Over-expression of heparanase promotes proliferation and migration of gallbladder cancer cell line GBC-SD
Objective To investigate the effect of heparanase over-expression on the biological function of gallbladder carcinoma cell line GBC-SD. Methods The heparanase over-expression plasmid vector was constructed and transfected into GBC-SD cells by liposome method. The transfection efficiency was verified...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2020-02-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a190883.pdf |
| _version_ | 1797365716128628736 |
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| author | LIU Zi-xiang, ZHOU Shao-bo, ZHANG Zi-yan |
| author_facet | LIU Zi-xiang, ZHOU Shao-bo, ZHANG Zi-yan |
| author_sort | LIU Zi-xiang, ZHOU Shao-bo, ZHANG Zi-yan |
| collection | DOAJ |
| description | Objective To investigate the effect of heparanase over-expression on the biological function of gallbladder carcinoma cell line GBC-SD. Methods The heparanase over-expression plasmid vector was constructed and transfected into GBC-SD cells by liposome method. The transfection efficiency was verified by RT-qPCR and Western blot. CCK-8 assay, flow cytometry and scratch healing experiment were used to detect the cell proliferation, apoptosis and migration.The expression of syndecan-1, FGF-2 and E-cadherin protein was detected by Western blot. Results The expression of heparanase mRNA(P＜0.01) and protein(P＜0.05) in gallbladder cancer cell line GBC-SD was significantly increased after transfection.Compared with the control group,the over-expression of heparanase significantly up-regulated the proliferation and migration ability of GBC-SD cells in vitro(P＜0.05), down-regulated the level of apoptosis(P＜0.05),and increased the expression of FGF-2 protein, while decreasing the expression of syndecan-1(P＜0.01) and E-cadherin(P＜0.05). Conclusions Over-expression of heparanase promotes the proliferation and migration of gallbladder cancer cell line GBC-SD, and the mechanism may be related to its regulation of E-cadherin through FGF-2. |
| first_indexed | 2024-03-08T16:52:55Z |
| format | Article |
| id | doaj.art-57aad1babff0460ba4557a5694b27626 |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T16:52:55Z |
| publishDate | 2020-02-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-57aad1babff0460ba4557a5694b276262024-01-05T03:14:04ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252020-02-01402198202Over-expression of heparanase promotes proliferation and migration of gallbladder cancer cell line GBC-SDLIU Zi-xiang, ZHOU Shao-bo, ZHANG Zi-yan0Department of General Surgery, the Second Affiliated Hospital of Bengbu Medical College,Bengbu 233000,ChinaObjective To investigate the effect of heparanase over-expression on the biological function of gallbladder carcinoma cell line GBC-SD. Methods The heparanase over-expression plasmid vector was constructed and transfected into GBC-SD cells by liposome method. The transfection efficiency was verified by RT-qPCR and Western blot. CCK-8 assay, flow cytometry and scratch healing experiment were used to detect the cell proliferation, apoptosis and migration.The expression of syndecan-1, FGF-2 and E-cadherin protein was detected by Western blot. Results The expression of heparanase mRNA(P＜0.01) and protein(P＜0.05) in gallbladder cancer cell line GBC-SD was significantly increased after transfection.Compared with the control group,the over-expression of heparanase significantly up-regulated the proliferation and migration ability of GBC-SD cells in vitro(P＜0.05), down-regulated the level of apoptosis(P＜0.05),and increased the expression of FGF-2 protein, while decreasing the expression of syndecan-1(P＜0.01) and E-cadherin(P＜0.05). Conclusions Over-expression of heparanase promotes the proliferation and migration of gallbladder cancer cell line GBC-SD, and the mechanism may be related to its regulation of E-cadherin through FGF-2.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a190883.pdfgallbladder carcinoma|heparanase|proliferation|apoptosis|migration |
| spellingShingle | LIU Zi-xiang, ZHOU Shao-bo, ZHANG Zi-yan Over-expression of heparanase promotes proliferation and migration of gallbladder cancer cell line GBC-SD Jichu yixue yu linchuang gallbladder carcinoma|heparanase|proliferation|apoptosis|migration |
| title | Over-expression of heparanase promotes proliferation and migration of gallbladder cancer cell line GBC-SD |
| title_full | Over-expression of heparanase promotes proliferation and migration of gallbladder cancer cell line GBC-SD |
| title_fullStr | Over-expression of heparanase promotes proliferation and migration of gallbladder cancer cell line GBC-SD |
| title_full_unstemmed | Over-expression of heparanase promotes proliferation and migration of gallbladder cancer cell line GBC-SD |
| title_short | Over-expression of heparanase promotes proliferation and migration of gallbladder cancer cell line GBC-SD |
| title_sort | over expression of heparanase promotes proliferation and migration of gallbladder cancer cell line gbc sd |
| topic | gallbladder carcinoma|heparanase|proliferation|apoptosis|migration |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a190883.pdf |
| work_keys_str_mv | AT liuzixiangzhoushaobozhangziyan overexpressionofheparanasepromotesproliferationandmigrationofgallbladdercancercelllinegbcsd |