Development of Broad Spectrum and Durable Bacterial Blight Resistant Variety through Pyramiding of Four Resistance Genes in Rice
Bacterial blight (BB) disease caused by <i>Xanthomonas oryzae</i> pv. <i>oryzae</i> is a major biotic constraint on obtaining higher grain yields in rice. Marker-assisted backcross breeding (MABB) was performed by the pyramiding of <i>Xa4</i>, <i>xa5</i&g...
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MDPI AG
2022-08-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/8/1903 |
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| author | Kartik Chandra Pradhan Elssa Pandit Shakti Prakash Mohanty Arpita Moharana Priyadarsini Sanghamitra Jitendriya Meher Binod Kumar Jena Prasanta K. Dash Lambodar Behera Pavitra Mohan Mohapatra Debendra Nath Bastia Sharat Kumar Pradhan |
| author_facet | Kartik Chandra Pradhan Elssa Pandit Shakti Prakash Mohanty Arpita Moharana Priyadarsini Sanghamitra Jitendriya Meher Binod Kumar Jena Prasanta K. Dash Lambodar Behera Pavitra Mohan Mohapatra Debendra Nath Bastia Sharat Kumar Pradhan |
| author_sort | Kartik Chandra Pradhan |
| collection | DOAJ |
| description | Bacterial blight (BB) disease caused by <i>Xanthomonas oryzae</i> pv. <i>oryzae</i> is a major biotic constraint on obtaining higher grain yields in rice. Marker-assisted backcross breeding (MABB) was performed by the pyramiding of <i>Xa4</i>, <i>xa5</i>, <i>xa13</i> and <i>Xa21</i> resistance genes in the popular variety, Ranidhan. A foreground selection in BC<sub>1</sub>F<sub>1</sub>, BC<sub>2</sub>F<sub>1</sub>, and BC<sub>3</sub>F<sub>1</sub> progenies detected all the target genes in 12, 7 and 16 progenies by using the closely linked markers from a population size of 446, 410, and 530, respectively. The BB-positive progenies carrying the target genes with a maximal similarity to the recipient parent was backcrossed in each backcross generation. A total of 1784 BC<sub>3</sub>F<sub>2</sub> seeds were obtained from the best BC<sub>3</sub>F<sub>1</sub> progeny. The screening of the BC<sub>3</sub>F<sub>2</sub> progenies for the four target genes resulted in eight plants carrying all the four target genes. A bioassay of the pyramided lines conferred very high levels of resistance to the predominant isolates of bacterial blight disease. In addition, these pyramided lines were similar to Ranidhan in 16 morpho-quality traits, namely, plant height, filled grains/panicle, panicles/plant, grain length, grain breadth, grain weight, milling, head rice recovery, kernel length after cooking, water uptake, the volume expansion ratio, gel consistency, alkali-spreading value, and the amylose content. |
| first_indexed | 2024-03-09T12:01:36Z |
| format | Article |
| id | doaj.art-57a1abed025640dd95a5470f6d299115 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T12:01:36Z |
| publishDate | 2022-08-01 |
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| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-57a1abed025640dd95a5470f6d2991152023-11-30T23:02:39ZengMDPI AGAgronomy2073-43952022-08-01128190310.3390/agronomy12081903Development of Broad Spectrum and Durable Bacterial Blight Resistant Variety through Pyramiding of Four Resistance Genes in RiceKartik Chandra Pradhan0Elssa Pandit1Shakti Prakash Mohanty2Arpita Moharana3Priyadarsini Sanghamitra4Jitendriya Meher5Binod Kumar Jena6Prasanta K. Dash7Lambodar Behera8Pavitra Mohan Mohapatra9Debendra Nath Bastia10Sharat Kumar Pradhan11College of Agriculture, Odisha University of Agriculture & Technology, Bhubaneswar 751003, IndiaDepartment of Biosciences and Biotechnology, Fakir Mohan University, Balasore 756020, IndiaICAR-National Rice Research Institute, Cuttack 753006, IndiaICAR-National Rice Research Institute, Cuttack 753006, IndiaICAR-National Rice Research Institute, Cuttack 753006, IndiaICAR-National Rice Research Institute, Cuttack 753006, IndiaKrishi Vigyan Kendra, Odisha University of Agriculture & Technology, Rayagada 765022, IndiaICAR-National Institute for Plant Biotechnology-NIPB, Pusa, New Delhi 110012, IndiaICAR-National Rice Research Institute, Cuttack 753006, IndiaCentre for Pulse Research, Odisha University of Agriculture & Technology, Berhampur 761001, IndiaCollege of Agriculture, Odisha University of Agriculture & Technology, Bhubaneswar 751003, IndiaICAR-National Rice Research Institute, Cuttack 753006, IndiaBacterial blight (BB) disease caused by <i>Xanthomonas oryzae</i> pv. <i>oryzae</i> is a major biotic constraint on obtaining higher grain yields in rice. Marker-assisted backcross breeding (MABB) was performed by the pyramiding of <i>Xa4</i>, <i>xa5</i>, <i>xa13</i> and <i>Xa21</i> resistance genes in the popular variety, Ranidhan. A foreground selection in BC<sub>1</sub>F<sub>1</sub>, BC<sub>2</sub>F<sub>1</sub>, and BC<sub>3</sub>F<sub>1</sub> progenies detected all the target genes in 12, 7 and 16 progenies by using the closely linked markers from a population size of 446, 410, and 530, respectively. The BB-positive progenies carrying the target genes with a maximal similarity to the recipient parent was backcrossed in each backcross generation. A total of 1784 BC<sub>3</sub>F<sub>2</sub> seeds were obtained from the best BC<sub>3</sub>F<sub>1</sub> progeny. The screening of the BC<sub>3</sub>F<sub>2</sub> progenies for the four target genes resulted in eight plants carrying all the four target genes. A bioassay of the pyramided lines conferred very high levels of resistance to the predominant isolates of bacterial blight disease. In addition, these pyramided lines were similar to Ranidhan in 16 morpho-quality traits, namely, plant height, filled grains/panicle, panicles/plant, grain length, grain breadth, grain weight, milling, head rice recovery, kernel length after cooking, water uptake, the volume expansion ratio, gel consistency, alkali-spreading value, and the amylose content.https://www.mdpi.com/2073-4395/12/8/1903bacterial blight diseasegene pyramidingresistance genesforeground selectionbackground selectionmarker-assisted selection |
| spellingShingle | Kartik Chandra Pradhan Elssa Pandit Shakti Prakash Mohanty Arpita Moharana Priyadarsini Sanghamitra Jitendriya Meher Binod Kumar Jena Prasanta K. Dash Lambodar Behera Pavitra Mohan Mohapatra Debendra Nath Bastia Sharat Kumar Pradhan Development of Broad Spectrum and Durable Bacterial Blight Resistant Variety through Pyramiding of Four Resistance Genes in Rice Agronomy bacterial blight disease gene pyramiding resistance genes foreground selection background selection marker-assisted selection |
| title | Development of Broad Spectrum and Durable Bacterial Blight Resistant Variety through Pyramiding of Four Resistance Genes in Rice |
| title_full | Development of Broad Spectrum and Durable Bacterial Blight Resistant Variety through Pyramiding of Four Resistance Genes in Rice |
| title_fullStr | Development of Broad Spectrum and Durable Bacterial Blight Resistant Variety through Pyramiding of Four Resistance Genes in Rice |
| title_full_unstemmed | Development of Broad Spectrum and Durable Bacterial Blight Resistant Variety through Pyramiding of Four Resistance Genes in Rice |
| title_short | Development of Broad Spectrum and Durable Bacterial Blight Resistant Variety through Pyramiding of Four Resistance Genes in Rice |
| title_sort | development of broad spectrum and durable bacterial blight resistant variety through pyramiding of four resistance genes in rice |
| topic | bacterial blight disease gene pyramiding resistance genes foreground selection background selection marker-assisted selection |
| url | https://www.mdpi.com/2073-4395/12/8/1903 |
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