Detection of Bartonella henselae DNA in the blood of patients with livedoid vasculopathy
Abstract Background: Livedoid vasculopathy (LV) manifests as ulcers and atrophic white scars on the lower extremities. The main known etiopathogenesis is hypercoagulability with thrombus formation, followed by inflammation. Thrombophilia, collagen and myeloproliferative diseases may induce LV, but...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Dermatologia
2023-08-01
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| Series: | Anais Brasileiros de Dermatologia |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0365-05962023000400472&tlng=en |
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| author | Marina Rovani Drummond Luciene Silva dos Santos Lais Bomediano Souza Gabriela Nero Mitsuushi Maria Letícia Cintra Andrea Fernandes Eloy da Costa França Elemir Macedo de Souza Paulo Eduardo Neves Ferreira Velho |
| author_facet | Marina Rovani Drummond Luciene Silva dos Santos Lais Bomediano Souza Gabriela Nero Mitsuushi Maria Letícia Cintra Andrea Fernandes Eloy da Costa França Elemir Macedo de Souza Paulo Eduardo Neves Ferreira Velho |
| author_sort | Marina Rovani Drummond |
| collection | DOAJ |
| description | Abstract Background: Livedoid vasculopathy (LV) manifests as ulcers and atrophic white scars on the lower extremities. The main known etiopathogenesis is hypercoagulability with thrombus formation, followed by inflammation. Thrombophilia, collagen and myeloproliferative diseases may induce LV, but the idiopathic (primary) form predominates. Bartonella spp. may cause intra-endothelial infection and skin manifestations caused by these bacteria may be diverse, including leukocytoclastic vasculitis and ulcers. Objective: The aim of this study was to investigate the presence of bacteremia by Bartonella spp. in patients with difficult-to-control chronic ulcers diagnosed as primary LV. Methods: Questionnaires and molecular tests (conventional PCR, nested PCR and real-time PCR) were applied and liquid and solid cultures were performed in the blood samples and blood clot of 16 LV patients and 32 healthy volunteers. Results: Bartonella henselae DNA was detected in 25% of LV patients and in 12.5% of control subjects but failed to reach statistically significant differences (p = 0.413). Study limitations: Due to the rarity of primary LV, the number of patients studied was small and there was greater exposure of the control group to risk factors for Bartonella spp. infection. Conclusion: Although there was no statistically significant difference between the groups, the DNA of B. henselae was detected in one of every four patients, which reinforces the need to investigate Bartonella spp. in patients with primary LV. |
| first_indexed | 2024-03-12T14:53:36Z |
| format | Article |
| id | doaj.art-57af19a12e4444d5aa39ff34ba1b2354 |
| institution | Directory Open Access Journal |
| issn | 0365-0596 |
| language | English |
| last_indexed | 2024-03-12T14:53:36Z |
| publishDate | 2023-08-01 |
| publisher | Sociedade Brasileira de Dermatologia |
| record_format | Article |
| series | Anais Brasileiros de Dermatologia |
| spelling | doaj.art-57af19a12e4444d5aa39ff34ba1b23542023-08-15T07:40:54ZengSociedade Brasileira de DermatologiaAnais Brasileiros de Dermatologia0365-05962023-08-0198447247910.1016/j.abd.2022.07.007Detection of Bartonella henselae DNA in the blood of patients with livedoid vasculopathyMarina Rovani Drummondhttps://orcid.org/0000-0001-6849-608XLuciene Silva dos Santoshttps://orcid.org/0000-0001-7867-4118Lais Bomediano Souzahttps://orcid.org/0000-0002-8980-0690Gabriela Nero Mitsuushihttps://orcid.org/0000-0003-1941-525XMaria Letícia CintraAndrea Fernandes Eloy da Costa Françahttps://orcid.org/0000-0003-1657-4570Elemir Macedo de Souzahttps://orcid.org/0000-0002-3944-0711Paulo Eduardo Neves Ferreira Velhohttps://orcid.org/0000-0001-7901-2351Abstract Background: Livedoid vasculopathy (LV) manifests as ulcers and atrophic white scars on the lower extremities. The main known etiopathogenesis is hypercoagulability with thrombus formation, followed by inflammation. Thrombophilia, collagen and myeloproliferative diseases may induce LV, but the idiopathic (primary) form predominates. Bartonella spp. may cause intra-endothelial infection and skin manifestations caused by these bacteria may be diverse, including leukocytoclastic vasculitis and ulcers. Objective: The aim of this study was to investigate the presence of bacteremia by Bartonella spp. in patients with difficult-to-control chronic ulcers diagnosed as primary LV. Methods: Questionnaires and molecular tests (conventional PCR, nested PCR and real-time PCR) were applied and liquid and solid cultures were performed in the blood samples and blood clot of 16 LV patients and 32 healthy volunteers. Results: Bartonella henselae DNA was detected in 25% of LV patients and in 12.5% of control subjects but failed to reach statistically significant differences (p = 0.413). Study limitations: Due to the rarity of primary LV, the number of patients studied was small and there was greater exposure of the control group to risk factors for Bartonella spp. infection. Conclusion: Although there was no statistically significant difference between the groups, the DNA of B. henselae was detected in one of every four patients, which reinforces the need to investigate Bartonella spp. in patients with primary LV.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0365-05962023000400472&tlng=enBartonellaLivedoid vasculopathySkin ulcer |
| spellingShingle | Marina Rovani Drummond Luciene Silva dos Santos Lais Bomediano Souza Gabriela Nero Mitsuushi Maria Letícia Cintra Andrea Fernandes Eloy da Costa França Elemir Macedo de Souza Paulo Eduardo Neves Ferreira Velho Detection of Bartonella henselae DNA in the blood of patients with livedoid vasculopathy Anais Brasileiros de Dermatologia Bartonella Livedoid vasculopathy Skin ulcer |
| title | Detection of Bartonella henselae DNA in the blood of patients with livedoid vasculopathy |
| title_full | Detection of Bartonella henselae DNA in the blood of patients with livedoid vasculopathy |
| title_fullStr | Detection of Bartonella henselae DNA in the blood of patients with livedoid vasculopathy |
| title_full_unstemmed | Detection of Bartonella henselae DNA in the blood of patients with livedoid vasculopathy |
| title_short | Detection of Bartonella henselae DNA in the blood of patients with livedoid vasculopathy |
| title_sort | detection of bartonella henselae dna in the blood of patients with livedoid vasculopathy |
| topic | Bartonella Livedoid vasculopathy Skin ulcer |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0365-05962023000400472&tlng=en |
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