The interaction of macrophages and CD8 T cells in bronchoalveolar lavage fluid is associated with latent tuberculosis infection
ABSTRACTMycobacterium tuberculosis (Mtb) infection, including active tuberculosis (TB) and latent Mtb infection (LTBI), leads to diverse outcomes owing to different host immune responses. However, the immune mechanisms that govern the progression from LTBI to TB remain poorly defined in humans. Here...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2023.2239940 |
| _version_ | 1797257051165949952 |
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| author | Qianting Yang Furong Qi Taosheng Ye Jinpei Li Gang Xu Xiaomeng He Guofang Deng Peize Zhang Mingfeng Liao Kun Qiao Zheng Zhang |
| author_facet | Qianting Yang Furong Qi Taosheng Ye Jinpei Li Gang Xu Xiaomeng He Guofang Deng Peize Zhang Mingfeng Liao Kun Qiao Zheng Zhang |
| author_sort | Qianting Yang |
| collection | DOAJ |
| description | ABSTRACTMycobacterium tuberculosis (Mtb) infection, including active tuberculosis (TB) and latent Mtb infection (LTBI), leads to diverse outcomes owing to different host immune responses. However, the immune mechanisms that govern the progression from LTBI to TB remain poorly defined in humans. Here, we profiled the lung immune cell populations within the bronchoalveolar lavage fluid (BALF) from patients with LTBI or TB using single-cell RNA sequencing (scRNA-seq). We found that Mtb infection substantially changed the immune cell compartments in the BALF, especially for the three subsets of macrophages, monocyte macrophage (MM)-CCL23, MM-FCN1, and MM-SPP1, which were found to be associated with the disease status of TB infection. Notably, MM-CCL23 cells derived from monocytes after stimulation with Mtb were characterized by high levels of chemokine (CCL23 and CXCL5) production and might serve as a marker for Mtb infection. The MM-CCL23 population mainly recruited CD8-CCR6 T cells through CCL20/CCR6, which was a prominent feature associated with protection immunity in LTBI. This study improves our understanding of the lung immune landscape during Mtb infection, which may inform future vaccine design for protective immunity. |
| first_indexed | 2024-03-08T11:51:20Z |
| format | Article |
| id | doaj.art-57952a54f8094b1bba02badbd17ff2ff |
| institution | Directory Open Access Journal |
| issn | 2222-1751 |
| language | English |
| last_indexed | 2024-04-24T22:31:29Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj.art-57952a54f8094b1bba02badbd17ff2ff2024-03-19T19:34:17ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512023-12-0112210.1080/22221751.2023.2239940The interaction of macrophages and CD8 T cells in bronchoalveolar lavage fluid is associated with latent tuberculosis infectionQianting Yang0Furong Qi1Taosheng Ye2Jinpei Li3Gang Xu4Xiaomeng He5Guofang Deng6Peize Zhang7Mingfeng Liao8Kun Qiao9Zheng Zhang10Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital; The Second Affiliated Hospital, School of Medicine, Southern University of Science and Technology, Shenzhen, People’s Republic of ChinaInstitute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital; The Second Affiliated Hospital, School of Medicine, Southern University of Science and Technology, Shenzhen, People’s Republic of ChinaShenzhen Clinical Research Center for Tuberculosis, Shenzhen, People’s Republic of ChinaShenzhen Clinical Research Center for Tuberculosis, Shenzhen, People’s Republic of ChinaInstitute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital; The Second Affiliated Hospital, School of Medicine, Southern University of Science and Technology, Shenzhen, People’s Republic of ChinaInstitute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital; The Second Affiliated Hospital, School of Medicine, Southern University of Science and Technology, Shenzhen, People’s Republic of ChinaShenzhen Clinical Research Center for Tuberculosis, Shenzhen, People’s Republic of ChinaShenzhen Clinical Research Center for Tuberculosis, Shenzhen, People’s Republic of ChinaInstitute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital; The Second Affiliated Hospital, School of Medicine, Southern University of Science and Technology, Shenzhen, People’s Republic of ChinaShenzhen Clinical Research Center for Tuberculosis, Shenzhen, People’s Republic of ChinaInstitute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital; The Second Affiliated Hospital, School of Medicine, Southern University of Science and Technology, Shenzhen, People’s Republic of ChinaABSTRACTMycobacterium tuberculosis (Mtb) infection, including active tuberculosis (TB) and latent Mtb infection (LTBI), leads to diverse outcomes owing to different host immune responses. However, the immune mechanisms that govern the progression from LTBI to TB remain poorly defined in humans. Here, we profiled the lung immune cell populations within the bronchoalveolar lavage fluid (BALF) from patients with LTBI or TB using single-cell RNA sequencing (scRNA-seq). We found that Mtb infection substantially changed the immune cell compartments in the BALF, especially for the three subsets of macrophages, monocyte macrophage (MM)-CCL23, MM-FCN1, and MM-SPP1, which were found to be associated with the disease status of TB infection. Notably, MM-CCL23 cells derived from monocytes after stimulation with Mtb were characterized by high levels of chemokine (CCL23 and CXCL5) production and might serve as a marker for Mtb infection. The MM-CCL23 population mainly recruited CD8-CCR6 T cells through CCL20/CCR6, which was a prominent feature associated with protection immunity in LTBI. This study improves our understanding of the lung immune landscape during Mtb infection, which may inform future vaccine design for protective immunity.https://www.tandfonline.com/doi/10.1080/22221751.2023.2239940TuberculosisSingle-cell RNA sequencingMacrophageCD8T cellImmunoregulation |
| spellingShingle | Qianting Yang Furong Qi Taosheng Ye Jinpei Li Gang Xu Xiaomeng He Guofang Deng Peize Zhang Mingfeng Liao Kun Qiao Zheng Zhang The interaction of macrophages and CD8 T cells in bronchoalveolar lavage fluid is associated with latent tuberculosis infection Emerging Microbes and Infections Tuberculosis Single-cell RNA sequencing Macrophage CD8T cell Immunoregulation |
| title | The interaction of macrophages and CD8 T cells in bronchoalveolar lavage fluid is associated with latent tuberculosis infection |
| title_full | The interaction of macrophages and CD8 T cells in bronchoalveolar lavage fluid is associated with latent tuberculosis infection |
| title_fullStr | The interaction of macrophages and CD8 T cells in bronchoalveolar lavage fluid is associated with latent tuberculosis infection |
| title_full_unstemmed | The interaction of macrophages and CD8 T cells in bronchoalveolar lavage fluid is associated with latent tuberculosis infection |
| title_short | The interaction of macrophages and CD8 T cells in bronchoalveolar lavage fluid is associated with latent tuberculosis infection |
| title_sort | interaction of macrophages and cd8 t cells in bronchoalveolar lavage fluid is associated with latent tuberculosis infection |
| topic | Tuberculosis Single-cell RNA sequencing Macrophage CD8T cell Immunoregulation |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2023.2239940 |
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