OxyR Positively Influences Phaseolotoxin Synthesis and Pyoverdin Production in <i>Pseudomonas savastanoi</i> pv. <i>phaseolicola</i> NPS3121
Phaseolotoxin is a major virulence factor of the bean pathogen bacterium <i>P. savastanoi</i> pv. <i>phaseolicola</i>. This toxin plays a key role in the development of the halo blight disease in bean plants. So far, the signal transduction pathways involved in the synthesis...
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MDPI AG
2022-10-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/11/2123 |
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| author | Jackeline Lizzeta Arvizu-Gómez Alejandro Hernández-Morales Rafael Arnulfo Juárez-Navarro Juan Diego Paredes-Tadeo Juan Campos-Guillén Juan Ramiro Pacheco-Aguilar Abril Bernardette Martínez-Rizo Christian González-Reyes |
| author_facet | Jackeline Lizzeta Arvizu-Gómez Alejandro Hernández-Morales Rafael Arnulfo Juárez-Navarro Juan Diego Paredes-Tadeo Juan Campos-Guillén Juan Ramiro Pacheco-Aguilar Abril Bernardette Martínez-Rizo Christian González-Reyes |
| author_sort | Jackeline Lizzeta Arvizu-Gómez |
| collection | DOAJ |
| description | Phaseolotoxin is a major virulence factor of the bean pathogen bacterium <i>P. savastanoi</i> pv. <i>phaseolicola</i>. This toxin plays a key role in the development of the halo blight disease in bean plants. So far, the signal transduction pathways involved in the synthesis of phaseolotoxin have not been elucidated. The influence of regulation mechanisms related to the oxidative stress response, in particular the OxyR protein, it has been suggested to be involved in this process.. In this study we evaluated the role of OxyR in <i>P. savastanoi</i> pv. <i>phaseolicola</i>, mainly compared to the synthesis of phaseolotoxin and the virulence of this phytopathogen. Generation of the <i>oxyR-mutant</i>, pathogenicity and virulence tests, and analyses of gene expression by RT-PCR assays were performed. The results showed that OxyR exerts an effect on the synthesis of phaseolotoxin and positively influences the expression of the Pht and Pbo cluster genes. Likewise, OxyR influences the production of pyoverdine by the control of the expression of the genes encoding the PvdS sigma factor, involved in the synthesis of this pigment. This study is the first report on members of the OxyR regulon of <i>P. savastanoi</i> pv. <i>phaseolicola</i> NPS3121. |
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| language | English |
| last_indexed | 2024-03-09T18:49:51Z |
| publishDate | 2022-10-01 |
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| series | Microorganisms |
| spelling | doaj.art-57ace0ff78104c0cb1a98c5538f4c5d92023-11-24T05:56:09ZengMDPI AGMicroorganisms2076-26072022-10-011011212310.3390/microorganisms10112123OxyR Positively Influences Phaseolotoxin Synthesis and Pyoverdin Production in <i>Pseudomonas savastanoi</i> pv. <i>phaseolicola</i> NPS3121Jackeline Lizzeta Arvizu-Gómez0Alejandro Hernández-Morales1Rafael Arnulfo Juárez-Navarro2Juan Diego Paredes-Tadeo3Juan Campos-Guillén4Juan Ramiro Pacheco-Aguilar5Abril Bernardette Martínez-Rizo6Christian González-Reyes7Secretaría de Investigación y Posgrado, Centro Nayarita de Innovación y Transferencia de Tecnología (CENITT), Universidad Autónoma de Nayarit, Tepic 63000, MexicoFacultad de Estudios Profesionales Zona Huasteca, Universidad Autónoma de San Luis Potosí, San Luis Potosí 79060, MexicoUnidad Académica de Ciencias Químico Biológico y Farmacéuticas, Universidad Autónoma de Nayarit, Tepic 63000, MexicoUnidad Académica de Ciencias Químico Biológico y Farmacéuticas, Universidad Autónoma de Nayarit, Tepic 63000, MexicoFacultad de Química, Universidad Autónoma de Querétaro, Santiago de Querétaro 76010, MexicoFacultad de Química, Universidad Autónoma de Querétaro, Santiago de Querétaro 76010, MexicoUnidad Académica de Medicina, Universidad Autónoma de Nayarit, Tepic 63000, MexicoUnidad Académica de Ciencias Químico Biológico y Farmacéuticas, Universidad Autónoma de Nayarit, Tepic 63000, MexicoPhaseolotoxin is a major virulence factor of the bean pathogen bacterium <i>P. savastanoi</i> pv. <i>phaseolicola</i>. This toxin plays a key role in the development of the halo blight disease in bean plants. So far, the signal transduction pathways involved in the synthesis of phaseolotoxin have not been elucidated. The influence of regulation mechanisms related to the oxidative stress response, in particular the OxyR protein, it has been suggested to be involved in this process.. In this study we evaluated the role of OxyR in <i>P. savastanoi</i> pv. <i>phaseolicola</i>, mainly compared to the synthesis of phaseolotoxin and the virulence of this phytopathogen. Generation of the <i>oxyR-mutant</i>, pathogenicity and virulence tests, and analyses of gene expression by RT-PCR assays were performed. The results showed that OxyR exerts an effect on the synthesis of phaseolotoxin and positively influences the expression of the Pht and Pbo cluster genes. Likewise, OxyR influences the production of pyoverdine by the control of the expression of the genes encoding the PvdS sigma factor, involved in the synthesis of this pigment. This study is the first report on members of the OxyR regulon of <i>P. savastanoi</i> pv. <i>phaseolicola</i> NPS3121.https://www.mdpi.com/2076-2607/10/11/2123<i>P. savastanoi</i> pv. <i>phaseolicola</i>OxyR regulonoxidative stressphaseolotoxinpyoverdine |
| spellingShingle | Jackeline Lizzeta Arvizu-Gómez Alejandro Hernández-Morales Rafael Arnulfo Juárez-Navarro Juan Diego Paredes-Tadeo Juan Campos-Guillén Juan Ramiro Pacheco-Aguilar Abril Bernardette Martínez-Rizo Christian González-Reyes OxyR Positively Influences Phaseolotoxin Synthesis and Pyoverdin Production in <i>Pseudomonas savastanoi</i> pv. <i>phaseolicola</i> NPS3121 Microorganisms <i>P. savastanoi</i> pv. <i>phaseolicola</i> OxyR regulon oxidative stress phaseolotoxin pyoverdine |
| title | OxyR Positively Influences Phaseolotoxin Synthesis and Pyoverdin Production in <i>Pseudomonas savastanoi</i> pv. <i>phaseolicola</i> NPS3121 |
| title_full | OxyR Positively Influences Phaseolotoxin Synthesis and Pyoverdin Production in <i>Pseudomonas savastanoi</i> pv. <i>phaseolicola</i> NPS3121 |
| title_fullStr | OxyR Positively Influences Phaseolotoxin Synthesis and Pyoverdin Production in <i>Pseudomonas savastanoi</i> pv. <i>phaseolicola</i> NPS3121 |
| title_full_unstemmed | OxyR Positively Influences Phaseolotoxin Synthesis and Pyoverdin Production in <i>Pseudomonas savastanoi</i> pv. <i>phaseolicola</i> NPS3121 |
| title_short | OxyR Positively Influences Phaseolotoxin Synthesis and Pyoverdin Production in <i>Pseudomonas savastanoi</i> pv. <i>phaseolicola</i> NPS3121 |
| title_sort | oxyr positively influences phaseolotoxin synthesis and pyoverdin production in i pseudomonas savastanoi i pv i phaseolicola i nps3121 |
| topic | <i>P. savastanoi</i> pv. <i>phaseolicola</i> OxyR regulon oxidative stress phaseolotoxin pyoverdine |
| url | https://www.mdpi.com/2076-2607/10/11/2123 |
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