Effect of natural selection on common bean (Phaseolus vulgaris) microsatellite alleles
The effect of natural selection on microsatellite simple sequence repeat (SSR) alleles was investigated in two distinct common bean (Phaseolus vulgaris) generations (F8 and F24) derived from the cross between the P. vulgaris cultivars Carioca MG x ESAL 686. The F2 segregant population was propagated...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2006-01-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200024 |
| _version_ | 1818765924873797632 |
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| author | Taislene Butarello Rodrigues João Bosco dos Santos |
| author_facet | Taislene Butarello Rodrigues João Bosco dos Santos |
| author_sort | Taislene Butarello Rodrigues |
| collection | DOAJ |
| description | The effect of natural selection on microsatellite simple sequence repeat (SSR) alleles was investigated in two distinct common bean (Phaseolus vulgaris) generations (F8 and F24) derived from the cross between the P. vulgaris cultivars Carioca MG x ESAL 686. The F2 segregant population was propagated by the bulk method and 107 plants were sampled in two generations (F8 and F24). Each plant generated one family which was replicated by the bulk method to F8:11 and F24:27 families from which DNA was extracted. Thirty pairs of microsatellite primers were polymorphic for the parents and the bulk of the F24:27 families. Out of 30 loci selected by natural selection, 29 microsatellite alleles came from the Carioca MG parent and one allele came from the ESAL 686 parent. Natural selection affected all the generations and its intensity was specific for each locus and generation. Therefore all the alleles selected at each locus must be important for adaptation in a breeding program. |
| first_indexed | 2024-12-18T08:25:50Z |
| format | Article |
| id | doaj.art-577a0d0e884f4e459c805afb3ec9bf93 |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-18T08:25:50Z |
| publishDate | 2006-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-577a0d0e884f4e459c805afb3ec9bf932022-12-21T21:14:37ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852006-01-0129234535210.1590/S1415-47572006000200024Effect of natural selection on common bean (Phaseolus vulgaris) microsatellite allelesTaislene Butarello RodriguesJoão Bosco dos SantosThe effect of natural selection on microsatellite simple sequence repeat (SSR) alleles was investigated in two distinct common bean (Phaseolus vulgaris) generations (F8 and F24) derived from the cross between the P. vulgaris cultivars Carioca MG x ESAL 686. The F2 segregant population was propagated by the bulk method and 107 plants were sampled in two generations (F8 and F24). Each plant generated one family which was replicated by the bulk method to F8:11 and F24:27 families from which DNA was extracted. Thirty pairs of microsatellite primers were polymorphic for the parents and the bulk of the F24:27 families. Out of 30 loci selected by natural selection, 29 microsatellite alleles came from the Carioca MG parent and one allele came from the ESAL 686 parent. Natural selection affected all the generations and its intensity was specific for each locus and generation. Therefore all the alleles selected at each locus must be important for adaptation in a breeding program.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200024natural selectionmicrosatellite markersPhaseolus vulgarisadaptation |
| spellingShingle | Taislene Butarello Rodrigues João Bosco dos Santos Effect of natural selection on common bean (Phaseolus vulgaris) microsatellite alleles Genetics and Molecular Biology natural selection microsatellite markers Phaseolus vulgaris adaptation |
| title | Effect of natural selection on common bean (Phaseolus vulgaris) microsatellite alleles |
| title_full | Effect of natural selection on common bean (Phaseolus vulgaris) microsatellite alleles |
| title_fullStr | Effect of natural selection on common bean (Phaseolus vulgaris) microsatellite alleles |
| title_full_unstemmed | Effect of natural selection on common bean (Phaseolus vulgaris) microsatellite alleles |
| title_short | Effect of natural selection on common bean (Phaseolus vulgaris) microsatellite alleles |
| title_sort | effect of natural selection on common bean phaseolus vulgaris microsatellite alleles |
| topic | natural selection microsatellite markers Phaseolus vulgaris adaptation |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200024 |
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