Fine-scale evaluation of two standard 16S rRNA gene amplicon primer pairs for analysis of total prokaryotes and archaeal nitrifiers in differently managed soils

Fine-scale evaluation of two standard 16S rRNA gene amplicon primer pairs for analysis of total prokaryotes and archaeal nitrifiers in differently managed soils

The advance of high-throughput molecular biology tools allows in-depth profiling of microbial communities in soils, which possess a high diversity of prokaryotic microorganisms. Amplicon-based sequencing of 16S rRNA genes is the most common approach to studying the richness and composition of soil p...

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Bibliographic Details
Main Authors:	Jun Zhao, Jonathan Rodriguez, Willm Martens-Habbena
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2023-02-01
Series:	Frontiers in Microbiology
Subjects:	16S rRNA gene amplicon Illumina sequencing metagenomics soil ammonia-oxidizing archaea primer evaluation microbial communities
Online Access:	https://www.frontiersin.org/articles/10.3389/fmicb.2023.1140487/full

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