| Summary: | Bioaerosol, particulate matter concentration and antibiotic resistance of airborne <i>Staphylococcus</i> was assessed in animal and public premises (classroom, sports hall, horse stable, cowshed, newborn calf shed and outdoor background control site) of Poland’s oldest agricultural school. The concentration and size distribution of bacteria, fungi, actinomycetes and staphylococci were assessed with a six stage Andersen impactor. Particulate matter (PM<sub>10</sub>, PM<sub>4</sub>, PM<sub>2.5</sub> and PM<sub>1</sub>) was determined using the DustTrak aerosol monitor. The <i>Staphylococcus</i> species were determined with MALDI-TOF mass spectrometry and antimicrobial resistance was assessed using disk diffusion. Bioaerosol concentrations differed significantly between sampling points, with the highest levels of all microorganisms occurring in the newborn calf shed. The proportion of respirable fraction exceeded 60% in all sites, indicating potential harmfulness to exposed people. Mean concentrations of particulate matter were the smallest in school rooms and the highest in the newborn calf shed. Neither particulate matter nor microbial aerosol exceeded threshold values for workplaces. Among thirty-four isolated staphylococcal strains, <i>S. equorum</i> (35%), <i>S. succinus</i> (26%) and <i>S. xylosus</i> (15%) were the most prevalent. Resistance to macrolides (erythromycin) and lincosamides (clindamycin) was the most frequent. One strain was methicillin-resistant. Farm animals are significant sources of bioaerosol and therefore attention should be paid with respect to maintaining appropriate sanitary conditions and hygiene of premises and animals.
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