A novel chemotactic factor derived from the extracellular matrix protein decorin recruits mesenchymal stromal cells in vitro and in vivo.
Soft tissue is composed of cells surrounded by an extracellular matrix that is made up of a diverse array of intricately organized proteins. These distinct components work in concert to maintain homeostasis and respond to tissue damage. During tissue repair, extracellular matrix proteins and their d...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2020-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0235784 |
| _version_ | 1828922594403811328 |
|---|---|
| author | Sandi Grainne Dempsey Christopher Hamilton Miller Julia Schueler Robert W F Veale Darren J Day Barnaby C H May |
| author_facet | Sandi Grainne Dempsey Christopher Hamilton Miller Julia Schueler Robert W F Veale Darren J Day Barnaby C H May |
| author_sort | Sandi Grainne Dempsey |
| collection | DOAJ |
| description | Soft tissue is composed of cells surrounded by an extracellular matrix that is made up of a diverse array of intricately organized proteins. These distinct components work in concert to maintain homeostasis and respond to tissue damage. During tissue repair, extracellular matrix proteins and their degradation products are known to influence physiological processes such as angiogenesis and inflammation. In this study we developed a discovery platform using a decellularized extracellular matrix biomaterial to identify new chemotrophic factors derived from the extracellular matrix. An in vitro culture of RAW.264 macrophage cells with the biomaterial ovine forestomach matrix led to the identification of a novel ~12 kDa chemotactic factor, termed 'MayDay', derived from the N-terminal 31-188 sequence of decorin. The recombinant MayDay protein was shown to be a chemotactic agent for mesenchymal stromal cells in vitro and in vivo. We hypothesize that the macrophage-induced cleavage of decorin, via MMP-12, leads to the release of the chemotactic molecule MayDay, that in turn recruits cells to the site of damaged tissue. |
| first_indexed | 2024-12-13T22:23:22Z |
| format | Article |
| id | doaj.art-57a00a5221af40519a860e469351449c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T22:23:22Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-57a00a5221af40519a860e469351449c2022-12-21T23:29:17ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01157e023578410.1371/journal.pone.0235784A novel chemotactic factor derived from the extracellular matrix protein decorin recruits mesenchymal stromal cells in vitro and in vivo.Sandi Grainne DempseyChristopher Hamilton MillerJulia SchuelerRobert W F VealeDarren J DayBarnaby C H MaySoft tissue is composed of cells surrounded by an extracellular matrix that is made up of a diverse array of intricately organized proteins. These distinct components work in concert to maintain homeostasis and respond to tissue damage. During tissue repair, extracellular matrix proteins and their degradation products are known to influence physiological processes such as angiogenesis and inflammation. In this study we developed a discovery platform using a decellularized extracellular matrix biomaterial to identify new chemotrophic factors derived from the extracellular matrix. An in vitro culture of RAW.264 macrophage cells with the biomaterial ovine forestomach matrix led to the identification of a novel ~12 kDa chemotactic factor, termed 'MayDay', derived from the N-terminal 31-188 sequence of decorin. The recombinant MayDay protein was shown to be a chemotactic agent for mesenchymal stromal cells in vitro and in vivo. We hypothesize that the macrophage-induced cleavage of decorin, via MMP-12, leads to the release of the chemotactic molecule MayDay, that in turn recruits cells to the site of damaged tissue.https://doi.org/10.1371/journal.pone.0235784 |
| spellingShingle | Sandi Grainne Dempsey Christopher Hamilton Miller Julia Schueler Robert W F Veale Darren J Day Barnaby C H May A novel chemotactic factor derived from the extracellular matrix protein decorin recruits mesenchymal stromal cells in vitro and in vivo. PLoS ONE |
| title | A novel chemotactic factor derived from the extracellular matrix protein decorin recruits mesenchymal stromal cells in vitro and in vivo. |
| title_full | A novel chemotactic factor derived from the extracellular matrix protein decorin recruits mesenchymal stromal cells in vitro and in vivo. |
| title_fullStr | A novel chemotactic factor derived from the extracellular matrix protein decorin recruits mesenchymal stromal cells in vitro and in vivo. |
| title_full_unstemmed | A novel chemotactic factor derived from the extracellular matrix protein decorin recruits mesenchymal stromal cells in vitro and in vivo. |
| title_short | A novel chemotactic factor derived from the extracellular matrix protein decorin recruits mesenchymal stromal cells in vitro and in vivo. |
| title_sort | novel chemotactic factor derived from the extracellular matrix protein decorin recruits mesenchymal stromal cells in vitro and in vivo |
| url | https://doi.org/10.1371/journal.pone.0235784 |
| work_keys_str_mv | AT sandigrainnedempsey anovelchemotacticfactorderivedfromtheextracellularmatrixproteindecorinrecruitsmesenchymalstromalcellsinvitroandinvivo AT christopherhamiltonmiller anovelchemotacticfactorderivedfromtheextracellularmatrixproteindecorinrecruitsmesenchymalstromalcellsinvitroandinvivo AT juliaschueler anovelchemotacticfactorderivedfromtheextracellularmatrixproteindecorinrecruitsmesenchymalstromalcellsinvitroandinvivo AT robertwfveale anovelchemotacticfactorderivedfromtheextracellularmatrixproteindecorinrecruitsmesenchymalstromalcellsinvitroandinvivo AT darrenjday anovelchemotacticfactorderivedfromtheextracellularmatrixproteindecorinrecruitsmesenchymalstromalcellsinvitroandinvivo AT barnabychmay anovelchemotacticfactorderivedfromtheextracellularmatrixproteindecorinrecruitsmesenchymalstromalcellsinvitroandinvivo AT sandigrainnedempsey novelchemotacticfactorderivedfromtheextracellularmatrixproteindecorinrecruitsmesenchymalstromalcellsinvitroandinvivo AT christopherhamiltonmiller novelchemotacticfactorderivedfromtheextracellularmatrixproteindecorinrecruitsmesenchymalstromalcellsinvitroandinvivo AT juliaschueler novelchemotacticfactorderivedfromtheextracellularmatrixproteindecorinrecruitsmesenchymalstromalcellsinvitroandinvivo AT robertwfveale novelchemotacticfactorderivedfromtheextracellularmatrixproteindecorinrecruitsmesenchymalstromalcellsinvitroandinvivo AT darrenjday novelchemotacticfactorderivedfromtheextracellularmatrixproteindecorinrecruitsmesenchymalstromalcellsinvitroandinvivo AT barnabychmay novelchemotacticfactorderivedfromtheextracellularmatrixproteindecorinrecruitsmesenchymalstromalcellsinvitroandinvivo |