Lifeact-mEGFP reveals a dynamic apical F-actin network in tip growing plant cells.
Actin is essential for tip growth in plants. However, imaging actin in live plant cells has heretofore presented challenges. In previous studies, fluorescent probes derived from actin-binding proteins often alter growth, cause actin bundling and fail to resolve actin microfilaments.In this report we...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2009-05-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2684639?pdf=render |
| _version_ | 1819236767722635264 |
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| author | Luis Vidali Caleb M Rounds Peter K Hepler Magdalena Bezanilla |
| author_facet | Luis Vidali Caleb M Rounds Peter K Hepler Magdalena Bezanilla |
| author_sort | Luis Vidali |
| collection | DOAJ |
| description | Actin is essential for tip growth in plants. However, imaging actin in live plant cells has heretofore presented challenges. In previous studies, fluorescent probes derived from actin-binding proteins often alter growth, cause actin bundling and fail to resolve actin microfilaments.In this report we use Lifeact-mEGFP, an actin probe that does not affect the dynamics of actin, to visualize actin in the moss Physcomitrella patens and pollen tubes from Lilium formosanum and Nicotiana tobaccum. Lifeact-mEGFP robustly labels actin microfilaments, particularly in the apex, in both moss protonemata and pollen tubes. Lifeact-mEGFP also labels filamentous actin structures in other moss cell types, including cells of the gametophore.Lifeact-mEGFP, when expressed at optimal levels does not alter moss protonemal or pollen tube growth. We suggest that Lifeact-mEGFP represents an exciting new versatile probe for further studies of actin's role in tip growing plant cells. |
| first_indexed | 2024-12-23T13:09:40Z |
| format | Article |
| id | doaj.art-579dc9783e0c4f46825505f33c4a515f |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-23T13:09:40Z |
| publishDate | 2009-05-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-579dc9783e0c4f46825505f33c4a515f2022-12-21T17:45:46ZengPublic Library of Science (PLoS)PLoS ONE1932-62032009-05-0145e574410.1371/journal.pone.0005744Lifeact-mEGFP reveals a dynamic apical F-actin network in tip growing plant cells.Luis VidaliCaleb M RoundsPeter K HeplerMagdalena BezanillaActin is essential for tip growth in plants. However, imaging actin in live plant cells has heretofore presented challenges. In previous studies, fluorescent probes derived from actin-binding proteins often alter growth, cause actin bundling and fail to resolve actin microfilaments.In this report we use Lifeact-mEGFP, an actin probe that does not affect the dynamics of actin, to visualize actin in the moss Physcomitrella patens and pollen tubes from Lilium formosanum and Nicotiana tobaccum. Lifeact-mEGFP robustly labels actin microfilaments, particularly in the apex, in both moss protonemata and pollen tubes. Lifeact-mEGFP also labels filamentous actin structures in other moss cell types, including cells of the gametophore.Lifeact-mEGFP, when expressed at optimal levels does not alter moss protonemal or pollen tube growth. We suggest that Lifeact-mEGFP represents an exciting new versatile probe for further studies of actin's role in tip growing plant cells.http://europepmc.org/articles/PMC2684639?pdf=render |
| spellingShingle | Luis Vidali Caleb M Rounds Peter K Hepler Magdalena Bezanilla Lifeact-mEGFP reveals a dynamic apical F-actin network in tip growing plant cells. PLoS ONE |
| title | Lifeact-mEGFP reveals a dynamic apical F-actin network in tip growing plant cells. |
| title_full | Lifeact-mEGFP reveals a dynamic apical F-actin network in tip growing plant cells. |
| title_fullStr | Lifeact-mEGFP reveals a dynamic apical F-actin network in tip growing plant cells. |
| title_full_unstemmed | Lifeact-mEGFP reveals a dynamic apical F-actin network in tip growing plant cells. |
| title_short | Lifeact-mEGFP reveals a dynamic apical F-actin network in tip growing plant cells. |
| title_sort | lifeact megfp reveals a dynamic apical f actin network in tip growing plant cells |
| url | http://europepmc.org/articles/PMC2684639?pdf=render |
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