Comparative study of HbA1c measurement using EDTA blood vs. NaF blood
HbA1c is a glycated hemoglobin which was used to assess the glucose level of diabetes mellitus patients for the previous 1-2 month. The standard method for the measurement of HbA1c requires EDTA blood. Almost all physicians requesting HbA1c also include a blood glucose test in the same requisition t...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Prince of Songkla University
2005-04-01
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| Series: | Journal of Health Science and Medical Research (JHSMR) |
| Subjects: | |
| Online Access: | https://www.jhsmr.org/index.php/jhsmr/article/view/508 |
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| author | P Chusongsang P Musigawan N Wannapong A Musow P Apirakthunyakorn |
| author_facet | P Chusongsang P Musigawan N Wannapong A Musow P Apirakthunyakorn |
| author_sort | P Chusongsang |
| collection | DOAJ |
| description | HbA1c is a glycated hemoglobin which was used to assess the glucose level of diabetes mellitus patients for the previous 1-2 month. The standard method for the measurement of HbA1c requires EDTA blood. Almost all physicians requesting HbA1c also include a blood glucose test in the same requisition test panels that requires a NaF blood sample. From these requisition test panels, an absence of either an EDTA blood or NaF blood sample to complete the requirement for the determination of both HbA1c and blood glucose is common. Thus, the main aim of this research is to attempt to introduce the use of NaF blood in order to determine the amount of both HbA1c and glucose. A comparison of the HbA1c values obtained from using the EDTA blood and the NaF blood was established. A total of 212 patient blood samples was collected and both EDTA blood and NaF blood were analyzed by the Hitachi 717 automatic analyzer using the Turbid metric Inhibition Immunoassay method (Kit reagent from Roche Diagnostic, Thailand).The results from this study, the mean value of HbA1c obtained from EDTA blood and NaF blood samples were 8.293 ± 2.076 and 8.265 ± 2.086 respectively. There was no sig
nificant difference between the HbA1c value obtained from EDTA blood and NaF blood (p > 0.05), mean difference 0.028 and regression equation y = 0.999x-0.019, r = 0.994. In conclusion, the value of HbA1c measured from NaF blood samples was almost equal value with that of the EDTA blood sample. Therefore, at Songklanagarind Hospital, the NaF blood sample can be used to determine HbA1c as well as EDTA blood. |
| first_indexed | 2024-12-11T02:21:57Z |
| format | Article |
| id | doaj.art-57892b28c734491da3d96715197b05c4 |
| institution | Directory Open Access Journal |
| issn | 2586-9981 2630-0559 |
| language | English |
| last_indexed | 2024-12-11T02:21:57Z |
| publishDate | 2005-04-01 |
| publisher | Prince of Songkla University |
| record_format | Article |
| series | Journal of Health Science and Medical Research (JHSMR) |
| spelling | doaj.art-57892b28c734491da3d96715197b05c42022-12-22T01:24:02ZengPrince of Songkla UniversityJournal of Health Science and Medical Research (JHSMR)2586-99812630-05592005-04-012327379522Comparative study of HbA1c measurement using EDTA blood vs. NaF bloodP Chusongsang0P Musigawan1N Wannapong2A Musow3P Apirakthunyakorn4Chemistry Unit, Department of Pathology, Faculty of Medicine,Prince of Songkla University, Hat Yai, Songkhla 90110,Chemistry Unit, Department of Pathology, Faculty of Medicine,Prince of Songkla University, Hat Yai, Songkhla 90110,Chemistry Unit, Department of Pathology, Faculty of Medicine,Prince of Songkla University, Hat Yai, Songkhla 90110,Chemistry Unit, Department of Pathology, Faculty of Medicine,Prince of Songkla University, Hat Yai, Songkhla 90110,Chemistry Unit, Department of Pathology, Faculty of Medicine,Prince of Songkla University, Hat Yai, Songkhla 90110,HbA1c is a glycated hemoglobin which was used to assess the glucose level of diabetes mellitus patients for the previous 1-2 month. The standard method for the measurement of HbA1c requires EDTA blood. Almost all physicians requesting HbA1c also include a blood glucose test in the same requisition test panels that requires a NaF blood sample. From these requisition test panels, an absence of either an EDTA blood or NaF blood sample to complete the requirement for the determination of both HbA1c and blood glucose is common. Thus, the main aim of this research is to attempt to introduce the use of NaF blood in order to determine the amount of both HbA1c and glucose. A comparison of the HbA1c values obtained from using the EDTA blood and the NaF blood was established. A total of 212 patient blood samples was collected and both EDTA blood and NaF blood were analyzed by the Hitachi 717 automatic analyzer using the Turbid metric Inhibition Immunoassay method (Kit reagent from Roche Diagnostic, Thailand).The results from this study, the mean value of HbA1c obtained from EDTA blood and NaF blood samples were 8.293 ± 2.076 and 8.265 ± 2.086 respectively. There was no sig nificant difference between the HbA1c value obtained from EDTA blood and NaF blood (p > 0.05), mean difference 0.028 and regression equation y = 0.999x-0.019, r = 0.994. In conclusion, the value of HbA1c measured from NaF blood samples was almost equal value with that of the EDTA blood sample. Therefore, at Songklanagarind Hospital, the NaF blood sample can be used to determine HbA1c as well as EDTA blood.https://www.jhsmr.org/index.php/jhsmr/article/view/508hba1c, diabetes mellitus, edta blood, naf blood, immunoassay |
| spellingShingle | P Chusongsang P Musigawan N Wannapong A Musow P Apirakthunyakorn Comparative study of HbA1c measurement using EDTA blood vs. NaF blood Journal of Health Science and Medical Research (JHSMR) hba1c, diabetes mellitus, edta blood, naf blood, immunoassay |
| title | Comparative study of HbA1c measurement using EDTA blood vs. NaF blood |
| title_full | Comparative study of HbA1c measurement using EDTA blood vs. NaF blood |
| title_fullStr | Comparative study of HbA1c measurement using EDTA blood vs. NaF blood |
| title_full_unstemmed | Comparative study of HbA1c measurement using EDTA blood vs. NaF blood |
| title_short | Comparative study of HbA1c measurement using EDTA blood vs. NaF blood |
| title_sort | comparative study of hba1c measurement using edta blood vs naf blood |
| topic | hba1c, diabetes mellitus, edta blood, naf blood, immunoassay |
| url | https://www.jhsmr.org/index.php/jhsmr/article/view/508 |
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