Pink-Beam Serial Synchrotron Crystallography at Pohang Light Source II
Serial crystallography (SX) enables the determination of room-temperature structures with minimal radiation damage. The photon flux of the pink beam of 1.2% bandwidth (BW) is one order higher than that of the monochromatic beam from a silicon crystal monochromator, and the energy resolution of 1.2%...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Crystals |
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| Online Access: | https://www.mdpi.com/2073-4352/12/11/1637 |
| _version_ | 1797465571856482304 |
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| author | Yongsam Kim Ki Hyun Nam |
| author_facet | Yongsam Kim Ki Hyun Nam |
| author_sort | Yongsam Kim |
| collection | DOAJ |
| description | Serial crystallography (SX) enables the determination of room-temperature structures with minimal radiation damage. The photon flux of the pink beam of 1.2% bandwidth (BW) is one order higher than that of the monochromatic beam from a silicon crystal monochromator, and the energy resolution of 1.2% BW is enough to solve the structure; therefore, it is useful to use the pink beam for time-resolved serial synchrotron crystallography (SSX). Here, we demonstrate a pink-beam serial synchrotron crystallographic study at the 1C beamline at the Pohang Light Source II. Lysozyme crystals embedded in a beef tallow injection matrix were delivered through a syringe into the X-ray interaction point. Pink-beam SSX was performed with different X-ray exposure positions to the injection stream (center and edge) and X-ray exposure times (50 and 100 ms). All lysozyme crystal structures were successfully determined at a high resolution of 1.7 Å. Background analysis showed that X-ray diffraction data exposed to the edge of the injection stream could improve the signal-to-noise ratio. All the diffraction data and room-temperature lysozyme structures were comprehensively compared. The data collection strategy and analysis will be helpful in further pink-beam SSX experiments and their applications. |
| first_indexed | 2024-03-09T18:24:23Z |
| format | Article |
| id | doaj.art-579a58d5f729410bbca3c6732b1a8a4c |
| institution | Directory Open Access Journal |
| issn | 2073-4352 |
| language | English |
| last_indexed | 2024-03-09T18:24:23Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Crystals |
| spelling | doaj.art-579a58d5f729410bbca3c6732b1a8a4c2023-11-24T08:02:16ZengMDPI AGCrystals2073-43522022-11-011211163710.3390/cryst12111637Pink-Beam Serial Synchrotron Crystallography at Pohang Light Source IIYongsam Kim0Ki Hyun Nam1Pohang Accelerator Laboratory, Pohang University of Science and Technology, Pohang 37673, KoreaDepartment of Life Science, Pohang University of Science and Technology, Pohang 37673, KoreaSerial crystallography (SX) enables the determination of room-temperature structures with minimal radiation damage. The photon flux of the pink beam of 1.2% bandwidth (BW) is one order higher than that of the monochromatic beam from a silicon crystal monochromator, and the energy resolution of 1.2% BW is enough to solve the structure; therefore, it is useful to use the pink beam for time-resolved serial synchrotron crystallography (SSX). Here, we demonstrate a pink-beam serial synchrotron crystallographic study at the 1C beamline at the Pohang Light Source II. Lysozyme crystals embedded in a beef tallow injection matrix were delivered through a syringe into the X-ray interaction point. Pink-beam SSX was performed with different X-ray exposure positions to the injection stream (center and edge) and X-ray exposure times (50 and 100 ms). All lysozyme crystal structures were successfully determined at a high resolution of 1.7 Å. Background analysis showed that X-ray diffraction data exposed to the edge of the injection stream could improve the signal-to-noise ratio. All the diffraction data and room-temperature lysozyme structures were comprehensively compared. The data collection strategy and analysis will be helpful in further pink-beam SSX experiments and their applications.https://www.mdpi.com/2073-4352/12/11/1637serial crystallographypink-beamsynchrotronroom-temperaturecrystal structure |
| spellingShingle | Yongsam Kim Ki Hyun Nam Pink-Beam Serial Synchrotron Crystallography at Pohang Light Source II Crystals serial crystallography pink-beam synchrotron room-temperature crystal structure |
| title | Pink-Beam Serial Synchrotron Crystallography at Pohang Light Source II |
| title_full | Pink-Beam Serial Synchrotron Crystallography at Pohang Light Source II |
| title_fullStr | Pink-Beam Serial Synchrotron Crystallography at Pohang Light Source II |
| title_full_unstemmed | Pink-Beam Serial Synchrotron Crystallography at Pohang Light Source II |
| title_short | Pink-Beam Serial Synchrotron Crystallography at Pohang Light Source II |
| title_sort | pink beam serial synchrotron crystallography at pohang light source ii |
| topic | serial crystallography pink-beam synchrotron room-temperature crystal structure |
| url | https://www.mdpi.com/2073-4352/12/11/1637 |
| work_keys_str_mv | AT yongsamkim pinkbeamserialsynchrotroncrystallographyatpohanglightsourceii AT kihyunnam pinkbeamserialsynchrotroncrystallographyatpohanglightsourceii |