Moderation effects of food intake on the relationship between urinary microbiota and urinary interleukin-8 in female type 2 diabetic patients
Background Our previous study demonstrated that the composition of the urinary microbiota in female patients with type 2 diabetes mellitus (T2DM) was correlated with the concentration of urinary interleukin (IL)-8. As the composition of urine is mainly determined by diet, diet might mediate the corr...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PeerJ Inc.
2020-01-01
|
| Series: | PeerJ |
| Subjects: | |
| Online Access: | https://peerj.com/articles/8481.pdf |
| _version_ | 1797424248031019008 |
|---|---|
| author | Fengping Liu Zongxin Ling Chulei Tang Fendi Yi Yong Q. Chen |
| author_facet | Fengping Liu Zongxin Ling Chulei Tang Fendi Yi Yong Q. Chen |
| author_sort | Fengping Liu |
| collection | DOAJ |
| description | Background Our previous study demonstrated that the composition of the urinary microbiota in female patients with type 2 diabetes mellitus (T2DM) was correlated with the concentration of urinary interleukin (IL)-8. As the composition of urine is mainly determined by diet, diet might mediate the correlation. Methods Seventy female T2DM patients and 70 healthy controls (HCs) were recruited. Midstream urine was used for the urine specimens. Urinary IL-8 was determined by enzyme-linked immunosorbent assay. A Chinese Food Frequency Questionnaire was used to collect food intake data. The independent variables in the hierarchical regression analysis were the relative abundances of the bacterial genera and species that were significantly different between the T2DM and HCs and between the T2DM patients with and without detectable urinary IL-8, and the bacterial genera associated with IL-8 concentration in the multiple regression model reported in our previous research. IL-8 concentration was the dependent variable, and nutrient intakes were moderator variables. Results Fiber and vitamin B3 and E intake exerted enhancing effects, and water intake exerted a buffering effect, on the positive relationship between the relative abundance of Ruminococcus and IL-8 concentration (p < 0.05). Cholesterol and magnesium intake exerted enhancing effects on the positive relationship between the relative abundance of Comamonas and IL-8 concentration (p < 0.05). Conclusion Modulating T2DM patients’ dietary patterns may prevent bladder inflammation. |
| first_indexed | 2024-03-09T07:59:33Z |
| format | Article |
| id | doaj.art-57a51eec05f0426783585d34dd94d160 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:59:33Z |
| publishDate | 2020-01-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-57a51eec05f0426783585d34dd94d1602023-12-03T00:48:12ZengPeerJ Inc.PeerJ2167-83592020-01-018e848110.7717/peerj.8481Moderation effects of food intake on the relationship between urinary microbiota and urinary interleukin-8 in female type 2 diabetic patientsFengping Liu0Zongxin Ling1Chulei Tang2Fendi Yi3Yong Q. Chen4Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu, ChinaCollaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, ChinaXiangya Nursing School, Central South University, Changsha, ChinaEndocrinology Department, The Affiliated Yancheng Hospital of Southeast University Medical College, Yancheng, ChinaWuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu, ChinaBackground Our previous study demonstrated that the composition of the urinary microbiota in female patients with type 2 diabetes mellitus (T2DM) was correlated with the concentration of urinary interleukin (IL)-8. As the composition of urine is mainly determined by diet, diet might mediate the correlation. Methods Seventy female T2DM patients and 70 healthy controls (HCs) were recruited. Midstream urine was used for the urine specimens. Urinary IL-8 was determined by enzyme-linked immunosorbent assay. A Chinese Food Frequency Questionnaire was used to collect food intake data. The independent variables in the hierarchical regression analysis were the relative abundances of the bacterial genera and species that were significantly different between the T2DM and HCs and between the T2DM patients with and without detectable urinary IL-8, and the bacterial genera associated with IL-8 concentration in the multiple regression model reported in our previous research. IL-8 concentration was the dependent variable, and nutrient intakes were moderator variables. Results Fiber and vitamin B3 and E intake exerted enhancing effects, and water intake exerted a buffering effect, on the positive relationship between the relative abundance of Ruminococcus and IL-8 concentration (p < 0.05). Cholesterol and magnesium intake exerted enhancing effects on the positive relationship between the relative abundance of Comamonas and IL-8 concentration (p < 0.05). Conclusion Modulating T2DM patients’ dietary patterns may prevent bladder inflammation.https://peerj.com/articles/8481.pdfFood intakeType 2 diabetes mellitusInterleukin-8Moderating effectRuminococcusUrinary microbiota |
| spellingShingle | Fengping Liu Zongxin Ling Chulei Tang Fendi Yi Yong Q. Chen Moderation effects of food intake on the relationship between urinary microbiota and urinary interleukin-8 in female type 2 diabetic patients PeerJ Food intake Type 2 diabetes mellitus Interleukin-8 Moderating effect Ruminococcus Urinary microbiota |
| title | Moderation effects of food intake on the relationship between urinary microbiota and urinary interleukin-8 in female type 2 diabetic patients |
| title_full | Moderation effects of food intake on the relationship between urinary microbiota and urinary interleukin-8 in female type 2 diabetic patients |
| title_fullStr | Moderation effects of food intake on the relationship between urinary microbiota and urinary interleukin-8 in female type 2 diabetic patients |
| title_full_unstemmed | Moderation effects of food intake on the relationship between urinary microbiota and urinary interleukin-8 in female type 2 diabetic patients |
| title_short | Moderation effects of food intake on the relationship between urinary microbiota and urinary interleukin-8 in female type 2 diabetic patients |
| title_sort | moderation effects of food intake on the relationship between urinary microbiota and urinary interleukin 8 in female type 2 diabetic patients |
| topic | Food intake Type 2 diabetes mellitus Interleukin-8 Moderating effect Ruminococcus Urinary microbiota |
| url | https://peerj.com/articles/8481.pdf |
| work_keys_str_mv | AT fengpingliu moderationeffectsoffoodintakeontherelationshipbetweenurinarymicrobiotaandurinaryinterleukin8infemaletype2diabeticpatients AT zongxinling moderationeffectsoffoodintakeontherelationshipbetweenurinarymicrobiotaandurinaryinterleukin8infemaletype2diabeticpatients AT chuleitang moderationeffectsoffoodintakeontherelationshipbetweenurinarymicrobiotaandurinaryinterleukin8infemaletype2diabeticpatients AT fendiyi moderationeffectsoffoodintakeontherelationshipbetweenurinarymicrobiotaandurinaryinterleukin8infemaletype2diabeticpatients AT yongqchen moderationeffectsoffoodintakeontherelationshipbetweenurinarymicrobiotaandurinaryinterleukin8infemaletype2diabeticpatients |