Isolation and Identification of Hydrocarbon-Degrading Bacteria from Polychaete Marphysa moribidii
Marine contamination caused by anthropogenic activities has side effects and causes severe contamination to the environment. Polychaetes are benthic organisms that live in the sediment and can be a good indicator of sediment contamination by organic compounds. In this study, bacterial strains were i...
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| Format: | Article |
| Language: | English |
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Kementerian Kelautan dan Perikanan
2021-12-01
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| Series: | Squalen |
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| Online Access: | https://www.bbp4b.litbang.kkp.go.id/squalen-bulletin/index.php/squalen/article/view/567 |
| _version_ | 1819009035784945664 |
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| author | Fazilah Arifin Rozilawati Mohamed Razali Vigneswari Sevakumaran |
| author_facet | Fazilah Arifin Rozilawati Mohamed Razali Vigneswari Sevakumaran |
| author_sort | Fazilah Arifin |
| collection | DOAJ |
| description | Marine contamination caused by anthropogenic activities has side effects and causes severe contamination to the environment. Polychaetes are benthic organisms that live in the sediment and can be a good indicator of sediment contamination by organic compounds. In this study, bacterial strains were isolated and identified from the gut of polychaete worm Marphysa moribidii and the potential of the bacteria was evaluated to degrade hydrocarbon compounds. The isolated bacteria were primary and secondary screened on Minimal Salt Media (MSM) agar supplemented with 1% v/v of diesel oil. Diesel degradation analysis was performed by inoculating potential bacterium into MSM broth with 1% v/v diesel oil and incubated at 37 oC for 20 days. Diesel degradation percentage was analyzed using the gravimetric method, while the bacteria cell densities were measured using the standard plate count method. Then, the selected isolates were identified based on their morphological characteristics and 16S rDNA sequences. As a result, two bacteria isolates coded as Isolate 6 and Isolate 8 were able to degrade diesel oil up to 52.29% and 39.24% after 20 days of incubation. The 16S rDNA sequence analysis revealed that it was identified as Bacillus sp. strain UMTFA1 (RB) and Staphylococcus kloosii strain UMTFA2 (RS). Our result showed that these strains have the potential in oil-degrading processes, which will provide new insight into bioremediation process and decrease environmental pollution in soil and water contaminated with hydrocarbons. |
| first_indexed | 2024-12-21T00:49:58Z |
| format | Article |
| id | doaj.art-579b0b6fc9be4f429ec201bb1fd5b5ed |
| institution | Directory Open Access Journal |
| issn | 2089-5690 2406-9272 |
| language | English |
| last_indexed | 2024-12-21T00:49:58Z |
| publishDate | 2021-12-01 |
| publisher | Kementerian Kelautan dan Perikanan |
| record_format | Article |
| series | Squalen |
| spelling | doaj.art-579b0b6fc9be4f429ec201bb1fd5b5ed2022-12-21T19:21:26ZengKementerian Kelautan dan PerikananSqualen2089-56902406-92722021-12-0116313013810.15578/squalen.567310Isolation and Identification of Hydrocarbon-Degrading Bacteria from Polychaete Marphysa moribidiiFazilah Arifin0Rozilawati Mohamed Razali1Vigneswari Sevakumaran2Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia Biological Security and Sustainability Research Group, Universiti Malaysia Terengganu, 21300 Kuala Nerus, Terengganu, MalaysiaFaculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, MalaysiaFaculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia Biological Security and Sustainability Research Group, Universiti Malaysia Terengganu, 21300 Kuala Nerus, Terengganu, MalaysiaMarine contamination caused by anthropogenic activities has side effects and causes severe contamination to the environment. Polychaetes are benthic organisms that live in the sediment and can be a good indicator of sediment contamination by organic compounds. In this study, bacterial strains were isolated and identified from the gut of polychaete worm Marphysa moribidii and the potential of the bacteria was evaluated to degrade hydrocarbon compounds. The isolated bacteria were primary and secondary screened on Minimal Salt Media (MSM) agar supplemented with 1% v/v of diesel oil. Diesel degradation analysis was performed by inoculating potential bacterium into MSM broth with 1% v/v diesel oil and incubated at 37 oC for 20 days. Diesel degradation percentage was analyzed using the gravimetric method, while the bacteria cell densities were measured using the standard plate count method. Then, the selected isolates were identified based on their morphological characteristics and 16S rDNA sequences. As a result, two bacteria isolates coded as Isolate 6 and Isolate 8 were able to degrade diesel oil up to 52.29% and 39.24% after 20 days of incubation. The 16S rDNA sequence analysis revealed that it was identified as Bacillus sp. strain UMTFA1 (RB) and Staphylococcus kloosii strain UMTFA2 (RS). Our result showed that these strains have the potential in oil-degrading processes, which will provide new insight into bioremediation process and decrease environmental pollution in soil and water contaminated with hydrocarbons.https://www.bbp4b.litbang.kkp.go.id/squalen-bulletin/index.php/squalen/article/view/567biodegradation, bacteria, diesel oil, pollutant, polychaete |
| spellingShingle | Fazilah Arifin Rozilawati Mohamed Razali Vigneswari Sevakumaran Isolation and Identification of Hydrocarbon-Degrading Bacteria from Polychaete Marphysa moribidii Squalen biodegradation, bacteria, diesel oil, pollutant, polychaete |
| title | Isolation and Identification of Hydrocarbon-Degrading Bacteria from Polychaete Marphysa moribidii |
| title_full | Isolation and Identification of Hydrocarbon-Degrading Bacteria from Polychaete Marphysa moribidii |
| title_fullStr | Isolation and Identification of Hydrocarbon-Degrading Bacteria from Polychaete Marphysa moribidii |
| title_full_unstemmed | Isolation and Identification of Hydrocarbon-Degrading Bacteria from Polychaete Marphysa moribidii |
| title_short | Isolation and Identification of Hydrocarbon-Degrading Bacteria from Polychaete Marphysa moribidii |
| title_sort | isolation and identification of hydrocarbon degrading bacteria from polychaete marphysa moribidii |
| topic | biodegradation, bacteria, diesel oil, pollutant, polychaete |
| url | https://www.bbp4b.litbang.kkp.go.id/squalen-bulletin/index.php/squalen/article/view/567 |
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