MicroRNA-362-3p attenuates motor deficit following spinal cord injury via targeting paired box gene 2
Spinal cord injury is a disabling disorder, leading to neurological impairments. Although some microRNAs have been reported to be associated with spinal cord injury, the function of microRNA-362-3p, as one of downregulated miRNAs after spinal cord injury, is still unclear. In current study, spinal c...
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| Format: | Article |
| Language: | English |
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IMR Press
2019-03-01
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| Series: | Journal of Integrative Neuroscience |
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| Online Access: | https://jin.imrpress.com/fileup/1757-448X/PDF/1555335530494-1076740446.pdf |
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| author | Yaguang Hu, Qian Liu, Min Zhang, Yousheng Yan, Hongmei Yu, Li Ge |
| author_facet | Yaguang Hu, Qian Liu, Min Zhang, Yousheng Yan, Hongmei Yu, Li Ge |
| author_sort | Yaguang Hu, Qian Liu, Min Zhang, Yousheng Yan, Hongmei Yu, Li Ge |
| collection | DOAJ |
| description | Spinal cord injury is a disabling disorder, leading to neurological impairments. Although some microRNAs have been reported to be associated with spinal cord injury, the function of microRNA-362-3p, as one of downregulated miRNAs after spinal cord injury, is still unclear. In current study, spinal cord injury models were established. Then, we performed microRNA-362-3p overexpression in spinal cord injury rats, which expressed the low microRNA-362-3p. Results from behavioral testing, hematoxylin and eosin staining and Nissl staining revealed that microRNA-362-3p over expresssion improved the functional resoration in spinal cord injury rats. Furthermore, it caused the decrease of neuronal apoptosis and inhibition of the neuronal inflammation in these rats. Besides, Paired box gene 2 was verified as a target gene of microRNA-362-3p using luciferase assay, which predicted via bioinformatics technology. Moreover, microRNA-362-3p alleviated the neuralgia and reduced the activation of ERK and p38 through inhibition of Paired box gene 2. In conclusion, the findings demonstrated that microRNA-362-3p attenuated neuropathic pain following spinal cord injury through targeting Paired box gene 2. It provides us the new biomarker to diagnose and monitor spinal cord injury. |
| first_indexed | 2024-04-13T20:47:09Z |
| format | Article |
| id | doaj.art-578f163658e84dc2b670a4f51d605e11 |
| institution | Directory Open Access Journal |
| issn | 1757-448X |
| language | English |
| last_indexed | 2024-04-13T20:47:09Z |
| publishDate | 2019-03-01 |
| publisher | IMR Press |
| record_format | Article |
| series | Journal of Integrative Neuroscience |
| spelling | doaj.art-578f163658e84dc2b670a4f51d605e112022-12-22T02:30:38ZengIMR PressJournal of Integrative Neuroscience1757-448X2019-03-01181576410.31083/j.jin.2019.01.12MicroRNA-362-3p attenuates motor deficit following spinal cord injury via targeting paired box gene 2Yaguang Hu, Qian Liu, Min Zhang, Yousheng Yan, Hongmei Yu, Li Ge01 Department of Pharmacy, Gansu Provincial Maternity and Child-care Hospital; Lanzhou City, Gansu Province, 730050, China;2 Department of Cerebrovascular Disease Center, Gansu Provincial Hospital; Lanzhou City, Gansu Province, 730000, China;3 Department of Pathology, Gansu Provincial Hospital; Lanzhou City, Gansu Province, 730000, China;4 National Research Institute for Health and Family Planning; National Centre for Human Genetic Resources; Beijing, 100089, China;5 Department of Neurological Rehabilitation, Gansu Provincial Hospital; Lanzhou City, Gansu Province, 730000, ChinaSpinal cord injury is a disabling disorder, leading to neurological impairments. Although some microRNAs have been reported to be associated with spinal cord injury, the function of microRNA-362-3p, as one of downregulated miRNAs after spinal cord injury, is still unclear. In current study, spinal cord injury models were established. Then, we performed microRNA-362-3p overexpression in spinal cord injury rats, which expressed the low microRNA-362-3p. Results from behavioral testing, hematoxylin and eosin staining and Nissl staining revealed that microRNA-362-3p over expresssion improved the functional resoration in spinal cord injury rats. Furthermore, it caused the decrease of neuronal apoptosis and inhibition of the neuronal inflammation in these rats. Besides, Paired box gene 2 was verified as a target gene of microRNA-362-3p using luciferase assay, which predicted via bioinformatics technology. Moreover, microRNA-362-3p alleviated the neuralgia and reduced the activation of ERK and p38 through inhibition of Paired box gene 2. In conclusion, the findings demonstrated that microRNA-362-3p attenuated neuropathic pain following spinal cord injury through targeting Paired box gene 2. It provides us the new biomarker to diagnose and monitor spinal cord injury.https://jin.imrpress.com/fileup/1757-448X/PDF/1555335530494-1076740446.pdf|spinal cord injury|mir-362-3p|pax2|neuralgia |
| spellingShingle | Yaguang Hu, Qian Liu, Min Zhang, Yousheng Yan, Hongmei Yu, Li Ge MicroRNA-362-3p attenuates motor deficit following spinal cord injury via targeting paired box gene 2 Journal of Integrative Neuroscience |spinal cord injury|mir-362-3p|pax2|neuralgia |
| title | MicroRNA-362-3p attenuates motor deficit following spinal cord injury via targeting paired box gene 2 |
| title_full | MicroRNA-362-3p attenuates motor deficit following spinal cord injury via targeting paired box gene 2 |
| title_fullStr | MicroRNA-362-3p attenuates motor deficit following spinal cord injury via targeting paired box gene 2 |
| title_full_unstemmed | MicroRNA-362-3p attenuates motor deficit following spinal cord injury via targeting paired box gene 2 |
| title_short | MicroRNA-362-3p attenuates motor deficit following spinal cord injury via targeting paired box gene 2 |
| title_sort | microrna 362 3p attenuates motor deficit following spinal cord injury via targeting paired box gene 2 |
| topic | |spinal cord injury|mir-362-3p|pax2|neuralgia |
| url | https://jin.imrpress.com/fileup/1757-448X/PDF/1555335530494-1076740446.pdf |
| work_keys_str_mv | AT yaguanghuqianliuminzhangyoushengyanhongmeiyulige microrna3623pattenuatesmotordeficitfollowingspinalcordinjuryviatargetingpairedboxgene2 |