Phenotypic Characterization and Antimicrobial Susceptibility in Clinical Strains of Stenotrophomonas maltophilia
Stenotrophomonas maltophilia, previously known as Xanthomonas maltophilia and Pseudomonas maltophilia, is a Gram negative rod, and a non fermentator of glucose, recognized as a causal agent of diverse hospital infections, it also shows resistance to multiple antimicrobial agents. The objective of th...
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| Language: | English |
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Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales
2005-08-01
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| Series: | Kasmera |
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| Online Access: | https://produccioncientificaluz.org/index.php/kasmera/article/view/4756 |
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| author | Dina Antón Yasmina Araque Marcos De Donato Belkis Medina María Marcano |
| author_facet | Dina Antón Yasmina Araque Marcos De Donato Belkis Medina María Marcano |
| author_sort | Dina Antón |
| collection | DOAJ |
| description | Stenotrophomonas maltophilia, previously known as Xanthomonas maltophilia and Pseudomonas maltophilia, is a Gram negative rod, and a non fermentator of glucose, recognized as a causal agent of diverse hospital infections, it also shows resistance to multiple antimicrobial agents. The objective of the present study was to identify and to characterize phenol-type, clinical isolates of S. maltophilia, as well as to establish an in vitro activity pattern of different antimicrobials. The bacterial strains were isolatde in the Bacteriology Laboratory at the General Hospital in Cumaná, and were identified through conventional biochemical tests, as well as using the commercial galleries of API ID32GN. The MIC of the anti-microbes was determined by dilution in Mueller Hutton agar following CLSI norms. Pheno-typically, 24 strains were identified of S. maltophilia. The identification was 100% coincident with both classification techniques. The uniform pattern was maintained for the isolates except for the oxidase test, where 17 (70.83%) were positive and 7 (29.17%) negative. The results of the identification test showed the presence of 5 phenotypes in which the typical pattern of the microorganism (phenotype I, 14 isolates) prevailed. The strains showed 100% resistance to imipenem, cefepime, amikacin and nalidixico, 95.83% to cefotaxime, ceftazidime and ampicillin- sulbactam, 91,67% to ceftriaxone and ciprofloxacin, and 75.00% to piperacillin-tazobactam, all of which showed more susceptibility to trimetoprim sulfametoxazole (91.67%), levofloxacin (75%) and rifampin (95.83%) |
| first_indexed | 2024-03-12T10:42:35Z |
| format | Article |
| id | doaj.art-57a02ba20a784eec8461b80b589867ba |
| institution | Directory Open Access Journal |
| issn | 0075-5222 2477-9628 |
| language | English |
| last_indexed | 2024-03-12T10:42:35Z |
| publishDate | 2005-08-01 |
| publisher | Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales |
| record_format | Article |
| series | Kasmera |
| spelling | doaj.art-57a02ba20a784eec8461b80b589867ba2023-09-02T08:03:04ZengUniversidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y TropicalesKasmera0075-52222477-96282005-08-0133210911824303Phenotypic Characterization and Antimicrobial Susceptibility in Clinical Strains of Stenotrophomonas maltophiliaDina Antón0Yasmina Araque1Marcos De Donato2Belkis Medina3María Marcano4Postgrado en Biología Aplicada. Universidad de Oriente. Núcleo de SucreDepartamento de Bioanálisis. Universidad de Oriente. Núcleo de SucreLaboratorio de Genética Molecular. Instituto de Investigaciones en Biomedicina y Ciencias Aplicadas. Universidad de Oriente. Núcleo de Sucre.Laboratorio Bacteriología. Hospital Universitario Antonio Patricio Alcala.Laboratorio Bacteriología Hospital Universitario Antonio Patricio Alcala.Stenotrophomonas maltophilia, previously known as Xanthomonas maltophilia and Pseudomonas maltophilia, is a Gram negative rod, and a non fermentator of glucose, recognized as a causal agent of diverse hospital infections, it also shows resistance to multiple antimicrobial agents. The objective of the present study was to identify and to characterize phenol-type, clinical isolates of S. maltophilia, as well as to establish an in vitro activity pattern of different antimicrobials. The bacterial strains were isolatde in the Bacteriology Laboratory at the General Hospital in Cumaná, and were identified through conventional biochemical tests, as well as using the commercial galleries of API ID32GN. The MIC of the anti-microbes was determined by dilution in Mueller Hutton agar following CLSI norms. Pheno-typically, 24 strains were identified of S. maltophilia. The identification was 100% coincident with both classification techniques. The uniform pattern was maintained for the isolates except for the oxidase test, where 17 (70.83%) were positive and 7 (29.17%) negative. The results of the identification test showed the presence of 5 phenotypes in which the typical pattern of the microorganism (phenotype I, 14 isolates) prevailed. The strains showed 100% resistance to imipenem, cefepime, amikacin and nalidixico, 95.83% to cefotaxime, ceftazidime and ampicillin- sulbactam, 91,67% to ceftriaxone and ciprofloxacin, and 75.00% to piperacillin-tazobactam, all of which showed more susceptibility to trimetoprim sulfametoxazole (91.67%), levofloxacin (75%) and rifampin (95.83%)https://produccioncientificaluz.org/index.php/kasmera/article/view/4756S. maltophilia, Antimicrobianos, susceptibilidad, fenotipificación, infección nosocomial |
| spellingShingle | Dina Antón Yasmina Araque Marcos De Donato Belkis Medina María Marcano Phenotypic Characterization and Antimicrobial Susceptibility in Clinical Strains of Stenotrophomonas maltophilia Kasmera S. maltophilia, Antimicrobianos, susceptibilidad, fenotipificación, infección nosocomial |
| title | Phenotypic Characterization and Antimicrobial Susceptibility in Clinical Strains of Stenotrophomonas maltophilia |
| title_full | Phenotypic Characterization and Antimicrobial Susceptibility in Clinical Strains of Stenotrophomonas maltophilia |
| title_fullStr | Phenotypic Characterization and Antimicrobial Susceptibility in Clinical Strains of Stenotrophomonas maltophilia |
| title_full_unstemmed | Phenotypic Characterization and Antimicrobial Susceptibility in Clinical Strains of Stenotrophomonas maltophilia |
| title_short | Phenotypic Characterization and Antimicrobial Susceptibility in Clinical Strains of Stenotrophomonas maltophilia |
| title_sort | phenotypic characterization and antimicrobial susceptibility in clinical strains of stenotrophomonas maltophilia |
| topic | S. maltophilia, Antimicrobianos, susceptibilidad, fenotipificación, infección nosocomial |
| url | https://produccioncientificaluz.org/index.php/kasmera/article/view/4756 |
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