Loureirin B Inhibits Hypertrophic Scar Formation via Inhibition of the TGF-β1-ERK/JNK Pathway
Background/Aims: Our previous study confirmed that Loureirin B (LB) can inhibit hypertrophic scar formation. However, the mechanism of LB-mediated inhibition of scar formation is still unknown. Methods: Immunohistochemistry was used to detect expression of Col1, FN and TGF-β1 in skin and scar tissue...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Cell Physiol Biochem Press GmbH & Co KG
2015-09-01
|
| Series: | Cellular Physiology and Biochemistry |
| Subjects: | |
| Online Access: | http://www.karger.com/Article/FullText/430385 |
| _version_ | 1818936074532028416 |
|---|---|
| author | Ting He Xiaozhi Bai Longlong Yang Lei Fan Yan Li Linlin Su Jianxin Gao Shichao Han Dahai Hu |
| author_facet | Ting He Xiaozhi Bai Longlong Yang Lei Fan Yan Li Linlin Su Jianxin Gao Shichao Han Dahai Hu |
| author_sort | Ting He |
| collection | DOAJ |
| description | Background/Aims: Our previous study confirmed that Loureirin B (LB) can inhibit hypertrophic scar formation. However, the mechanism of LB-mediated inhibition of scar formation is still unknown. Methods: Immunohistochemistry was used to detect expression of Col1, FN and TGF-β1 in skin and scar tissue. Fibroblasts were stimulated with TGF-β1 to mimic scar formation. LB or MAPK inhibitors were used to study the pathways involved in the process. Western blotting was used to evaluate the expression of p-JNK, p-ERK, p-p38, Col1 and FN. The contractile capacity of fibroblasts was evaluated using a gel contraction assay. Tissues were cultured ex vivo with LB to further investigate the participation of ERK and JNK in the LB-mediated inhibition of scar formation. Results: FN and Col1 were up regulated in hypertrophic scars. LB down regulated p-ERK and p-JNK in TGF-β1-stimulated fibroblasts, while levels of phosphorylated p38 did not change. The down regulation of p-ERK and p-JNK was associated with a reduction of Col1 and FN. Similarly, inhibition of ERK and JNK down regulated the expression of Col1 and FN in TGF-β1-stimulated fibroblasts. LB down regulated protein levels of p-ERK and p-JNK in cultured hypertrophic scar tissue ex vivo. Conclusions: This study suggests that LB can inhibit scar formation through the ERK/JNK pathway. |
| first_indexed | 2024-12-20T05:30:17Z |
| format | Article |
| id | doaj.art-578f0940201147e38f2c0ea059931ad2 |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-12-20T05:30:17Z |
| publishDate | 2015-09-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-578f0940201147e38f2c0ea059931ad22022-12-21T19:51:45ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782015-09-0137266667610.1159/000430385430385Loureirin B Inhibits Hypertrophic Scar Formation via Inhibition of the TGF-β1-ERK/JNK PathwayTing HeXiaozhi BaiLonglong YangLei FanYan LiLinlin SuJianxin GaoShichao HanDahai HuBackground/Aims: Our previous study confirmed that Loureirin B (LB) can inhibit hypertrophic scar formation. However, the mechanism of LB-mediated inhibition of scar formation is still unknown. Methods: Immunohistochemistry was used to detect expression of Col1, FN and TGF-β1 in skin and scar tissue. Fibroblasts were stimulated with TGF-β1 to mimic scar formation. LB or MAPK inhibitors were used to study the pathways involved in the process. Western blotting was used to evaluate the expression of p-JNK, p-ERK, p-p38, Col1 and FN. The contractile capacity of fibroblasts was evaluated using a gel contraction assay. Tissues were cultured ex vivo with LB to further investigate the participation of ERK and JNK in the LB-mediated inhibition of scar formation. Results: FN and Col1 were up regulated in hypertrophic scars. LB down regulated p-ERK and p-JNK in TGF-β1-stimulated fibroblasts, while levels of phosphorylated p38 did not change. The down regulation of p-ERK and p-JNK was associated with a reduction of Col1 and FN. Similarly, inhibition of ERK and JNK down regulated the expression of Col1 and FN in TGF-β1-stimulated fibroblasts. LB down regulated protein levels of p-ERK and p-JNK in cultured hypertrophic scar tissue ex vivo. Conclusions: This study suggests that LB can inhibit scar formation through the ERK/JNK pathway.http://www.karger.com/Article/FullText/430385Extracellular MatrixLoureirin BHypertrophic ScarFibroblastsMAPK Pathway |
| spellingShingle | Ting He Xiaozhi Bai Longlong Yang Lei Fan Yan Li Linlin Su Jianxin Gao Shichao Han Dahai Hu Loureirin B Inhibits Hypertrophic Scar Formation via Inhibition of the TGF-β1-ERK/JNK Pathway Cellular Physiology and Biochemistry Extracellular Matrix Loureirin B Hypertrophic Scar Fibroblasts MAPK Pathway |
| title | Loureirin B Inhibits Hypertrophic Scar Formation via Inhibition of the TGF-β1-ERK/JNK Pathway |
| title_full | Loureirin B Inhibits Hypertrophic Scar Formation via Inhibition of the TGF-β1-ERK/JNK Pathway |
| title_fullStr | Loureirin B Inhibits Hypertrophic Scar Formation via Inhibition of the TGF-β1-ERK/JNK Pathway |
| title_full_unstemmed | Loureirin B Inhibits Hypertrophic Scar Formation via Inhibition of the TGF-β1-ERK/JNK Pathway |
| title_short | Loureirin B Inhibits Hypertrophic Scar Formation via Inhibition of the TGF-β1-ERK/JNK Pathway |
| title_sort | loureirin b inhibits hypertrophic scar formation via inhibition of the tgf β1 erk jnk pathway |
| topic | Extracellular Matrix Loureirin B Hypertrophic Scar Fibroblasts MAPK Pathway |
| url | http://www.karger.com/Article/FullText/430385 |
| work_keys_str_mv | AT tinghe loureirinbinhibitshypertrophicscarformationviainhibitionofthetgfb1erkjnkpathway AT xiaozhibai loureirinbinhibitshypertrophicscarformationviainhibitionofthetgfb1erkjnkpathway AT longlongyang loureirinbinhibitshypertrophicscarformationviainhibitionofthetgfb1erkjnkpathway AT leifan loureirinbinhibitshypertrophicscarformationviainhibitionofthetgfb1erkjnkpathway AT yanli loureirinbinhibitshypertrophicscarformationviainhibitionofthetgfb1erkjnkpathway AT linlinsu loureirinbinhibitshypertrophicscarformationviainhibitionofthetgfb1erkjnkpathway AT jianxingao loureirinbinhibitshypertrophicscarformationviainhibitionofthetgfb1erkjnkpathway AT shichaohan loureirinbinhibitshypertrophicscarformationviainhibitionofthetgfb1erkjnkpathway AT dahaihu loureirinbinhibitshypertrophicscarformationviainhibitionofthetgfb1erkjnkpathway |