Chemical Characterization, Antitumor, and Immune-Enhancing Activities of Polysaccharide from <i>Sargassum pallidum</i>
Searching for natural products with antitumor and immune-enhancing activities is an important aspect of cancer research. <i>Sargassum pallidum</i> is an edible brown alga that has been used in Chinese traditional medicine for the treatment of tumors. However, the purification and applica...
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MDPI AG
2021-12-01
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| Series: | Molecules |
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| author | Yi Gao Yizhen Li Yunze Niu Hao Ju Ran Chen Bin Li Xiyun Song Lin Song |
| author_facet | Yi Gao Yizhen Li Yunze Niu Hao Ju Ran Chen Bin Li Xiyun Song Lin Song |
| author_sort | Yi Gao |
| collection | DOAJ |
| description | Searching for natural products with antitumor and immune-enhancing activities is an important aspect of cancer research. <i>Sargassum pallidum</i> is an edible brown alga that has been used in Chinese traditional medicine for the treatment of tumors. However, the purification and application of its active components are still insufficient. In the present study, the polysaccharides from <i>S. pallidum</i> (SPPs) with antitumor and immune-enhancing activities were isolated and purified, and five polysaccharide fractions (SPP-0.3, SPP-0.5, SPP-0.7, SPP-1, and SPP-2) were obtained. The ratio of total saccharides, monosaccharide composition, and sulfated contents was determined, and their structures were analyzed by Fourier transform infrared spectroscopy. Moreover, bioactivity analysis showed that all five fractions had significant antitumor activity against three types of cancer cells (A549, HepG2, and B16), and can induce cancer cell apoptosis. In addition, the results indicated that SPPs can enhance the proliferation of immune cells and improve the expression levels of serum cytokines (IL-6, IL-1β, iNOS, and TNF-α). SPP-0.7 was identified as the most active fraction and selected for further purification, and its physicochemical properties and antitumor mechanism were further analyzed. Transcriptome sequencing result showed that SPP-0.7 can significantly induce the cell apoptosis, cytokine secretion, and cellular stress response process, and inhibit the normal physiological processes of cancer cells. Overall, SPPs and SPP-0.7 may be suitable for use as potential candidate agents for cancer therapy. |
| first_indexed | 2024-03-10T03:29:11Z |
| format | Article |
| id | doaj.art-57a0594fd61d4516b06b07733b221554 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T03:29:11Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-57a0594fd61d4516b06b07733b2215542023-11-23T09:45:53ZengMDPI AGMolecules1420-30492021-12-012624755910.3390/molecules26247559Chemical Characterization, Antitumor, and Immune-Enhancing Activities of Polysaccharide from <i>Sargassum pallidum</i>Yi Gao0Yizhen Li1Yunze Niu2Hao Ju3Ran Chen4Bin Li5Xiyun Song6Lin Song7College of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Life Sciences, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Life Sciences, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Life Sciences, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Life Sciences, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Agronomy, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao 266042, ChinaSearching for natural products with antitumor and immune-enhancing activities is an important aspect of cancer research. <i>Sargassum pallidum</i> is an edible brown alga that has been used in Chinese traditional medicine for the treatment of tumors. However, the purification and application of its active components are still insufficient. In the present study, the polysaccharides from <i>S. pallidum</i> (SPPs) with antitumor and immune-enhancing activities were isolated and purified, and five polysaccharide fractions (SPP-0.3, SPP-0.5, SPP-0.7, SPP-1, and SPP-2) were obtained. The ratio of total saccharides, monosaccharide composition, and sulfated contents was determined, and their structures were analyzed by Fourier transform infrared spectroscopy. Moreover, bioactivity analysis showed that all five fractions had significant antitumor activity against three types of cancer cells (A549, HepG2, and B16), and can induce cancer cell apoptosis. In addition, the results indicated that SPPs can enhance the proliferation of immune cells and improve the expression levels of serum cytokines (IL-6, IL-1β, iNOS, and TNF-α). SPP-0.7 was identified as the most active fraction and selected for further purification, and its physicochemical properties and antitumor mechanism were further analyzed. Transcriptome sequencing result showed that SPP-0.7 can significantly induce the cell apoptosis, cytokine secretion, and cellular stress response process, and inhibit the normal physiological processes of cancer cells. Overall, SPPs and SPP-0.7 may be suitable for use as potential candidate agents for cancer therapy.https://www.mdpi.com/1420-3049/26/24/7559seaweed<i>Sargassum pallidum</i>polysaccharidesantitumor activityimmune-enhancing activity |
| spellingShingle | Yi Gao Yizhen Li Yunze Niu Hao Ju Ran Chen Bin Li Xiyun Song Lin Song Chemical Characterization, Antitumor, and Immune-Enhancing Activities of Polysaccharide from <i>Sargassum pallidum</i> Molecules seaweed <i>Sargassum pallidum</i> polysaccharides antitumor activity immune-enhancing activity |
| title | Chemical Characterization, Antitumor, and Immune-Enhancing Activities of Polysaccharide from <i>Sargassum pallidum</i> |
| title_full | Chemical Characterization, Antitumor, and Immune-Enhancing Activities of Polysaccharide from <i>Sargassum pallidum</i> |
| title_fullStr | Chemical Characterization, Antitumor, and Immune-Enhancing Activities of Polysaccharide from <i>Sargassum pallidum</i> |
| title_full_unstemmed | Chemical Characterization, Antitumor, and Immune-Enhancing Activities of Polysaccharide from <i>Sargassum pallidum</i> |
| title_short | Chemical Characterization, Antitumor, and Immune-Enhancing Activities of Polysaccharide from <i>Sargassum pallidum</i> |
| title_sort | chemical characterization antitumor and immune enhancing activities of polysaccharide from i sargassum pallidum i |
| topic | seaweed <i>Sargassum pallidum</i> polysaccharides antitumor activity immune-enhancing activity |
| url | https://www.mdpi.com/1420-3049/26/24/7559 |
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