Kinetic Changes in B7 Costimulatory Molecules and IRF4 Expression in Human Dendritic Cells during LPS Exposure
A key aspect of the inflammatory phenomenon is the involvement of costimulatory molecules expressed by antigen-presenting cells (APCs) and their ability to secrete cytokines to set instructions for an adaptive immune response and to generate tolerance or inflammation. In a novel integrative approach...
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| Format: | Article |
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MDPI AG
2022-07-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/12/7/955 |
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| author | Henry Velazquez-Soto Fernanda Real-San Miguel Sonia Mayra Pérez-Tapia María C. Jiménez-Martínez |
| author_facet | Henry Velazquez-Soto Fernanda Real-San Miguel Sonia Mayra Pérez-Tapia María C. Jiménez-Martínez |
| author_sort | Henry Velazquez-Soto |
| collection | DOAJ |
| description | A key aspect of the inflammatory phenomenon is the involvement of costimulatory molecules expressed by antigen-presenting cells (APCs) and their ability to secrete cytokines to set instructions for an adaptive immune response and to generate tolerance or inflammation. In a novel integrative approach, we aimed to evaluate the kinetic expression of the membrane and soluble B7 costimulatory molecules CD86, ICOS-L, PDL1, PDL2, the transcription factor Interferon Regulatory Factor 4 (IRF4), and the cytokines produced by monocyte-derived dendritic cells (Mo-DCs) after challenging them with different concentrations of stimulation with <i>E. coli</i> lipopolysaccharide (LPS) for different lengths of time. Our results showed that the stimuli concentration and time of exposure to an antigen are key factors in modulating the dynamic expression pattern of membrane and soluble B7 molecules and cytokines. |
| first_indexed | 2024-03-09T12:10:40Z |
| format | Article |
| id | doaj.art-577e17081f0b49429477c4031bc5e9ca |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-09T12:10:40Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-577e17081f0b49429477c4031bc5e9ca2023-11-30T22:52:24ZengMDPI AGBiomolecules2218-273X2022-07-0112795510.3390/biom12070955Kinetic Changes in B7 Costimulatory Molecules and IRF4 Expression in Human Dendritic Cells during LPS ExposureHenry Velazquez-Soto0Fernanda Real-San Miguel1Sonia Mayra Pérez-Tapia2María C. Jiménez-Martínez3Department of Immunology and Research Unit, Institute of Ophthalmology “Conde de Valenciana Foundation”, Mexico City 06800, MexicoDepartment of Immunology and Research Unit, Institute of Ophthalmology “Conde de Valenciana Foundation”, Mexico City 06800, MexicoUnidad de Desarrollo e Investigación en Bioprocesos (UDIBI), Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City 11340, MexicoDepartment of Immunology and Research Unit, Institute of Ophthalmology “Conde de Valenciana Foundation”, Mexico City 06800, MexicoA key aspect of the inflammatory phenomenon is the involvement of costimulatory molecules expressed by antigen-presenting cells (APCs) and their ability to secrete cytokines to set instructions for an adaptive immune response and to generate tolerance or inflammation. In a novel integrative approach, we aimed to evaluate the kinetic expression of the membrane and soluble B7 costimulatory molecules CD86, ICOS-L, PDL1, PDL2, the transcription factor Interferon Regulatory Factor 4 (IRF4), and the cytokines produced by monocyte-derived dendritic cells (Mo-DCs) after challenging them with different concentrations of stimulation with <i>E. coli</i> lipopolysaccharide (LPS) for different lengths of time. Our results showed that the stimuli concentration and time of exposure to an antigen are key factors in modulating the dynamic expression pattern of membrane and soluble B7 molecules and cytokines.https://www.mdpi.com/2218-273X/12/7/955LPScostimulatory moleculesdendritic cellscytokinesPDL1PDL2 |
| spellingShingle | Henry Velazquez-Soto Fernanda Real-San Miguel Sonia Mayra Pérez-Tapia María C. Jiménez-Martínez Kinetic Changes in B7 Costimulatory Molecules and IRF4 Expression in Human Dendritic Cells during LPS Exposure Biomolecules LPS costimulatory molecules dendritic cells cytokines PDL1 PDL2 |
| title | Kinetic Changes in B7 Costimulatory Molecules and IRF4 Expression in Human Dendritic Cells during LPS Exposure |
| title_full | Kinetic Changes in B7 Costimulatory Molecules and IRF4 Expression in Human Dendritic Cells during LPS Exposure |
| title_fullStr | Kinetic Changes in B7 Costimulatory Molecules and IRF4 Expression in Human Dendritic Cells during LPS Exposure |
| title_full_unstemmed | Kinetic Changes in B7 Costimulatory Molecules and IRF4 Expression in Human Dendritic Cells during LPS Exposure |
| title_short | Kinetic Changes in B7 Costimulatory Molecules and IRF4 Expression in Human Dendritic Cells during LPS Exposure |
| title_sort | kinetic changes in b7 costimulatory molecules and irf4 expression in human dendritic cells during lps exposure |
| topic | LPS costimulatory molecules dendritic cells cytokines PDL1 PDL2 |
| url | https://www.mdpi.com/2218-273X/12/7/955 |
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