16S rRNA Sequencing Analysis of the Gut Microbiota in Broiler Chickens Prophylactically Administered with Antimicrobial Agents
In poultry production, gut microbiota (GM) plays a pivotal role and influences different host functions related to the efficiency of production performances. Antimicrobial (AM) use is one of the main factors affecting GM composition and functions. Although several studies have focused their attentio...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-02-01
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| Series: | Antibiotics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6382/10/2/146 |
| _version_ | 1797416794983497728 |
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| author | Matteo Cuccato Selene Rubiola Diana Giannuzzi Elena Grego Paola Pregel Sara Divari Francesca Tiziana Cannizzo |
| author_facet | Matteo Cuccato Selene Rubiola Diana Giannuzzi Elena Grego Paola Pregel Sara Divari Francesca Tiziana Cannizzo |
| author_sort | Matteo Cuccato |
| collection | DOAJ |
| description | In poultry production, gut microbiota (GM) plays a pivotal role and influences different host functions related to the efficiency of production performances. Antimicrobial (AM) use is one of the main factors affecting GM composition and functions. Although several studies have focused their attention on the role of AMs as growth promoters in the modulation of GM in broilers, the consequences of higher AM concentrations administered during prophylactic treatments need to be better elucidated. For this purpose, 16S rRNA gene sequencing was performed to evaluate the impact of different prophylactic AM protocols on the composition and diversity of the broiler GM. Diversity analysis has shown that AM treatment significantly affects alpha diversity in ileum and beta diversity in both ileum and caecum. In ileal samples, the <i>Enterobacteriaceae</i> family has been shown to be particularly affected by AM treatments. AMs have been demonstrated to affect GM composition in broiler. These findings indicate that withdrawal periods were not enough for the restoral of the original GM. Further studies are needed for a better elucidation of the negative effects caused by an altered GM in broilers. |
| first_indexed | 2024-03-09T06:09:19Z |
| format | Article |
| id | doaj.art-57a414ca3fb34387acca996454f429c5 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T06:09:19Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-57a414ca3fb34387acca996454f429c52023-12-03T12:00:18ZengMDPI AGAntibiotics2079-63822021-02-0110214610.3390/antibiotics1002014616S rRNA Sequencing Analysis of the Gut Microbiota in Broiler Chickens Prophylactically Administered with Antimicrobial AgentsMatteo Cuccato0Selene Rubiola1Diana Giannuzzi2Elena Grego3Paola Pregel4Sara Divari5Francesca Tiziana Cannizzo6Department of Veterinary Science, University of Turin, Grugliasco, 10095 Turin, ItalyDepartment of Veterinary Science, University of Turin, Grugliasco, 10095 Turin, ItalyDepartment of Agronomy, Food, Natural Resources, Animals and Environment, University of Padua, 35020 Legnaro, ItalyDepartment of Veterinary Science, University of Turin, Grugliasco, 10095 Turin, ItalyDepartment of Veterinary Science, University of Turin, Grugliasco, 10095 Turin, ItalyDepartment of Veterinary Science, University of Turin, Grugliasco, 10095 Turin, ItalyDepartment of Veterinary Science, University of Turin, Grugliasco, 10095 Turin, ItalyIn poultry production, gut microbiota (GM) plays a pivotal role and influences different host functions related to the efficiency of production performances. Antimicrobial (AM) use is one of the main factors affecting GM composition and functions. Although several studies have focused their attention on the role of AMs as growth promoters in the modulation of GM in broilers, the consequences of higher AM concentrations administered during prophylactic treatments need to be better elucidated. For this purpose, 16S rRNA gene sequencing was performed to evaluate the impact of different prophylactic AM protocols on the composition and diversity of the broiler GM. Diversity analysis has shown that AM treatment significantly affects alpha diversity in ileum and beta diversity in both ileum and caecum. In ileal samples, the <i>Enterobacteriaceae</i> family has been shown to be particularly affected by AM treatments. AMs have been demonstrated to affect GM composition in broiler. These findings indicate that withdrawal periods were not enough for the restoral of the original GM. Further studies are needed for a better elucidation of the negative effects caused by an altered GM in broilers.https://www.mdpi.com/2079-6382/10/2/146prophylaxiscoccidiostatbroiler16S rRNA sequencingileumcaecum |
| spellingShingle | Matteo Cuccato Selene Rubiola Diana Giannuzzi Elena Grego Paola Pregel Sara Divari Francesca Tiziana Cannizzo 16S rRNA Sequencing Analysis of the Gut Microbiota in Broiler Chickens Prophylactically Administered with Antimicrobial Agents Antibiotics prophylaxis coccidiostat broiler 16S rRNA sequencing ileum caecum |
| title | 16S rRNA Sequencing Analysis of the Gut Microbiota in Broiler Chickens Prophylactically Administered with Antimicrobial Agents |
| title_full | 16S rRNA Sequencing Analysis of the Gut Microbiota in Broiler Chickens Prophylactically Administered with Antimicrobial Agents |
| title_fullStr | 16S rRNA Sequencing Analysis of the Gut Microbiota in Broiler Chickens Prophylactically Administered with Antimicrobial Agents |
| title_full_unstemmed | 16S rRNA Sequencing Analysis of the Gut Microbiota in Broiler Chickens Prophylactically Administered with Antimicrobial Agents |
| title_short | 16S rRNA Sequencing Analysis of the Gut Microbiota in Broiler Chickens Prophylactically Administered with Antimicrobial Agents |
| title_sort | 16s rrna sequencing analysis of the gut microbiota in broiler chickens prophylactically administered with antimicrobial agents |
| topic | prophylaxis coccidiostat broiler 16S rRNA sequencing ileum caecum |
| url | https://www.mdpi.com/2079-6382/10/2/146 |
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