Activation of gene expression by histone deubiquitinase OTLD1
One of the main mechanisms of epigenetic control is post translational modification of histones, and one of the relatively less characterized, yet functionally important histone modifications is monoubiquitylation, which is reversed by histone deubiquitinases. In Arabidopsis, only two of such enzyme...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2017-07-01
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| Series: | Epigenetics |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/15592294.2017.1348446 |
| _version_ | 1797678954081943552 |
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| author | Ido Keren Vitaly Citovsky |
| author_facet | Ido Keren Vitaly Citovsky |
| author_sort | Ido Keren |
| collection | DOAJ |
| description | One of the main mechanisms of epigenetic control is post translational modification of histones, and one of the relatively less characterized, yet functionally important histone modifications is monoubiquitylation, which is reversed by histone deubiquitinases. In Arabidopsis, only two of such enzymes are known to date. One of them, OTLD1, deubiquitylates histone 2B and functions as a transcriptional repressor. But, could the same deubiquitinase act both as a repressor and an activator? Here, we addressed this question. Using gain-of-function and loss-of-function Arabidopsis alleles, we showed that OTLD1 can promote expression of a target gene. This transcriptional activation activity of OTLD1 involves occupation of the target chromatin by this enzyme, deubiquitination of monoubiquitylated H2B within the occupied regions, and formation of the euchromatic histone acetylation and methylation marks. Thus, OTLD1 can play a dual role in transcriptional repression and activation of its target genes. In these reactions, H2B ubiquitylation acts as both a repressive and an active mark whereas OTLD1 association with and deubiquitylation of the target chromatin may represent the key juncture between two opposing effects of this enzyme on gene expression. |
| first_indexed | 2024-03-11T23:07:23Z |
| format | Article |
| id | doaj.art-5779729fa7804093910c91159a2e6dbd |
| institution | Directory Open Access Journal |
| issn | 1559-2294 1559-2308 |
| language | English |
| last_indexed | 2024-03-11T23:07:23Z |
| publishDate | 2017-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Epigenetics |
| spelling | doaj.art-5779729fa7804093910c91159a2e6dbd2023-09-21T12:43:13ZengTaylor & Francis GroupEpigenetics1559-22941559-23082017-07-0112758459010.1080/15592294.2017.13484461348446Activation of gene expression by histone deubiquitinase OTLD1Ido Keren0Vitaly Citovsky1State University of New YorkState University of New YorkOne of the main mechanisms of epigenetic control is post translational modification of histones, and one of the relatively less characterized, yet functionally important histone modifications is monoubiquitylation, which is reversed by histone deubiquitinases. In Arabidopsis, only two of such enzymes are known to date. One of them, OTLD1, deubiquitylates histone 2B and functions as a transcriptional repressor. But, could the same deubiquitinase act both as a repressor and an activator? Here, we addressed this question. Using gain-of-function and loss-of-function Arabidopsis alleles, we showed that OTLD1 can promote expression of a target gene. This transcriptional activation activity of OTLD1 involves occupation of the target chromatin by this enzyme, deubiquitination of monoubiquitylated H2B within the occupied regions, and formation of the euchromatic histone acetylation and methylation marks. Thus, OTLD1 can play a dual role in transcriptional repression and activation of its target genes. In these reactions, H2B ubiquitylation acts as both a repressive and an active mark whereas OTLD1 association with and deubiquitylation of the target chromatin may represent the key juncture between two opposing effects of this enzyme on gene expression.http://dx.doi.org/10.1080/15592294.2017.1348446chromatinchromatin remodelingepigeneticshistone modifications |
| spellingShingle | Ido Keren Vitaly Citovsky Activation of gene expression by histone deubiquitinase OTLD1 Epigenetics chromatin chromatin remodeling epigenetics histone modifications |
| title | Activation of gene expression by histone deubiquitinase OTLD1 |
| title_full | Activation of gene expression by histone deubiquitinase OTLD1 |
| title_fullStr | Activation of gene expression by histone deubiquitinase OTLD1 |
| title_full_unstemmed | Activation of gene expression by histone deubiquitinase OTLD1 |
| title_short | Activation of gene expression by histone deubiquitinase OTLD1 |
| title_sort | activation of gene expression by histone deubiquitinase otld1 |
| topic | chromatin chromatin remodeling epigenetics histone modifications |
| url | http://dx.doi.org/10.1080/15592294.2017.1348446 |
| work_keys_str_mv | AT idokeren activationofgeneexpressionbyhistonedeubiquitinaseotld1 AT vitalycitovsky activationofgeneexpressionbyhistonedeubiquitinaseotld1 |