Immunogenic Properties and Antigenic Similarity of Virus-like Particles Derived from Human Polyomaviruses
Polyomaviruses (PyVs) are highly prevalent in humans and animals. PyVs cause mild illness, however, they can also elicit severe diseases. Some PyVs are potentially zoonotic, such as simian virus 40 (SV40). However, data are still lacking about their biology, infectivity, and host interaction with di...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/5/4907 |
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| author | Asta Lučiūnaitė Indrė Dalgėdienė Emilija Vasiliūnaitė Milda Norkienė Indrė Kučinskaitė-Kodzė Aurelija Žvirblienė Alma Gedvilaitė |
| author_facet | Asta Lučiūnaitė Indrė Dalgėdienė Emilija Vasiliūnaitė Milda Norkienė Indrė Kučinskaitė-Kodzė Aurelija Žvirblienė Alma Gedvilaitė |
| author_sort | Asta Lučiūnaitė |
| collection | DOAJ |
| description | Polyomaviruses (PyVs) are highly prevalent in humans and animals. PyVs cause mild illness, however, they can also elicit severe diseases. Some PyVs are potentially zoonotic, such as simian virus 40 (SV40). However, data are still lacking about their biology, infectivity, and host interaction with different PyVs. We investigated the immunogenic properties of virus-like particles (VLPs) derived from viral protein 1 (VP1) of human PyVs. We immunised mice with recombinant HPyV VP1 VLPs mimicking the structure of viruses and compared their immunogenicity and cross-reactivity of antisera using a broad spectrum of VP1 VLPs derived from the PyVs of humans and animals. We demonstrated a strong immunogenicity of studied VLPs and a high degree of antigenic similarity between VP1 VLPs of different PyVs. PyV-specific monoclonal antibodies were generated and applied for investigation of VLPs phagocytosis. This study demonstrated that HPyV VLPs are highly immunogenic and interact with phagocytes. Data on the cross-reactivity of VP1 VLP-specific antisera revealed antigenic similarities among VP1 VLPs of particular human and animal PyVs and suggested possible cross-immunity. As the VP1 capsid protein is the major viral antigen involved in virus-host interaction, an approach based on the use of recombinant VLPs is relevant for studying PyV biology regarding PyV interaction with the host immune system. |
| first_indexed | 2024-03-11T07:21:29Z |
| format | Article |
| id | doaj.art-57965fac3d034bd4885fe441174342c9 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T07:21:29Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-57965fac3d034bd4885fe441174342c92023-11-17T07:55:05ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-03-01245490710.3390/ijms24054907Immunogenic Properties and Antigenic Similarity of Virus-like Particles Derived from Human PolyomavirusesAsta Lučiūnaitė0Indrė Dalgėdienė1Emilija Vasiliūnaitė2Milda Norkienė3Indrė Kučinskaitė-Kodzė4Aurelija Žvirblienė5Alma Gedvilaitė6Institute of Biotechnology, Life Sciences Center, Vilnius University, 7 Saulėtekio Ave, LT-10257 Vilnius, LithuaniaInstitute of Biotechnology, Life Sciences Center, Vilnius University, 7 Saulėtekio Ave, LT-10257 Vilnius, LithuaniaInstitute of Biotechnology, Life Sciences Center, Vilnius University, 7 Saulėtekio Ave, LT-10257 Vilnius, LithuaniaInstitute of Biotechnology, Life Sciences Center, Vilnius University, 7 Saulėtekio Ave, LT-10257 Vilnius, LithuaniaInstitute of Biotechnology, Life Sciences Center, Vilnius University, 7 Saulėtekio Ave, LT-10257 Vilnius, LithuaniaInstitute of Biotechnology, Life Sciences Center, Vilnius University, 7 Saulėtekio Ave, LT-10257 Vilnius, LithuaniaInstitute of Biotechnology, Life Sciences Center, Vilnius University, 7 Saulėtekio Ave, LT-10257 Vilnius, LithuaniaPolyomaviruses (PyVs) are highly prevalent in humans and animals. PyVs cause mild illness, however, they can also elicit severe diseases. Some PyVs are potentially zoonotic, such as simian virus 40 (SV40). However, data are still lacking about their biology, infectivity, and host interaction with different PyVs. We investigated the immunogenic properties of virus-like particles (VLPs) derived from viral protein 1 (VP1) of human PyVs. We immunised mice with recombinant HPyV VP1 VLPs mimicking the structure of viruses and compared their immunogenicity and cross-reactivity of antisera using a broad spectrum of VP1 VLPs derived from the PyVs of humans and animals. We demonstrated a strong immunogenicity of studied VLPs and a high degree of antigenic similarity between VP1 VLPs of different PyVs. PyV-specific monoclonal antibodies were generated and applied for investigation of VLPs phagocytosis. This study demonstrated that HPyV VLPs are highly immunogenic and interact with phagocytes. Data on the cross-reactivity of VP1 VLP-specific antisera revealed antigenic similarities among VP1 VLPs of particular human and animal PyVs and suggested possible cross-immunity. As the VP1 capsid protein is the major viral antigen involved in virus-host interaction, an approach based on the use of recombinant VLPs is relevant for studying PyV biology regarding PyV interaction with the host immune system.https://www.mdpi.com/1422-0067/24/5/4907polyomavirusviral antigensantibodiesimmune responsemacrophages |
| spellingShingle | Asta Lučiūnaitė Indrė Dalgėdienė Emilija Vasiliūnaitė Milda Norkienė Indrė Kučinskaitė-Kodzė Aurelija Žvirblienė Alma Gedvilaitė Immunogenic Properties and Antigenic Similarity of Virus-like Particles Derived from Human Polyomaviruses International Journal of Molecular Sciences polyomavirus viral antigens antibodies immune response macrophages |
| title | Immunogenic Properties and Antigenic Similarity of Virus-like Particles Derived from Human Polyomaviruses |
| title_full | Immunogenic Properties and Antigenic Similarity of Virus-like Particles Derived from Human Polyomaviruses |
| title_fullStr | Immunogenic Properties and Antigenic Similarity of Virus-like Particles Derived from Human Polyomaviruses |
| title_full_unstemmed | Immunogenic Properties and Antigenic Similarity of Virus-like Particles Derived from Human Polyomaviruses |
| title_short | Immunogenic Properties and Antigenic Similarity of Virus-like Particles Derived from Human Polyomaviruses |
| title_sort | immunogenic properties and antigenic similarity of virus like particles derived from human polyomaviruses |
| topic | polyomavirus viral antigens antibodies immune response macrophages |
| url | https://www.mdpi.com/1422-0067/24/5/4907 |
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