Transcriptome Analysis of Protocatechualdehyde against <i>Listeria monocytogenes</i> and Its Effect on Chicken Quality Characteristics
The development of natural antimicrobial agents offers new strategies for food preservation due to the health hazards associated with the spoilage of meat products caused by microbial contamination. In this paper, the inhibitory mechanism of protocatechualdehyde (PCA) on <i>Listeria monocytoge...
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MDPI AG
2023-07-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/13/2625 |
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| author | Sichen Liao Lu Tian Qi Qi Lemei Hu Minmin Wang Chang Gao Haoyue Cui Zhongchao Gai Guoli Gong |
| author_facet | Sichen Liao Lu Tian Qi Qi Lemei Hu Minmin Wang Chang Gao Haoyue Cui Zhongchao Gai Guoli Gong |
| author_sort | Sichen Liao |
| collection | DOAJ |
| description | The development of natural antimicrobial agents offers new strategies for food preservation due to the health hazards associated with the spoilage of meat products caused by microbial contamination. In this paper, the inhibitory mechanism of protocatechualdehyde (PCA) on <i>Listeria monocytogenes</i> was described, and its effect on the preservation of cooked chicken breast was evaluated. The results showed that the minimal inhibitory concentration (MIC) of PCA on <i>L. monocytogenes</i> was 0.625 mg/mL. Secondly, PCA destroyed the integrity of the <i>L. monocytogenes</i> cell membrane, which was manifested as a decrease in membrane hyperpolarization, intracellular ATP level, and intracellular pH value. Field emission gun scanning electron microscopy (FEG-SEM) observed a cell membrane rupture. Transcriptome analysis showed that PCA may inhibit cell growth by affecting amino acid, nucleotide metabolism, energy metabolism, and the cell membrane of <i>L. monocytogenes</i>. Additionally, it was discovered that PCA enhanced the color and texture of cooked chicken breast meat while decreasing the level of thiobarbituric acid active substance (TBARS). In conclusion, PCA as a natural antibacterial agent has a certain reference value in extending the shelf life of cooked chicken breast. |
| first_indexed | 2024-03-11T01:40:59Z |
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| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-11T01:40:59Z |
| publishDate | 2023-07-01 |
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| series | Foods |
| spelling | doaj.art-579542cda2d44631a230bfdce58c65ee2023-11-18T16:35:15ZengMDPI AGFoods2304-81582023-07-011213262510.3390/foods12132625Transcriptome Analysis of Protocatechualdehyde against <i>Listeria monocytogenes</i> and Its Effect on Chicken Quality CharacteristicsSichen Liao0Lu Tian1Qi Qi2Lemei Hu3Minmin Wang4Chang Gao5Haoyue Cui6Zhongchao Gai7Guoli Gong8School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi’an 710021, ChinaSchool of Food Science and Engineering, Shaanxi University of Science and Technology, Xi’an 710021, ChinaSchool of Food Science and Engineering, Shaanxi University of Science and Technology, Xi’an 710021, ChinaSchool of Food Science and Engineering, Shaanxi University of Science and Technology, Xi’an 710021, ChinaSchool of Food Science and Engineering, Shaanxi University of Science and Technology, Xi’an 710021, ChinaSchool of Food Science and Engineering, Shaanxi University of Science and Technology, Xi’an 710021, ChinaSchool of Food Science and Engineering, Shaanxi University of Science and Technology, Xi’an 710021, ChinaSchool of Food Science and Engineering, Shaanxi University of Science and Technology, Xi’an 710021, ChinaSchool of Food Science and Engineering, Shaanxi University of Science and Technology, Xi’an 710021, ChinaThe development of natural antimicrobial agents offers new strategies for food preservation due to the health hazards associated with the spoilage of meat products caused by microbial contamination. In this paper, the inhibitory mechanism of protocatechualdehyde (PCA) on <i>Listeria monocytogenes</i> was described, and its effect on the preservation of cooked chicken breast was evaluated. The results showed that the minimal inhibitory concentration (MIC) of PCA on <i>L. monocytogenes</i> was 0.625 mg/mL. Secondly, PCA destroyed the integrity of the <i>L. monocytogenes</i> cell membrane, which was manifested as a decrease in membrane hyperpolarization, intracellular ATP level, and intracellular pH value. Field emission gun scanning electron microscopy (FEG-SEM) observed a cell membrane rupture. Transcriptome analysis showed that PCA may inhibit cell growth by affecting amino acid, nucleotide metabolism, energy metabolism, and the cell membrane of <i>L. monocytogenes</i>. Additionally, it was discovered that PCA enhanced the color and texture of cooked chicken breast meat while decreasing the level of thiobarbituric acid active substance (TBARS). In conclusion, PCA as a natural antibacterial agent has a certain reference value in extending the shelf life of cooked chicken breast.https://www.mdpi.com/2304-8158/12/13/2625protocatechualdehyde<i>Listeria monocytogenes</i>antibacterial activityaction mechanismtranscriptomecooked chicken breast meat quality |
| spellingShingle | Sichen Liao Lu Tian Qi Qi Lemei Hu Minmin Wang Chang Gao Haoyue Cui Zhongchao Gai Guoli Gong Transcriptome Analysis of Protocatechualdehyde against <i>Listeria monocytogenes</i> and Its Effect on Chicken Quality Characteristics Foods protocatechualdehyde <i>Listeria monocytogenes</i> antibacterial activity action mechanism transcriptome cooked chicken breast meat quality |
| title | Transcriptome Analysis of Protocatechualdehyde against <i>Listeria monocytogenes</i> and Its Effect on Chicken Quality Characteristics |
| title_full | Transcriptome Analysis of Protocatechualdehyde against <i>Listeria monocytogenes</i> and Its Effect on Chicken Quality Characteristics |
| title_fullStr | Transcriptome Analysis of Protocatechualdehyde against <i>Listeria monocytogenes</i> and Its Effect on Chicken Quality Characteristics |
| title_full_unstemmed | Transcriptome Analysis of Protocatechualdehyde against <i>Listeria monocytogenes</i> and Its Effect on Chicken Quality Characteristics |
| title_short | Transcriptome Analysis of Protocatechualdehyde against <i>Listeria monocytogenes</i> and Its Effect on Chicken Quality Characteristics |
| title_sort | transcriptome analysis of protocatechualdehyde against i listeria monocytogenes i and its effect on chicken quality characteristics |
| topic | protocatechualdehyde <i>Listeria monocytogenes</i> antibacterial activity action mechanism transcriptome cooked chicken breast meat quality |
| url | https://www.mdpi.com/2304-8158/12/13/2625 |
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