The capsule polysaccharide structure and biogenesis for non-O1 Vibrio cholerae NRT36S: genes are embedded in the LPS region
<p>Abstract</p> <p>Background</p> <p>In <it>V. cholerae</it>, the biogenesis of capsule polysaccharide is poorly understood. The elucidation of capsule structure and biogenesis is critical to understanding the evolution of surface polysaccharide and the inte...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2007-03-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/7/20 |
| _version_ | 1818473035843239936 |
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| author | Johnson Judith A Ali Afsar Panigrahi Pinaki Adeyeye Jacob Bystricky Peter Chen Yuansha Bush CA Morris JG Stine OC |
| author_facet | Johnson Judith A Ali Afsar Panigrahi Pinaki Adeyeye Jacob Bystricky Peter Chen Yuansha Bush CA Morris JG Stine OC |
| author_sort | Johnson Judith A |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>In <it>V. cholerae</it>, the biogenesis of capsule polysaccharide is poorly understood. The elucidation of capsule structure and biogenesis is critical to understanding the evolution of surface polysaccharide and the internal relationship between the capsule and LPS in this species. <it>V. cholerae </it>serogroup O31 NRT36S, a human pathogen that produces a heat-stable enterotoxin (NAG-ST), is encapsulated. Here, we report the covalent structure and studies of the biogenesis of the capsule in <it>V. cholerae </it>NRT36S.</p> <p>Results</p> <p>The structure of the capsular (CPS) polysaccharide was determined by high resolution NMR spectroscopy and shown to be a complex structure with four residues in the repeating subunit. The gene cluster of capsule biogenesis was identified by transposon mutagenesis combined with whole genome sequencing data (GenBank accession DQ915177). The capsule gene cluster shared the same genetic locus as that of the O-antigen of lipopolysaccharide (LPS) biogenesis gene cluster. Other than <it>V. cholerae </it>O139, this is the first <it>V. cholerae </it>CPS for which a structure has been fully elucidated and the genetic locus responsible for biosynthesis identified.</p> <p>Conclusion</p> <p>The co-location of CPS and LPS biosynthesis genes was unexpected, and would provide a mechanism for simultaneous emergence of new O and K antigens in a single strain. This, in turn, may be a key element for <it>V. cholerae </it>to evolve new strains that can escape immunologic detection by host populations.</p> |
| first_indexed | 2024-04-14T04:16:13Z |
| format | Article |
| id | doaj.art-578bba7c545743b19f00aa8a15360363 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-04-14T04:16:13Z |
| publishDate | 2007-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-578bba7c545743b19f00aa8a153603632022-12-22T02:12:48ZengBMCBMC Microbiology1471-21802007-03-01712010.1186/1471-2180-7-20The capsule polysaccharide structure and biogenesis for non-O1 Vibrio cholerae NRT36S: genes are embedded in the LPS regionJohnson Judith AAli AfsarPanigrahi PinakiAdeyeye JacobBystricky PeterChen YuanshaBush CAMorris JGStine OC<p>Abstract</p> <p>Background</p> <p>In <it>V. cholerae</it>, the biogenesis of capsule polysaccharide is poorly understood. The elucidation of capsule structure and biogenesis is critical to understanding the evolution of surface polysaccharide and the internal relationship between the capsule and LPS in this species. <it>V. cholerae </it>serogroup O31 NRT36S, a human pathogen that produces a heat-stable enterotoxin (NAG-ST), is encapsulated. Here, we report the covalent structure and studies of the biogenesis of the capsule in <it>V. cholerae </it>NRT36S.</p> <p>Results</p> <p>The structure of the capsular (CPS) polysaccharide was determined by high resolution NMR spectroscopy and shown to be a complex structure with four residues in the repeating subunit. The gene cluster of capsule biogenesis was identified by transposon mutagenesis combined with whole genome sequencing data (GenBank accession DQ915177). The capsule gene cluster shared the same genetic locus as that of the O-antigen of lipopolysaccharide (LPS) biogenesis gene cluster. Other than <it>V. cholerae </it>O139, this is the first <it>V. cholerae </it>CPS for which a structure has been fully elucidated and the genetic locus responsible for biosynthesis identified.</p> <p>Conclusion</p> <p>The co-location of CPS and LPS biosynthesis genes was unexpected, and would provide a mechanism for simultaneous emergence of new O and K antigens in a single strain. This, in turn, may be a key element for <it>V. cholerae </it>to evolve new strains that can escape immunologic detection by host populations.</p>http://www.biomedcentral.com/1471-2180/7/20 |
| spellingShingle | Johnson Judith A Ali Afsar Panigrahi Pinaki Adeyeye Jacob Bystricky Peter Chen Yuansha Bush CA Morris JG Stine OC The capsule polysaccharide structure and biogenesis for non-O1 Vibrio cholerae NRT36S: genes are embedded in the LPS region BMC Microbiology |
| title | The capsule polysaccharide structure and biogenesis for non-O1 Vibrio cholerae NRT36S: genes are embedded in the LPS region |
| title_full | The capsule polysaccharide structure and biogenesis for non-O1 Vibrio cholerae NRT36S: genes are embedded in the LPS region |
| title_fullStr | The capsule polysaccharide structure and biogenesis for non-O1 Vibrio cholerae NRT36S: genes are embedded in the LPS region |
| title_full_unstemmed | The capsule polysaccharide structure and biogenesis for non-O1 Vibrio cholerae NRT36S: genes are embedded in the LPS region |
| title_short | The capsule polysaccharide structure and biogenesis for non-O1 Vibrio cholerae NRT36S: genes are embedded in the LPS region |
| title_sort | capsule polysaccharide structure and biogenesis for non o1 vibrio cholerae nrt36s genes are embedded in the lps region |
| url | http://www.biomedcentral.com/1471-2180/7/20 |
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