Microwell scaffolds for the extrahepatic transplantation of islets of Langerhans.
Allogeneic islet transplantation into the liver has the potential to restore normoglycemia in patients with type 1 diabetes. However, the suboptimal microenvironment for islets in the liver is likely to be involved in the progressive islet dysfunction that is often observed post-transplantation. Thi...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3667808?pdf=render |
| _version_ | 1818791279746613248 |
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| author | Mijke Buitinga Roman Truckenmüller Marten A Engelse Lorenzo Moroni Hetty W M Ten Hoopen Clemens A van Blitterswijk Eelco Jp de Koning Aart A van Apeldoorn Marcel Karperien |
| author_facet | Mijke Buitinga Roman Truckenmüller Marten A Engelse Lorenzo Moroni Hetty W M Ten Hoopen Clemens A van Blitterswijk Eelco Jp de Koning Aart A van Apeldoorn Marcel Karperien |
| author_sort | Mijke Buitinga |
| collection | DOAJ |
| description | Allogeneic islet transplantation into the liver has the potential to restore normoglycemia in patients with type 1 diabetes. However, the suboptimal microenvironment for islets in the liver is likely to be involved in the progressive islet dysfunction that is often observed post-transplantation. This study validates a novel microwell scaffold platform to be used for the extrahepatic transplantation of islet of Langerhans. Scaffolds were fabricated from either a thin polymer film or an electrospun mesh of poly(ethylene oxide terephthalate)-poly(butylene terephthalate) (PEOT/PBT) block copolymer (composition: 4000PEOT30PBT70) and were imprinted with microwells, ∼400 µm in diameter and ∼350 µm in depth. The water contact angle and water uptake were 39±2° and 52.1±4.0 wt%, respectively. The glucose flux through electrospun scaffolds was three times higher than for thin film scaffolds, indicating enhanced nutrient diffusion. Human islets cultured in microwell scaffolds for seven days showed insulin release and insulin content comparable to those of free-floating control islets. Islet morphology and insulin and glucagon expression were maintained during culture in the microwell scaffolds. Our results indicate that the microwell scaffold platform prevents islet aggregation by confinement of individual islets in separate microwells, preserves the islet's native rounded morphology, and provides a protective environment without impairing islet functionality, making it a promising platform for use in extrahepatic islet transplantation. |
| first_indexed | 2024-12-18T15:08:50Z |
| format | Article |
| id | doaj.art-5780d1365e654eb4b630dcc82e4c7b35 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-18T15:08:50Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-5780d1365e654eb4b630dcc82e4c7b352022-12-21T21:03:42ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0185e6477210.1371/journal.pone.0064772Microwell scaffolds for the extrahepatic transplantation of islets of Langerhans.Mijke BuitingaRoman TruckenmüllerMarten A EngelseLorenzo MoroniHetty W M Ten HoopenClemens A van BlitterswijkEelco Jp de KoningAart A van ApeldoornMarcel KarperienAllogeneic islet transplantation into the liver has the potential to restore normoglycemia in patients with type 1 diabetes. However, the suboptimal microenvironment for islets in the liver is likely to be involved in the progressive islet dysfunction that is often observed post-transplantation. This study validates a novel microwell scaffold platform to be used for the extrahepatic transplantation of islet of Langerhans. Scaffolds were fabricated from either a thin polymer film or an electrospun mesh of poly(ethylene oxide terephthalate)-poly(butylene terephthalate) (PEOT/PBT) block copolymer (composition: 4000PEOT30PBT70) and were imprinted with microwells, ∼400 µm in diameter and ∼350 µm in depth. The water contact angle and water uptake were 39±2° and 52.1±4.0 wt%, respectively. The glucose flux through electrospun scaffolds was three times higher than for thin film scaffolds, indicating enhanced nutrient diffusion. Human islets cultured in microwell scaffolds for seven days showed insulin release and insulin content comparable to those of free-floating control islets. Islet morphology and insulin and glucagon expression were maintained during culture in the microwell scaffolds. Our results indicate that the microwell scaffold platform prevents islet aggregation by confinement of individual islets in separate microwells, preserves the islet's native rounded morphology, and provides a protective environment without impairing islet functionality, making it a promising platform for use in extrahepatic islet transplantation.http://europepmc.org/articles/PMC3667808?pdf=render |
| spellingShingle | Mijke Buitinga Roman Truckenmüller Marten A Engelse Lorenzo Moroni Hetty W M Ten Hoopen Clemens A van Blitterswijk Eelco Jp de Koning Aart A van Apeldoorn Marcel Karperien Microwell scaffolds for the extrahepatic transplantation of islets of Langerhans. PLoS ONE |
| title | Microwell scaffolds for the extrahepatic transplantation of islets of Langerhans. |
| title_full | Microwell scaffolds for the extrahepatic transplantation of islets of Langerhans. |
| title_fullStr | Microwell scaffolds for the extrahepatic transplantation of islets of Langerhans. |
| title_full_unstemmed | Microwell scaffolds for the extrahepatic transplantation of islets of Langerhans. |
| title_short | Microwell scaffolds for the extrahepatic transplantation of islets of Langerhans. |
| title_sort | microwell scaffolds for the extrahepatic transplantation of islets of langerhans |
| url | http://europepmc.org/articles/PMC3667808?pdf=render |
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