicente Maria G. Carneiro-da-Cunha |
| author_sort | Isabel R.S. Arruda |
| collection | DOAJ |
| description | For medical biomaterials, xyloglucan dispersions can form films or gels to be applied as a wound dressing. For this purpose, the structural characterization of xyloglucan dressing (XG) and xyloglucan dressing containing 0.5 mg/mL of concanavalin A (XGL) was performed. The lectin release capacity and stability, cytotoxicity, and pro-wound healing effects were also investigated. XG and XGL films were prepared by mixing 0.5 % (w/v) xyloglucan with 0.3 % (v/v) glycerol. The ConA incorporated in the xyloglucan dressing maintained its biological activity for fourteen days in a controlled-release manner. The films were non-toxic, homogeneous, flexible, and accelerated the wound contraction compared with the control group, promoting less infiltration of inflammatory cells, angiogenesis, remodeling, and early epithelization. The films also alleviate the inflammation phase by reducing the production of pro-inflammatory cytokines (IFN-γ, TNF-α, IL-1β, IL-6, and IL-12), especially the XGL film, which promoted the up- and down-regulation of important proteins associated with the wound repair. All these findings suggest that XG and XGL films may represent a good therapeutic approach for wound healing applications. |
| first_indexed | 2024-12-19T13:01:39Z |
| format | Article |
| id | doaj.art-57766f91c147460dbf1203ea64798765 |
| institution | Directory Open Access Journal |
| issn | 2666-8939 |
| language | English |
| last_indexed | 2024-12-19T13:01:39Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Carbohydrate Polymer Technologies and Applications |
| spelling | doaj.art-57766f91c147460dbf1203ea647987652022-12-21T20:20:13ZengElsevierCarbohydrate Polymer Technologies and Applications2666-89392021-12-012100136Xyloglucan and Concanavalin A based dressings in the topical treatment of mice wound healing processIsabel R.S. Arruda0Marthyna P. Souza1Paulo A.G. Soares2Priscilla B.S. Albuquerque3Túlio D. Silva4Paloma L. Medeiros5Marcia V. Silva6Maria T.S. Correia7António A. Vicente8Maria G. Carneiro-da-Cunha9Departamento de Bioquímica, Universidade Federal de Pernambuco, Av. Prof. Moraes Rego s/n, CEP: 50.670-420 Recife, PE, BrasilDepartamento de Bioquímica, Universidade Federal de Pernambuco, Av. Prof. Moraes Rego s/n, CEP: 50.670-420 Recife, PE, Brasil; Corresponding authors at: Departamento de Bioquímica, Universidade Federal de Pernambuco-UFPE, Av. Prof. Moraes Rego, S/N, Cidade Universitária, CEP 50.670-420 Recife, PE, Brasil.Departamento de Bioquímica, Universidade Federal de Pernambuco, Av. Prof. Moraes Rego s/n, CEP: 50.670-420 Recife, PE, BrasilDepartamento de Medicina, Universidade de Pernambuco, campus Garanhuns, R. Cap. Pedro Rodrigues, São José, Garanhuns, Pernambuco CEP 55294-902, BrazilDepartamento de Bioquímica, Universidade Federal de Pernambuco, Av. Prof. Moraes Rego s/n, CEP: 50.670-420 Recife, PE, BrasilDepartamento de Histologia e Embriologia, Universidade Federal de Pernambuco, Av. Prof. Moraes Rego s/n, CEP: 50.670-420 Recife, PE, BrasilDepartamento de Bioquímica, Universidade Federal de Pernambuco, Av. Prof. Moraes Rego s/n, CEP: 50.670-420 Recife, PE, BrasilDepartamento de Bioquímica, Universidade Federal de Pernambuco, Av. Prof. Moraes Rego s/n, CEP: 50.670-420 Recife, PE, BrasilIBB–Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, PortugalDepartamento de Bioquímica, Universidade Federal de Pernambuco, Av. Prof. Moraes Rego s/n, CEP: 50.670-420 Recife, PE, Brasil; Laboratório de Imunopatologia Keizo Asami-LIKA, Universidade Federal de Pernambuco, Av. Prof. Moraes Rego s/n, CEP: 50.670-420 Recife, PE, Brasil; Corresponding authors at: Departamento de Bioquímica, Universidade Federal de Pernambuco-UFPE, Av. Prof. Moraes Rego, S/N, Cidade Universitária, CEP 50.670-420 Recife, PE, Brasil.For medical biomaterials, xyloglucan dispersions can form films or gels to be applied as a wound dressing. For this purpose, the structural characterization of xyloglucan dressing (XG) and xyloglucan dressing containing 0.5 mg/mL of concanavalin A (XGL) was performed. The lectin release capacity and stability, cytotoxicity, and pro-wound healing effects were also investigated. XG and XGL films were prepared by mixing 0.5 % (w/v) xyloglucan with 0.3 % (v/v) glycerol. The ConA incorporated in the xyloglucan dressing maintained its biological activity for fourteen days in a controlled-release manner. The films were non-toxic, homogeneous, flexible, and accelerated the wound contraction compared with the control group, promoting less infiltration of inflammatory cells, angiogenesis, remodeling, and early epithelization. The films also alleviate the inflammation phase by reducing the production of pro-inflammatory cytokines (IFN-γ, TNF-α, IL-1β, IL-6, and IL-12), especially the XGL film, which promoted the up- and down-regulation of important proteins associated with the wound repair. All these findings suggest that XG and XGL films may represent a good therapeutic approach for wound healing applications.http://www.sciencedirect.com/science/article/pii/S2666893921001043Polysaccharide filmsHymenaea courbarilFilm characterizationWound healingSerum proteome |
| spellingShingle | Isabel R.S. Arruda Marthyna P. Souza Paulo A.G. Soares Priscilla B.S. Albuquerque Túlio D. Silva Paloma L. Medeiros Marcia V. Silva Maria T.S. Correia António A. Vicente Maria G. Carneiro-da-Cunha Xyloglucan and Concanavalin A based dressings in the topical treatment of mice wound healing process Carbohydrate Polymer Technologies and Applications Polysaccharide films Hymenaea courbaril Film characterization Wound healing Serum proteome |
| title | Xyloglucan and Concanavalin A based dressings in the topical treatment of mice wound healing process |
| title_full | Xyloglucan and Concanavalin A based dressings in the topical treatment of mice wound healing process |
| title_fullStr | Xyloglucan and Concanavalin A based dressings in the topical treatment of mice wound healing process |
| title_full_unstemmed | Xyloglucan and Concanavalin A based dressings in the topical treatment of mice wound healing process |
| title_short | Xyloglucan and Concanavalin A based dressings in the topical treatment of mice wound healing process |
| title_sort | xyloglucan and concanavalin a based dressings in the topical treatment of mice wound healing process |
| topic | Polysaccharide films Hymenaea courbaril Film characterization Wound healing Serum proteome |
| url | http://www.sciencedirect.com/science/article/pii/S2666893921001043 |
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