Fast tracking alien gene discovery by molecular markers in a late flowering Chinese cabbage-cabbage translocation line ‘AT7–4’
Flowering time is an important agronomic trait of Chinese cabbage with late flowering being a primary breeding objective. In our previous work, we obtained Chinese cabbage-cabbage translocation lines that contained several beneficial cabbage genes. Cabbage-specific molecular markers show that these...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2023-02-01
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| Series: | Horticultural Plant Journal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468014121001436 |
| _version_ | 1811168403638976512 |
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| author | Hui Li Aixia Gu Daling Feng Na Li Rui Yang Xinpei Zhang Shuangxia Luo Umer Karamat Qianyun Wang Shuxin Xuan Xueping Chen Yin Lu Yanhua Wang Shuxing Shen Jianjun Zhao |
| author_facet | Hui Li Aixia Gu Daling Feng Na Li Rui Yang Xinpei Zhang Shuangxia Luo Umer Karamat Qianyun Wang Shuxin Xuan Xueping Chen Yin Lu Yanhua Wang Shuxing Shen Jianjun Zhao |
| author_sort | Hui Li |
| collection | DOAJ |
| description | Flowering time is an important agronomic trait of Chinese cabbage with late flowering being a primary breeding objective. In our previous work, we obtained Chinese cabbage-cabbage translocation lines that contained several beneficial cabbage genes. Cabbage-specific molecular markers show that these genes were coming from chromosome C01 of cabbage. In this study, we investigated the inheritance of flowering time in a couple of translocation lines and analyzed the transmission rate of molecular markers in the offspring. Consequently, we obtained the late flowering Chinese cabbage-cabbage translocation line ‘AT7–4’ in which the flowering time was later than that of ‘85–1’ by about 7 days under 4-week vernalization. Based on previous studies of the genomes of Chinese cabbage and cabbage, we located the cabbage-specific molecular markers that were closely linked at the top of the chromosome A01 in the F2 mapping population generated by self-crossing F1s derived from a cross between the translocation line ‘AT7–4’ and Chinese cabbage ‘14–36’. Five flowering-related genes in the alien fragment were found by functional annotation and their molecular markers were developed. This study lays the foundation for the future improvement of Chinese cabbage varieties using A-C translocation lines. |
| first_indexed | 2024-04-10T16:26:48Z |
| format | Article |
| id | doaj.art-57aaa4fe548c4d07983ec698fbe1bbf6 |
| institution | Directory Open Access Journal |
| issn | 2468-0141 |
| language | English |
| last_indexed | 2024-04-10T16:26:48Z |
| publishDate | 2023-02-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Horticultural Plant Journal |
| spelling | doaj.art-57aaa4fe548c4d07983ec698fbe1bbf62023-02-09T04:14:40ZengKeAi Communications Co., Ltd.Horticultural Plant Journal2468-01412023-02-01918997Fast tracking alien gene discovery by molecular markers in a late flowering Chinese cabbage-cabbage translocation line ‘AT7–4’Hui Li0Aixia Gu1Daling Feng2Na Li3Rui Yang4Xinpei Zhang5Shuangxia Luo6Umer Karamat7Qianyun Wang8Shuxin Xuan9Xueping Chen10Yin Lu11Yanhua Wang12Shuxing Shen13Jianjun Zhao14State Key Laboratory of North China Crop Improvement and Regulation, Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, Baoding, Hebei 071000, China; College of Horticulture, Hebei Agricultural University, Baoding, Hebei 071000, ChinaState Key Laboratory of North China Crop Improvement and Regulation, Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, Baoding, Hebei 071000, China; College of Horticulture, Hebei Agricultural University, Baoding, Hebei 071000, ChinaState Key Laboratory of North China Crop Improvement and Regulation, Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, Baoding, Hebei 071000, China; College of Life Science, Hebei Agricultural University, Baoding, Hebei 071000, ChinaState Key Laboratory of North China Crop Improvement and Regulation, Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, Baoding, Hebei 071000, China; College of Horticulture, Hebei Agricultural University, Baoding, Hebei 071000, ChinaState Key Laboratory of North China Crop Improvement and Regulation, Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, Baoding, Hebei 071000, China; College of Horticulture, Hebei Agricultural University, Baoding, Hebei 071000, ChinaState Key Laboratory of North China Crop Improvement and Regulation, Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, Baoding, Hebei 071000, China; College of Horticulture, Hebei Agricultural University, Baoding, Hebei 071000, ChinaState Key Laboratory of North China Crop Improvement and Regulation, Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, Baoding, Hebei 071000, China; College of Horticulture, Hebei Agricultural University, Baoding, Hebei 071000, ChinaState Key Laboratory of North China Crop Improvement and Regulation, Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, Baoding, Hebei 071000, China; College of Horticulture, Hebei Agricultural University, Baoding, Hebei 071000, ChinaState Key Laboratory of North China Crop Improvement and Regulation, Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, Baoding, Hebei 071000, China; College of Horticulture, Hebei Agricultural University, Baoding, Hebei 071000, ChinaState Key Laboratory of North China Crop Improvement and Regulation, Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, Baoding, Hebei 071000, China; College of Horticulture, Hebei Agricultural University, Baoding, Hebei 071000, ChinaState Key Laboratory of North China Crop Improvement and Regulation, Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, Baoding, Hebei 071000, China; College of Horticulture, Hebei Agricultural University, Baoding, Hebei 071000, ChinaState Key Laboratory of North China Crop Improvement and Regulation, Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, Baoding, Hebei 071000, China; College of Horticulture, Hebei Agricultural University, Baoding, Hebei 071000, ChinaState Key Laboratory of North China Crop Improvement and Regulation, Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, Baoding, Hebei 071000, China; College of Horticulture, Hebei Agricultural University, Baoding, Hebei 071000, China; Corresponding authors.State Key Laboratory of North China Crop Improvement and Regulation, Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, Baoding, Hebei 071000, China; College of Horticulture, Hebei Agricultural University, Baoding, Hebei 071000, China; Corresponding authors.State Key Laboratory of North China Crop Improvement and Regulation, Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, Baoding, Hebei 071000, China; College of Horticulture, Hebei Agricultural University, Baoding, Hebei 071000, China; Corresponding authors.Flowering time is an important agronomic trait of Chinese cabbage with late flowering being a primary breeding objective. In our previous work, we obtained Chinese cabbage-cabbage translocation lines that contained several beneficial cabbage genes. Cabbage-specific molecular markers show that these genes were coming from chromosome C01 of cabbage. In this study, we investigated the inheritance of flowering time in a couple of translocation lines and analyzed the transmission rate of molecular markers in the offspring. Consequently, we obtained the late flowering Chinese cabbage-cabbage translocation line ‘AT7–4’ in which the flowering time was later than that of ‘85–1’ by about 7 days under 4-week vernalization. Based on previous studies of the genomes of Chinese cabbage and cabbage, we located the cabbage-specific molecular markers that were closely linked at the top of the chromosome A01 in the F2 mapping population generated by self-crossing F1s derived from a cross between the translocation line ‘AT7–4’ and Chinese cabbage ‘14–36’. Five flowering-related genes in the alien fragment were found by functional annotation and their molecular markers were developed. This study lays the foundation for the future improvement of Chinese cabbage varieties using A-C translocation lines.http://www.sciencedirect.com/science/article/pii/S2468014121001436Chinese cabbageTranslocation lineAlien geneMolecular markerLate flowering |
| spellingShingle | Hui Li Aixia Gu Daling Feng Na Li Rui Yang Xinpei Zhang Shuangxia Luo Umer Karamat Qianyun Wang Shuxin Xuan Xueping Chen Yin Lu Yanhua Wang Shuxing Shen Jianjun Zhao Fast tracking alien gene discovery by molecular markers in a late flowering Chinese cabbage-cabbage translocation line ‘AT7–4’ Horticultural Plant Journal Chinese cabbage Translocation line Alien gene Molecular marker Late flowering |
| title | Fast tracking alien gene discovery by molecular markers in a late flowering Chinese cabbage-cabbage translocation line ‘AT7–4’ |
| title_full | Fast tracking alien gene discovery by molecular markers in a late flowering Chinese cabbage-cabbage translocation line ‘AT7–4’ |
| title_fullStr | Fast tracking alien gene discovery by molecular markers in a late flowering Chinese cabbage-cabbage translocation line ‘AT7–4’ |
| title_full_unstemmed | Fast tracking alien gene discovery by molecular markers in a late flowering Chinese cabbage-cabbage translocation line ‘AT7–4’ |
| title_short | Fast tracking alien gene discovery by molecular markers in a late flowering Chinese cabbage-cabbage translocation line ‘AT7–4’ |
| title_sort | fast tracking alien gene discovery by molecular markers in a late flowering chinese cabbage cabbage translocation line at7 4 |
| topic | Chinese cabbage Translocation line Alien gene Molecular marker Late flowering |
| url | http://www.sciencedirect.com/science/article/pii/S2468014121001436 |
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