Interplay between Fungal Infection and Bacterial Associates in the Wax Moth <i>Galleria mellonella</i> under Different Temperature Conditions
Various insect bacterial associates are involved in pathogeneses caused by entomopathogenic fungi. The outcome of infection (fungal growth or decomposition) may depend on environmental factors such as temperature. The aim of this study was to analyze the bacterial communities and immune response of...
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MDPI AG
2020-09-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/6/3/170 |
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| author | Vadim Yu Kryukov Elena Kosman Oksana Tomilova Olga Polenogova Ulyana Rotskaya Maksim Tyurin Tatyana Alikina Olga Yaroslavtseva Marsel Kabilov Viktor Glupov |
| author_facet | Vadim Yu Kryukov Elena Kosman Oksana Tomilova Olga Polenogova Ulyana Rotskaya Maksim Tyurin Tatyana Alikina Olga Yaroslavtseva Marsel Kabilov Viktor Glupov |
| author_sort | Vadim Yu Kryukov |
| collection | DOAJ |
| description | Various insect bacterial associates are involved in pathogeneses caused by entomopathogenic fungi. The outcome of infection (fungal growth or decomposition) may depend on environmental factors such as temperature. The aim of this study was to analyze the bacterial communities and immune response of <i>Galleria mellonella</i> larvae injected with <i>Cordyceps militaris</i> and incubated at 15 °C and 25 °C. We examined changes in the bacterial CFUs, bacterial communities (Illumina MiSeq 16S rRNA gene sequencing) and expression of immune, apoptosis, ROS and stress-related genes (qPCR) in larval tissues in response to fungal infection at the mentioned temperatures. Increased survival of larvae after <i>C. militaris</i> injection was observed at 25 °C, although more frequent episodes of spontaneous bacteriosis were observed at this temperature compared to 15 °C. We revealed an increase in the abundance of enterococci and enterobacteria in the midgut and hemolymph in response to infection at 25 °C, which was not observed at 15 °C. Antifungal peptide genes showed the highest expression at 25 °C, while antibacterial peptides and inhibitor of apoptosis genes were strongly expressed at 15 °C. Cultivable bacteria significantly suppressed the growth of <i>C. militaris</i>. We suggest that fungi such as <i>C. militaris</i> may need low temperatures to avoid competition with host bacterial associates. |
| first_indexed | 2024-03-10T16:25:25Z |
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| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-10T16:25:25Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-577eadffcf2143088ddeb2b4907a16662023-11-20T13:18:49ZengMDPI AGJournal of Fungi2309-608X2020-09-016317010.3390/jof6030170Interplay between Fungal Infection and Bacterial Associates in the Wax Moth <i>Galleria mellonella</i> under Different Temperature ConditionsVadim Yu Kryukov0Elena Kosman1Oksana Tomilova2Olga Polenogova3Ulyana Rotskaya4Maksim Tyurin5Tatyana Alikina6Olga Yaroslavtseva7Marsel Kabilov8Viktor Glupov9Institute of Systematics and Ecology of Animals SB RAS, Frunze str. 11, 630091 Novosibirsk, RussiaInstitute of Systematics and Ecology of Animals SB RAS, Frunze str. 11, 630091 Novosibirsk, RussiaInstitute of Systematics and Ecology of Animals SB RAS, Frunze str. 11, 630091 Novosibirsk, RussiaInstitute of Systematics and Ecology of Animals SB RAS, Frunze str. 11, 630091 Novosibirsk, RussiaInstitute of Systematics and Ecology of Animals SB RAS, Frunze str. 11, 630091 Novosibirsk, RussiaInstitute of Systematics and Ecology of Animals SB RAS, Frunze str. 11, 630091 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine SB RAS, Lavrentiev av. 8, 630090 Novosibirsk, RussiaInstitute of Systematics and Ecology of Animals SB RAS, Frunze str. 11, 630091 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine SB RAS, Lavrentiev av. 8, 630090 Novosibirsk, RussiaInstitute of Systematics and Ecology of Animals SB RAS, Frunze str. 11, 630091 Novosibirsk, RussiaVarious insect bacterial associates are involved in pathogeneses caused by entomopathogenic fungi. The outcome of infection (fungal growth or decomposition) may depend on environmental factors such as temperature. The aim of this study was to analyze the bacterial communities and immune response of <i>Galleria mellonella</i> larvae injected with <i>Cordyceps militaris</i> and incubated at 15 °C and 25 °C. We examined changes in the bacterial CFUs, bacterial communities (Illumina MiSeq 16S rRNA gene sequencing) and expression of immune, apoptosis, ROS and stress-related genes (qPCR) in larval tissues in response to fungal infection at the mentioned temperatures. Increased survival of larvae after <i>C. militaris</i> injection was observed at 25 °C, although more frequent episodes of spontaneous bacteriosis were observed at this temperature compared to 15 °C. We revealed an increase in the abundance of enterococci and enterobacteria in the midgut and hemolymph in response to infection at 25 °C, which was not observed at 15 °C. Antifungal peptide genes showed the highest expression at 25 °C, while antibacterial peptides and inhibitor of apoptosis genes were strongly expressed at 15 °C. Cultivable bacteria significantly suppressed the growth of <i>C. militaris</i>. We suggest that fungi such as <i>C. militaris</i> may need low temperatures to avoid competition with host bacterial associates.https://www.mdpi.com/2309-608X/6/3/170insectsmycosesspontaneous bacteriosesfungal–bacteria interactions<i>Cordyceps militaris</i>antimicrobial peptides |
| spellingShingle | Vadim Yu Kryukov Elena Kosman Oksana Tomilova Olga Polenogova Ulyana Rotskaya Maksim Tyurin Tatyana Alikina Olga Yaroslavtseva Marsel Kabilov Viktor Glupov Interplay between Fungal Infection and Bacterial Associates in the Wax Moth <i>Galleria mellonella</i> under Different Temperature Conditions Journal of Fungi insects mycoses spontaneous bacterioses fungal–bacteria interactions <i>Cordyceps militaris</i> antimicrobial peptides |
| title | Interplay between Fungal Infection and Bacterial Associates in the Wax Moth <i>Galleria mellonella</i> under Different Temperature Conditions |
| title_full | Interplay between Fungal Infection and Bacterial Associates in the Wax Moth <i>Galleria mellonella</i> under Different Temperature Conditions |
| title_fullStr | Interplay between Fungal Infection and Bacterial Associates in the Wax Moth <i>Galleria mellonella</i> under Different Temperature Conditions |
| title_full_unstemmed | Interplay between Fungal Infection and Bacterial Associates in the Wax Moth <i>Galleria mellonella</i> under Different Temperature Conditions |
| title_short | Interplay between Fungal Infection and Bacterial Associates in the Wax Moth <i>Galleria mellonella</i> under Different Temperature Conditions |
| title_sort | interplay between fungal infection and bacterial associates in the wax moth i galleria mellonella i under different temperature conditions |
| topic | insects mycoses spontaneous bacterioses fungal–bacteria interactions <i>Cordyceps militaris</i> antimicrobial peptides |
| url | https://www.mdpi.com/2309-608X/6/3/170 |
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