Explorative Field Study on the Use of Oral Fluids for the Surveillance of <i>Actinobacillus pleuropneumoniae</i> Infections in Fattening Farms by an Apx-Real-Time PCR
Oral fluids (OFs) represent a cost effective and reliable tool for surveillance purposes, mostly regarding viruses. In the present study, we evaluated the suitability of OFs for surveillance purposes concerning <i>Actinobacillus</i> (<i>A.</i>) <i>pleuropneumoniae</i...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/9/10/552 |
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| author | Michael Kleinmans Kerstin Fiebig Robert Tabeling Hanny Swam Annelies Duivelshof-Crienen Mathias Ritzmann Matthias Eddicks |
| author_facet | Michael Kleinmans Kerstin Fiebig Robert Tabeling Hanny Swam Annelies Duivelshof-Crienen Mathias Ritzmann Matthias Eddicks |
| author_sort | Michael Kleinmans |
| collection | DOAJ |
| description | Oral fluids (OFs) represent a cost effective and reliable tool for surveillance purposes, mostly regarding viruses. In the present study, we evaluated the suitability of OFs for surveillance purposes concerning <i>Actinobacillus</i> (<i>A.</i>) <i>pleuropneumoniae</i> infections in fattening pigs under field conditions. OFs were examined with an Apx-toxin real-time PCR that detects the genes encoding for Apx I-, Apx III-, and Apx IV-toxin. For this purpose, we conducted a pen-wise collection of OFs over one fattening period from fattening pigs of two farms (farm A and B) with a known history of <i>A. pleuropneumoniae</i> infection. Lung lesions were determined at slaughter to estimate the extend of pulmonary lesions and pleural affection. Apx III- and Apx IV-toxin DNA were present in the OFs of both farms whereas Apx I-toxin DNA was present on farm A only. We were able to detect Apx I-, Apx III-, and Apx IV-toxin DNA in different patterns directly after introduction of the new pigs in the farms and over the entire study period. In summary, or results indicate the suitability of OFS for the early detection and surveillance of <i>A. pleuropneumoniae</i> in fattening farms. |
| first_indexed | 2024-03-09T19:23:54Z |
| format | Article |
| id | doaj.art-5779257622ee42c29bdb41d1e05a89ea |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-09T19:23:54Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-5779257622ee42c29bdb41d1e05a89ea2023-11-24T03:06:33ZengMDPI AGVeterinary Sciences2306-73812022-10-0191055210.3390/vetsci9100552Explorative Field Study on the Use of Oral Fluids for the Surveillance of <i>Actinobacillus pleuropneumoniae</i> Infections in Fattening Farms by an Apx-Real-Time PCRMichael Kleinmans0Kerstin Fiebig1Robert Tabeling2Hanny Swam3Annelies Duivelshof-Crienen4Mathias Ritzmann5Matthias Eddicks6Veterinary Practice “Am Kapellhof”, 47608 Geldern, GermanyIntervet Deutschland GmbH, MSD Animal Health, 85716 Unterschleissheim, GermanyIntervet Deutschland GmbH, MSD Animal Health, 85716 Unterschleissheim, GermanyIntervet International B.V., 5831 AK Boxmeer, The NetherlandsIntervet International B.V., 5831 AK Boxmeer, The NetherlandsClinic for Swine at the Centre for Clinical Veterinary Medicine, Ludwig-Maximilians-Universität München, 85764 Oberschleissheim, GermanyClinic for Swine at the Centre for Clinical Veterinary Medicine, Ludwig-Maximilians-Universität München, 85764 Oberschleissheim, GermanyOral fluids (OFs) represent a cost effective and reliable tool for surveillance purposes, mostly regarding viruses. In the present study, we evaluated the suitability of OFs for surveillance purposes concerning <i>Actinobacillus</i> (<i>A.</i>) <i>pleuropneumoniae</i> infections in fattening pigs under field conditions. OFs were examined with an Apx-toxin real-time PCR that detects the genes encoding for Apx I-, Apx III-, and Apx IV-toxin. For this purpose, we conducted a pen-wise collection of OFs over one fattening period from fattening pigs of two farms (farm A and B) with a known history of <i>A. pleuropneumoniae</i> infection. Lung lesions were determined at slaughter to estimate the extend of pulmonary lesions and pleural affection. Apx III- and Apx IV-toxin DNA were present in the OFs of both farms whereas Apx I-toxin DNA was present on farm A only. We were able to detect Apx I-, Apx III-, and Apx IV-toxin DNA in different patterns directly after introduction of the new pigs in the farms and over the entire study period. In summary, or results indicate the suitability of OFS for the early detection and surveillance of <i>A. pleuropneumoniae</i> in fattening farms.https://www.mdpi.com/2306-7381/9/10/552surveillancetransmissionfattening |
| spellingShingle | Michael Kleinmans Kerstin Fiebig Robert Tabeling Hanny Swam Annelies Duivelshof-Crienen Mathias Ritzmann Matthias Eddicks Explorative Field Study on the Use of Oral Fluids for the Surveillance of <i>Actinobacillus pleuropneumoniae</i> Infections in Fattening Farms by an Apx-Real-Time PCR Veterinary Sciences surveillance transmission fattening |
| title | Explorative Field Study on the Use of Oral Fluids for the Surveillance of <i>Actinobacillus pleuropneumoniae</i> Infections in Fattening Farms by an Apx-Real-Time PCR |
| title_full | Explorative Field Study on the Use of Oral Fluids for the Surveillance of <i>Actinobacillus pleuropneumoniae</i> Infections in Fattening Farms by an Apx-Real-Time PCR |
| title_fullStr | Explorative Field Study on the Use of Oral Fluids for the Surveillance of <i>Actinobacillus pleuropneumoniae</i> Infections in Fattening Farms by an Apx-Real-Time PCR |
| title_full_unstemmed | Explorative Field Study on the Use of Oral Fluids for the Surveillance of <i>Actinobacillus pleuropneumoniae</i> Infections in Fattening Farms by an Apx-Real-Time PCR |
| title_short | Explorative Field Study on the Use of Oral Fluids for the Surveillance of <i>Actinobacillus pleuropneumoniae</i> Infections in Fattening Farms by an Apx-Real-Time PCR |
| title_sort | explorative field study on the use of oral fluids for the surveillance of i actinobacillus pleuropneumoniae i infections in fattening farms by an apx real time pcr |
| topic | surveillance transmission fattening |
| url | https://www.mdpi.com/2306-7381/9/10/552 |
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