Detection of F94L marker in myostatin (MSTN/TaqI) gene of Indonesian Sumba Ongole cattle (Bos indicus)
Sumba Ongole (SO) cattle are Ongole cattle from India which were imported to Indonesia during the Dutch colonial era and were well adapted on Sumba Island, East Nusa Tenggara, Indonesia. Sumba Ongole (SO) cattle are known for high meat production. This research was conducted to identify one point m...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Indian Council of Agricultural Research
2022-09-01
|
| Series: | Indian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/121083 |
| _version_ | 1811169098815504384 |
|---|---|
| author | HARTATI W P B PUTRA B D P SOEWANDI S ANWAR S RATNAWATY |
| author_facet | HARTATI W P B PUTRA B D P SOEWANDI S ANWAR S RATNAWATY |
| author_sort | HARTATI |
| collection | DOAJ |
| description |
Sumba Ongole (SO) cattle are Ongole cattle from India which were imported to Indonesia during the Dutch colonial era and were well adapted on Sumba Island, East Nusa Tenggara, Indonesia. Sumba Ongole (SO) cattle are known for high meat production. This research was conducted to identify one point mutation of g.415C/A (F94L marker) in the myostatin (MSTN) gene (exon 1) using PCR-RFLP method with TaqI restriction enzyme (T*CGA). DNA samples of SO bulls and cows (Total 153) from Sumba Island were used in this study. Results revealed that all samples in this study had CC genotype with C allele as the common allele. The mutation point of g.415C/A was not found in this study. However, one mutation point of g.400G/A was detected in this study without amino acid changes (synonymous). It was concluded that MSTN/TaqI gene in the present study is monomorphic and cannot be used as the genetic marker for productive traits of SO cattle.
|
| first_indexed | 2024-04-10T16:36:40Z |
| format | Article |
| id | doaj.art-577e6e5e0f684b97a1de40317a29772d |
| institution | Directory Open Access Journal |
| issn | 0367-8318 2394-3327 |
| language | English |
| last_indexed | 2024-04-10T16:36:40Z |
| publishDate | 2022-09-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | Indian Journal of Animal Sciences |
| spelling | doaj.art-577e6e5e0f684b97a1de40317a29772d2023-02-08T11:22:59ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272022-09-0192910.56093/ijans.v92i9.121083Detection of F94L marker in myostatin (MSTN/TaqI) gene of Indonesian Sumba Ongole cattle (Bos indicus)HARTATI0W P B PUTRA1B D P SOEWANDI2S ANWAR3S RATNAWATY4National Research and Innovation Agency, Bogor 16915, IndonesiaResearch Center for Applied Zoology, National Research and Innovation Agency, Bogor 16911, IndonesiaNational Research and Innovation Agency, Bogor 16915, IndonesiaResearch Center for Applied Zoology, National Research and Innovation Agency, Bogor 16911, Indonesia.East Nusa Tenggara Assessment Institute for Agricultural Technology (IRIAP), Kupang–Indonesia. Sumba Ongole (SO) cattle are Ongole cattle from India which were imported to Indonesia during the Dutch colonial era and were well adapted on Sumba Island, East Nusa Tenggara, Indonesia. Sumba Ongole (SO) cattle are known for high meat production. This research was conducted to identify one point mutation of g.415C/A (F94L marker) in the myostatin (MSTN) gene (exon 1) using PCR-RFLP method with TaqI restriction enzyme (T*CGA). DNA samples of SO bulls and cows (Total 153) from Sumba Island were used in this study. Results revealed that all samples in this study had CC genotype with C allele as the common allele. The mutation point of g.415C/A was not found in this study. However, one mutation point of g.400G/A was detected in this study without amino acid changes (synonymous). It was concluded that MSTN/TaqI gene in the present study is monomorphic and cannot be used as the genetic marker for productive traits of SO cattle. https://epubs.icar.org.in/index.php/IJAnS/article/view/121083F94L marker, MSTN/TaqI gene, Sumba Ongole (SO) cattle |
| spellingShingle | HARTATI W P B PUTRA B D P SOEWANDI S ANWAR S RATNAWATY Detection of F94L marker in myostatin (MSTN/TaqI) gene of Indonesian Sumba Ongole cattle (Bos indicus) Indian Journal of Animal Sciences F94L marker, MSTN/TaqI gene, Sumba Ongole (SO) cattle |
| title | Detection of F94L marker in myostatin (MSTN/TaqI) gene of Indonesian Sumba Ongole cattle (Bos indicus) |
| title_full | Detection of F94L marker in myostatin (MSTN/TaqI) gene of Indonesian Sumba Ongole cattle (Bos indicus) |
| title_fullStr | Detection of F94L marker in myostatin (MSTN/TaqI) gene of Indonesian Sumba Ongole cattle (Bos indicus) |
| title_full_unstemmed | Detection of F94L marker in myostatin (MSTN/TaqI) gene of Indonesian Sumba Ongole cattle (Bos indicus) |
| title_short | Detection of F94L marker in myostatin (MSTN/TaqI) gene of Indonesian Sumba Ongole cattle (Bos indicus) |
| title_sort | detection of f94l marker in myostatin mstn taqi gene of indonesian sumba ongole cattle bos indicus |
| topic | F94L marker, MSTN/TaqI gene, Sumba Ongole (SO) cattle |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/121083 |
| work_keys_str_mv | AT hartati detectionoff94lmarkerinmyostatinmstntaqigeneofindonesiansumbaongolecattlebosindicus AT wpbputra detectionoff94lmarkerinmyostatinmstntaqigeneofindonesiansumbaongolecattlebosindicus AT bdpsoewandi detectionoff94lmarkerinmyostatinmstntaqigeneofindonesiansumbaongolecattlebosindicus AT sanwar detectionoff94lmarkerinmyostatinmstntaqigeneofindonesiansumbaongolecattlebosindicus AT sratnawaty detectionoff94lmarkerinmyostatinmstntaqigeneofindonesiansumbaongolecattlebosindicus |