Preparation and characterization of fibroin nanoparticles obtained from Bombyx mori L. Pilamo 1 cocoons
Silk fibroin (SF) is a biomacromolecule composed of proteins with properties, such as biocompatibility, biodegradability, and low immunogenicity. Thus, Silk fibroin nanoparticles (FNps) overcome the disadvantages of non-degradable synthetic nanoparticles. We studied the structural and thermal pro...
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| Format: | Article |
| Language: | English |
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Pontificia Universidad Javeriana
2022-10-01
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| Series: | Universitas Scientiarum |
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| Online Access: | https://revistas.javeriana.edu.co/index.php/scientarium/article/view/32773 |
| _version_ | 1797845467682308096 |
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| author | Luz Marina Baena Gloria Edith Guerrero Álvarez María Camila Giraldo González |
| author_facet | Luz Marina Baena Gloria Edith Guerrero Álvarez María Camila Giraldo González |
| author_sort | Luz Marina Baena |
| collection | DOAJ |
| description | Silk fibroin (SF) is a biomacromolecule composed of proteins with properties, such as biocompatibility,
biodegradability, and low immunogenicity. Thus, Silk fibroin nanoparticles (FNps) overcome the
disadvantages of non-degradable synthetic nanoparticles. We studied the structural and thermal
properties of SF and FNps from Bombyx mori L. cross-breed Pilamo I cocoons. Raw fibroin (RF) was
obtained using a sodium Na2CO3 solution as part of an experimental design to improve extraction, and
FNps were obtained by denaturing RF with a ternary solution of CaCl2:H2O:CH3CH2OH, followed
by precipitation using an anti-solvent method with propanol. Pilamo I cocoon, RF, and FNps were
characterized using Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy
(SEM), and elemental chemical analysis of energy dispersive X-rays (EDS). The Light Scattering
(DLS) and the thermal properties of RF and FNps were studied by thermogravimetric analysis (TGA)
and differential scanning calorimetry (DSC). The FTIR results showed that sericin-free raw fibroin
was obtained, and the SEM results showed that the nanometer-sized particles had a globular structure
and apparent porosity. The differences in the enthalpy of the crystallization peaks in the DSC and
TGA curves showed that the FNps had higher thermal stability than RF fibers. This result furthers the
development of alternative materials as vehicles of active compounds from natural extracts. |
| first_indexed | 2024-04-09T17:39:30Z |
| format | Article |
| id | doaj.art-577919fc6cef452399f51ee52e986646 |
| institution | Directory Open Access Journal |
| issn | 0122-7483 2027-1352 |
| language | English |
| last_indexed | 2024-04-09T17:39:30Z |
| publishDate | 2022-10-01 |
| publisher | Pontificia Universidad Javeriana |
| record_format | Article |
| series | Universitas Scientiarum |
| spelling | doaj.art-577919fc6cef452399f51ee52e9866462023-04-17T06:00:25ZengPontificia Universidad JaverianaUniversitas Scientiarum0122-74832027-13522022-10-0127327529010.11144/Javeriana.SC273.pacofPreparation and characterization of fibroin nanoparticles obtained from Bombyx mori L. Pilamo 1 cocoonsLuz Marina Baena0https://orcid.org/0000-0003-0792-9077Gloria Edith Guerrero Álvarez1https://orcid.org/0000-0002-0529-5835María Camila Giraldo González2https://orcid.org/0000-0002-0109-8439Universidad Tecnológica de Pereira, Faculty of Technology, School of Chemistry, Oleochemistry Research GroupUniversidad Tecnológica de Pereira, Faculty of Technology, School of Chemistry, Oleochemistry Research GroupUniversidad Tecnológica de Pereira, Faculty of Technology, School of Chemistry, Oleochemistry Research GroupSilk fibroin (SF) is a biomacromolecule composed of proteins with properties, such as biocompatibility, biodegradability, and low immunogenicity. Thus, Silk fibroin nanoparticles (FNps) overcome the disadvantages of non-degradable synthetic nanoparticles. We studied the structural and thermal properties of SF and FNps from Bombyx mori L. cross-breed Pilamo I cocoons. Raw fibroin (RF) was obtained using a sodium Na2CO3 solution as part of an experimental design to improve extraction, and FNps were obtained by denaturing RF with a ternary solution of CaCl2:H2O:CH3CH2OH, followed by precipitation using an anti-solvent method with propanol. Pilamo I cocoon, RF, and FNps were characterized using Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and elemental chemical analysis of energy dispersive X-rays (EDS). The Light Scattering (DLS) and the thermal properties of RF and FNps were studied by thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC). The FTIR results showed that sericin-free raw fibroin was obtained, and the SEM results showed that the nanometer-sized particles had a globular structure and apparent porosity. The differences in the enthalpy of the crystallization peaks in the DSC and TGA curves showed that the FNps had higher thermal stability than RF fibers. This result furthers the development of alternative materials as vehicles of active compounds from natural extracts.https://revistas.javeriana.edu.co/index.php/scientarium/article/view/32773fibroin;nanoparticles;ftir;sem;thermogravimetric analysis. |
| spellingShingle | Luz Marina Baena Gloria Edith Guerrero Álvarez María Camila Giraldo González Preparation and characterization of fibroin nanoparticles obtained from Bombyx mori L. Pilamo 1 cocoons Universitas Scientiarum fibroin; nanoparticles; ftir; sem; thermogravimetric analysis. |
| title | Preparation and characterization of fibroin nanoparticles obtained from Bombyx mori L. Pilamo 1 cocoons |
| title_full | Preparation and characterization of fibroin nanoparticles obtained from Bombyx mori L. Pilamo 1 cocoons |
| title_fullStr | Preparation and characterization of fibroin nanoparticles obtained from Bombyx mori L. Pilamo 1 cocoons |
| title_full_unstemmed | Preparation and characterization of fibroin nanoparticles obtained from Bombyx mori L. Pilamo 1 cocoons |
| title_short | Preparation and characterization of fibroin nanoparticles obtained from Bombyx mori L. Pilamo 1 cocoons |
| title_sort | preparation and characterization of fibroin nanoparticles obtained from bombyx mori l pilamo 1 cocoons |
| topic | fibroin; nanoparticles; ftir; sem; thermogravimetric analysis. |
| url | https://revistas.javeriana.edu.co/index.php/scientarium/article/view/32773 |
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