Use of droplet digital PCR for estimation of fish abundance and biomass in environmental DNA surveys.
An environmental DNA (eDNA) analysis method has been recently developed to estimate the distribution of aquatic animals by quantifying the number of target DNA copies with quantitative real-time PCR (qPCR). A new quantitative PCR technology, droplet digital PCR (ddPCR), partitions PCR reactions into...
Main Authors: | Hideyuki Doi, Kimiko Uchii, Teruhiko Takahara, Saeko Matsuhashi, Hiroki Yamanaka, Toshifumi Minamoto |
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Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2015-01-01
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Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC4370432?pdf=render |
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