A versatile integrated tube for rapid and visual SARS-CoV-2 detection
The coronavirus disease 2019 (COVID-19) caused by novel severe acute respiratory coronavirus 2 (SARS-CoV-2) has been rapidly spreading worldwide. Rapid and widespread testing is essential to promote early intervention and curb the ongoing COVID-19 pandemic. Current gold standard reverse transcriptio...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1070831/full |
| _version_ | 1797955203327066112 |
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| author | Jingsong Xu Xi Wang Shuang Yang Lei He Yuting Wang Yuting Wang Jiajun Li Qian Liu Min Li Hua Wang |
| author_facet | Jingsong Xu Xi Wang Shuang Yang Lei He Yuting Wang Yuting Wang Jiajun Li Qian Liu Min Li Hua Wang |
| author_sort | Jingsong Xu |
| collection | DOAJ |
| description | The coronavirus disease 2019 (COVID-19) caused by novel severe acute respiratory coronavirus 2 (SARS-CoV-2) has been rapidly spreading worldwide. Rapid and widespread testing is essential to promote early intervention and curb the ongoing COVID-19 pandemic. Current gold standard reverse transcription-polymerase chain reaction (RT-PCR) for detecting SARS-CoV-2 is restricted to professional laboratories and well-trained personnel, thus, limiting its widespread use in resource-limited conditions. To overcome these challenges, we developed a rapid and convenient assay using a versatile integrated tube for the rapid and visual detection of SARS-CoV-2. The reaction conditions of the method were optimized using SARS-CoV-2 RNA standards and the sensitivity and specificity were further determined. Finally, it was verified on clinical specimens. The assay was completed within 40 min, and the result was visible by the naked eye. The limits of detection (LODs) for the target ORF1ab and N genes were 50 copies/μl. No cross-reactivity was observed with the RNA standard samples of four respiratory viruses or clinical samples of common respiratory viral infections. Ninety SARS-CoV-2 positive and 30 SARS-CoV-2 negative patient specimens were analyzed. We compared these results to both prior and concurrent RT-PCR evaluations. As a result, the overall sensitivity and specificity for detection SARS-CoV-2 were 94.5 and 100.0%, respectively.ConclusionThe integrated tube assay has the potential to provide a simple, specific, sensitive, one-pot, and single-step assay for SARS-CoV-2. |
| first_indexed | 2024-04-10T23:29:46Z |
| format | Article |
| id | doaj.art-5779c9af46874d03bec78c2900929027 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-10T23:29:46Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-5779c9af46874d03bec78c29009290272023-01-12T07:02:39ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-01-011310.3389/fmicb.2022.10708311070831A versatile integrated tube for rapid and visual SARS-CoV-2 detectionJingsong Xu0Xi Wang1Shuang Yang2Lei He3Yuting Wang4Yuting Wang5Jiajun Li6Qian Liu7Min Li8Hua Wang9Department of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaShanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaDepartment of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaThe coronavirus disease 2019 (COVID-19) caused by novel severe acute respiratory coronavirus 2 (SARS-CoV-2) has been rapidly spreading worldwide. Rapid and widespread testing is essential to promote early intervention and curb the ongoing COVID-19 pandemic. Current gold standard reverse transcription-polymerase chain reaction (RT-PCR) for detecting SARS-CoV-2 is restricted to professional laboratories and well-trained personnel, thus, limiting its widespread use in resource-limited conditions. To overcome these challenges, we developed a rapid and convenient assay using a versatile integrated tube for the rapid and visual detection of SARS-CoV-2. The reaction conditions of the method were optimized using SARS-CoV-2 RNA standards and the sensitivity and specificity were further determined. Finally, it was verified on clinical specimens. The assay was completed within 40 min, and the result was visible by the naked eye. The limits of detection (LODs) for the target ORF1ab and N genes were 50 copies/μl. No cross-reactivity was observed with the RNA standard samples of four respiratory viruses or clinical samples of common respiratory viral infections. Ninety SARS-CoV-2 positive and 30 SARS-CoV-2 negative patient specimens were analyzed. We compared these results to both prior and concurrent RT-PCR evaluations. As a result, the overall sensitivity and specificity for detection SARS-CoV-2 were 94.5 and 100.0%, respectively.ConclusionThe integrated tube assay has the potential to provide a simple, specific, sensitive, one-pot, and single-step assay for SARS-CoV-2.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1070831/fullintegrated tubeSARS-CoV-2CRISPR-Cas12arecombinase polymerase amplificationvisual detection |
| spellingShingle | Jingsong Xu Xi Wang Shuang Yang Lei He Yuting Wang Yuting Wang Jiajun Li Qian Liu Min Li Hua Wang A versatile integrated tube for rapid and visual SARS-CoV-2 detection Frontiers in Microbiology integrated tube SARS-CoV-2 CRISPR-Cas12a recombinase polymerase amplification visual detection |
| title | A versatile integrated tube for rapid and visual SARS-CoV-2 detection |
| title_full | A versatile integrated tube for rapid and visual SARS-CoV-2 detection |
| title_fullStr | A versatile integrated tube for rapid and visual SARS-CoV-2 detection |
| title_full_unstemmed | A versatile integrated tube for rapid and visual SARS-CoV-2 detection |
| title_short | A versatile integrated tube for rapid and visual SARS-CoV-2 detection |
| title_sort | versatile integrated tube for rapid and visual sars cov 2 detection |
| topic | integrated tube SARS-CoV-2 CRISPR-Cas12a recombinase polymerase amplification visual detection |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1070831/full |
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