Orthotopic T-cell receptor replacement in primary human T cells using CRISPR-Cas9-mediated homology-directed repair

Orthotopic T-cell receptor replacement in primary human T cells using CRISPR-Cas9-mediated homology-directed repair

Summary: Adoptive T cell therapy using T-cell receptor (TCR)-engineered T cells allows to redirect T cell specificity and to target any antigen of interest. Here, we apply advanced genetic engineering using clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (Cas9...

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Bibliographic Details
Main Authors: Carolin Moosmann, Thomas R. Müller, Dirk H. Busch, Kilian Schober
Format: Article
Language:English
Published: Elsevier 2022-03-01
Series:STAR Protocols
Subjects:
Health Sciences
Immunology
CRISPR
Biotechnology and bioengineering
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721007371

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