Development of an intraoperative breast cancer margin assessment method using quantitative fluorescence measurements
Abstract Breast-conserving surgery has become the preferred treatment method for breast cancer. Surgical margin assessment is performed during surgery, as it can reduce local recurrence in the preserved breast. Development of reliable and lower-cost ex vivo cancer detection methods would offer sever...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-12614-6 |
| _version_ | 1818240736541278208 |
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| author | Hiroki Ueo Itsushi Minoura Hiroaki Ueo Ayako Gamachi Yuichiro Kai Yoko Kubota Takako Doi Miki Yamaguchi Toshinari Yamashita Hitoshi Tsuda Takuya Moriya Rin Yamaguchi Yuji Kozuka Takeshi Sasaki Takaaki Masuda Yasuteru Urano Masaki Mori Koshi Mimori |
| author_facet | Hiroki Ueo Itsushi Minoura Hiroaki Ueo Ayako Gamachi Yuichiro Kai Yoko Kubota Takako Doi Miki Yamaguchi Toshinari Yamashita Hitoshi Tsuda Takuya Moriya Rin Yamaguchi Yuji Kozuka Takeshi Sasaki Takaaki Masuda Yasuteru Urano Masaki Mori Koshi Mimori |
| author_sort | Hiroki Ueo |
| collection | DOAJ |
| description | Abstract Breast-conserving surgery has become the preferred treatment method for breast cancer. Surgical margin assessment is performed during surgery, as it can reduce local recurrence in the preserved breast. Development of reliable and lower-cost ex vivo cancer detection methods would offer several benefits for patient care. Here, a practical and quantitative evaluation method for the ex vivo fluorescent diagnosis of breast lesions was developed and confirmed through a three-step clinical study. Gamma-glutamyl-hydroxymethyl rhodamine green (gGlu-HMRG) has been reported to generate fluorescence in breast lesions. Using this probe, we constructed a reliable and reproducible procedure for the quantitative evaluation of fluorescence levels. We evaluated the reliability of the method by considering reproducibility, temperature sensitivity, and the effects of other clinicopathological factors. The results suggest that the fluorescence increase of gGlu-HMRG is a good indicator of the malignancy of breast lesions. However, the distributions overlapped. A 5 min reaction with this probe could be used to distinguish at least part of the normal breast tissue. This method did not affect the final pathological examination. In summary, our results indicate that the methods developed in this study may serve as a feasible intraoperative negative-margin assessment tool during breast-conserving surgery. |
| first_indexed | 2024-12-12T13:18:11Z |
| format | Article |
| id | doaj.art-57841e403e1d428fb43fed7ca65cece2 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-12T13:18:11Z |
| publishDate | 2022-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-57841e403e1d428fb43fed7ca65cece22022-12-22T00:23:22ZengNature PortfolioScientific Reports2045-23222022-05-0112111010.1038/s41598-022-12614-6Development of an intraoperative breast cancer margin assessment method using quantitative fluorescence measurementsHiroki Ueo0Itsushi Minoura1Hiroaki Ueo2Ayako Gamachi3Yuichiro Kai4Yoko Kubota5Takako Doi6Miki Yamaguchi7Toshinari Yamashita8Hitoshi Tsuda9Takuya Moriya10Rin Yamaguchi11Yuji Kozuka12Takeshi Sasaki13Takaaki Masuda14Yasuteru Urano15Masaki Mori16Koshi Mimori17Ueo Breast Cancer HospitalGoryo Chemical Inc.Ueo Breast Cancer HospitalDepartment of Pathology, Almeida Memorial HospitalUeo Breast Cancer HospitalUeo Breast Cancer HospitalBreast Cancer Center, Shonan Memorial HospitalDepartment of Breast Surgery, JCHO Kurume General HospitalDepartment of Breast and Endocrine Surgery, Kanagawa Cancer CenterDepartment of Basic Pathology, National Defense Medical CollegeDepartment of Pathology, Kawasaki Medical SchoolDepartment of Pathology and Laboratory Medicine, Kurume University Medical CenterDepartment of Pathology, Mie University HospitalDepartment of Next-Generation Pathology Information and Networking, Faculty of Medicine, The University of TokyoDepartment of Surgery, Kyushu University Beppu HospitalGraduate School of Medicine and Graduate School of Pharmaceutical Sciences, The University of TokyoDepartment of Surgery and Sciences, Graduate School of Medical Sciences, Kyushu UniversityDepartment of Surgery, Kyushu University Beppu HospitalAbstract Breast-conserving surgery has become the preferred treatment method for breast cancer. Surgical margin assessment is performed during surgery, as it can reduce local recurrence in the preserved breast. Development of reliable and lower-cost ex vivo cancer detection methods would offer several benefits for patient care. Here, a practical and quantitative evaluation method for the ex vivo fluorescent diagnosis of breast lesions was developed and confirmed through a three-step clinical study. Gamma-glutamyl-hydroxymethyl rhodamine green (gGlu-HMRG) has been reported to generate fluorescence in breast lesions. Using this probe, we constructed a reliable and reproducible procedure for the quantitative evaluation of fluorescence levels. We evaluated the reliability of the method by considering reproducibility, temperature sensitivity, and the effects of other clinicopathological factors. The results suggest that the fluorescence increase of gGlu-HMRG is a good indicator of the malignancy of breast lesions. However, the distributions overlapped. A 5 min reaction with this probe could be used to distinguish at least part of the normal breast tissue. This method did not affect the final pathological examination. In summary, our results indicate that the methods developed in this study may serve as a feasible intraoperative negative-margin assessment tool during breast-conserving surgery.https://doi.org/10.1038/s41598-022-12614-6 |
| spellingShingle | Hiroki Ueo Itsushi Minoura Hiroaki Ueo Ayako Gamachi Yuichiro Kai Yoko Kubota Takako Doi Miki Yamaguchi Toshinari Yamashita Hitoshi Tsuda Takuya Moriya Rin Yamaguchi Yuji Kozuka Takeshi Sasaki Takaaki Masuda Yasuteru Urano Masaki Mori Koshi Mimori Development of an intraoperative breast cancer margin assessment method using quantitative fluorescence measurements Scientific Reports |
| title | Development of an intraoperative breast cancer margin assessment method using quantitative fluorescence measurements |
| title_full | Development of an intraoperative breast cancer margin assessment method using quantitative fluorescence measurements |
| title_fullStr | Development of an intraoperative breast cancer margin assessment method using quantitative fluorescence measurements |
| title_full_unstemmed | Development of an intraoperative breast cancer margin assessment method using quantitative fluorescence measurements |
| title_short | Development of an intraoperative breast cancer margin assessment method using quantitative fluorescence measurements |
| title_sort | development of an intraoperative breast cancer margin assessment method using quantitative fluorescence measurements |
| url | https://doi.org/10.1038/s41598-022-12614-6 |
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