Evaluation of Cytotoxicity of Endoseal MTA Sealer as a Root Canal Perforation Restorative Material on Gingival Fibroblasts Compared to Iranian ProRoot MTA and CEM by MTT test
Introduction: Root canal perforation is a reason for root canal treatment failures. Cytocompatibility is an essential feature of a perforation restorative material. This in vitro study aimed to evaluate the cytotoxicity of Endoseal MTA sealer as a root canal perforation restorative material on human...
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Mashhad University of Medical Sciences
2022-12-01
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| Series: | Journal of Mashhad Dental School |
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| Online Access: | https://jmds.mums.ac.ir/article_21406_4f2fadcc6578b60d13b00b6e2547d1e3.pdf |
| _version_ | 1797978013590093824 |
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| author | Salma Omidi Kosar Dadgar Abbas Mesgarani Mahmoud Moosazade Fatemeh Zaghi Hosseinzadeh |
| author_facet | Salma Omidi Kosar Dadgar Abbas Mesgarani Mahmoud Moosazade Fatemeh Zaghi Hosseinzadeh |
| author_sort | Salma Omidi |
| collection | DOAJ |
| description | Introduction: Root canal perforation is a reason for root canal treatment failures. Cytocompatibility is an essential feature of a perforation restorative material. This in vitro study aimed to evaluate the cytotoxicity of Endoseal MTA sealer as a root canal perforation restorative material on human gingival fibroblast cells in comparison to Iranian ProRoot MTA and CEM using tetrazolium-based colorimetric assay (MTT test). Materials and Methods: MTA, CEM, and Endoseal MTA were prepared and exposed (for 48 h) to cell culture media 72 h after setting. Human gingival fibroblasts were then cultivated and incubated for 24 h with different dilutions (1/2, 1/4, 1/8) of each sealer extract. Cell viability was evaluated using the MTT test. The data were compared using the analysis of variance (ANOVA) followed by Tukey’s post hoc test for multiple comparisons. P-values less than 0.05 were considered statistically significant.Result: There was a significant association between CEM cell viability and Endoseal MTA as well as between MTA and CEM in 1/2 dilution. No significant difference was found in 1/4 and 1/8 dilusions between the percentage of cell viability of different sealers.Conclusion: Based on the results of this study, although Endoseal MTA sealer is more toxic than CEM in ½ dilution, it has higher biocompatibility compared to MTA and can be considered as a perforation restorative material in terms of other favorable qualities. |
| first_indexed | 2024-04-11T05:16:12Z |
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| id | doaj.art-577e5090f37649d7a66c840c4bd91054 |
| institution | Directory Open Access Journal |
| issn | 1560-9286 2008-2347 |
| language | fas |
| last_indexed | 2024-04-11T05:16:12Z |
| publishDate | 2022-12-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Journal of Mashhad Dental School |
| spelling | doaj.art-577e5090f37649d7a66c840c4bd910542022-12-24T10:16:47ZfasMashhad University of Medical SciencesJournal of Mashhad Dental School1560-92862008-23472022-12-0146430531510.22038/jmds.2022.58366.206321406Evaluation of Cytotoxicity of Endoseal MTA Sealer as a Root Canal Perforation Restorative Material on Gingival Fibroblasts Compared to Iranian ProRoot MTA and CEM by MTT testSalma Omidi0Kosar Dadgar1Abbas Mesgarani2Mahmoud Moosazade3Fatemeh Zaghi Hosseinzadeh4Assistant Professor, Department of Endodontics, School of Dentistry, Mazandaran University of Medical Sciences, Sari, IranAssistant Professor, Department of Prosthodontics, School of Dentistry, Mazandaran University of Medical Sciences, Sari, Iran,Associate Professor, Department of Endodontics, School of Dentistry, Mazandaran University of Medical Sciences, Sari, IranAssistant Professor, Deapartment of Epidemiology, Health Sciences Research Center, Addiction Institute, Mazandaran University of Medical Sciences, Sari, Iran Gastrointestinal Cancer Research Centre, Non-communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, IranDentist, Sari, IranIntroduction: Root canal perforation is a reason for root canal treatment failures. Cytocompatibility is an essential feature of a perforation restorative material. This in vitro study aimed to evaluate the cytotoxicity of Endoseal MTA sealer as a root canal perforation restorative material on human gingival fibroblast cells in comparison to Iranian ProRoot MTA and CEM using tetrazolium-based colorimetric assay (MTT test). Materials and Methods: MTA, CEM, and Endoseal MTA were prepared and exposed (for 48 h) to cell culture media 72 h after setting. Human gingival fibroblasts were then cultivated and incubated for 24 h with different dilutions (1/2, 1/4, 1/8) of each sealer extract. Cell viability was evaluated using the MTT test. The data were compared using the analysis of variance (ANOVA) followed by Tukey’s post hoc test for multiple comparisons. P-values less than 0.05 were considered statistically significant.Result: There was a significant association between CEM cell viability and Endoseal MTA as well as between MTA and CEM in 1/2 dilution. No significant difference was found in 1/4 and 1/8 dilusions between the percentage of cell viability of different sealers.Conclusion: Based on the results of this study, although Endoseal MTA sealer is more toxic than CEM in ½ dilution, it has higher biocompatibility compared to MTA and can be considered as a perforation restorative material in terms of other favorable qualities.https://jmds.mums.ac.ir/article_21406_4f2fadcc6578b60d13b00b6e2547d1e3.pdfcemcytotoxicityendoseal mtamtt assaysealer |
| spellingShingle | Salma Omidi Kosar Dadgar Abbas Mesgarani Mahmoud Moosazade Fatemeh Zaghi Hosseinzadeh Evaluation of Cytotoxicity of Endoseal MTA Sealer as a Root Canal Perforation Restorative Material on Gingival Fibroblasts Compared to Iranian ProRoot MTA and CEM by MTT test Journal of Mashhad Dental School cem cytotoxicity endoseal mta mtt assay sealer |
| title | Evaluation of Cytotoxicity of Endoseal MTA Sealer as a Root Canal Perforation Restorative Material on Gingival Fibroblasts Compared to Iranian ProRoot MTA and CEM by MTT test |
| title_full | Evaluation of Cytotoxicity of Endoseal MTA Sealer as a Root Canal Perforation Restorative Material on Gingival Fibroblasts Compared to Iranian ProRoot MTA and CEM by MTT test |
| title_fullStr | Evaluation of Cytotoxicity of Endoseal MTA Sealer as a Root Canal Perforation Restorative Material on Gingival Fibroblasts Compared to Iranian ProRoot MTA and CEM by MTT test |
| title_full_unstemmed | Evaluation of Cytotoxicity of Endoseal MTA Sealer as a Root Canal Perforation Restorative Material on Gingival Fibroblasts Compared to Iranian ProRoot MTA and CEM by MTT test |
| title_short | Evaluation of Cytotoxicity of Endoseal MTA Sealer as a Root Canal Perforation Restorative Material on Gingival Fibroblasts Compared to Iranian ProRoot MTA and CEM by MTT test |
| title_sort | evaluation of cytotoxicity of endoseal mta sealer as a root canal perforation restorative material on gingival fibroblasts compared to iranian proroot mta and cem by mtt test |
| topic | cem cytotoxicity endoseal mta mtt assay sealer |
| url | https://jmds.mums.ac.ir/article_21406_4f2fadcc6578b60d13b00b6e2547d1e3.pdf |
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