Lateral interactions between CD276 and CD147 are essential for stemness in breast cancer: a novel insight from proximal proteome analysis
Abstract Oncogenic cell-surface membrane proteins contribute to the phenotypic and functional characteristics of cancer stem cells (CSCs). We employed a proximity-labeling proteomic approach to quantitatively analyze the cell-surface membrane proteins in close proximity to CD147 in CSCs. Furthermore...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-08-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-41416-7 |
| _version_ | 1797576900977098752 |
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| author | Yu Ri Seo Junghyeon Lee Han Suk Ryu EunHee G. Kim So Hyun Kim Jieun Jeong Hyeryeon Jung YeoJin Jung Han Byeol Kim Yeon Hui Jo Yeong Dong Kim Min-Sun Jin Yong Yook Lee Kristine M. Kim Eugene C. Yi |
| author_facet | Yu Ri Seo Junghyeon Lee Han Suk Ryu EunHee G. Kim So Hyun Kim Jieun Jeong Hyeryeon Jung YeoJin Jung Han Byeol Kim Yeon Hui Jo Yeong Dong Kim Min-Sun Jin Yong Yook Lee Kristine M. Kim Eugene C. Yi |
| author_sort | Yu Ri Seo |
| collection | DOAJ |
| description | Abstract Oncogenic cell-surface membrane proteins contribute to the phenotypic and functional characteristics of cancer stem cells (CSCs). We employed a proximity-labeling proteomic approach to quantitatively analyze the cell-surface membrane proteins in close proximity to CD147 in CSCs. Furthermore, we compared CSCs to non-CSCs to identify CSC-specific cell-surface membrane proteins that are closely interact with CD147 and revealed that lateral interaction between CD147 and CD276 concealed within the lipid raft microdomain in CSCs, confers resistance to docetaxel, a commonly used chemotherapy agent for various cancer types, including metastatic breast cancer. Moreover, we investigated the clinical relevance of CD147 and CD276 co-expression in HER2+ breast cancer (BC) and triple-negative breast cancer patients who underwent chemotherapy. We observed poor disease-free survival and Overall survival rates in patients of CD147 and CD276 (p = 0.04 and 0.08, respectively). Subsequent immunohistochemical analysis in independent cohorts of HER2+ BC support for the association between co-expression of CD147 and CD276 and a poor response to chemotherapy. Collectively, our study suggests that the lateral interaction between CD147 and its proximal partners, such as CD276, may serve as a poor prognostic factor in BC and a predictive marker for the critical phenotypic determinant of BC stemness. |
| first_indexed | 2024-03-10T22:01:21Z |
| format | Article |
| id | doaj.art-57b613c0a0214fe69b00b7717f0c2795 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-10T22:01:21Z |
| publishDate | 2023-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-57b613c0a0214fe69b00b7717f0c27952023-11-19T12:57:18ZengNature PortfolioScientific Reports2045-23222023-08-0113111410.1038/s41598-023-41416-7Lateral interactions between CD276 and CD147 are essential for stemness in breast cancer: a novel insight from proximal proteome analysisYu Ri Seo0Junghyeon Lee1Han Suk Ryu2EunHee G. Kim3So Hyun Kim4Jieun Jeong5Hyeryeon Jung6YeoJin Jung7Han Byeol Kim8Yeon Hui Jo9Yeong Dong Kim10Min-Sun Jin11Yong Yook Lee12Kristine M. Kim13Eugene C. Yi14Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology and College of Medicine or College of Pharmacy, Seoul National UniversityDepartment of Bio-Health Convergence, Kangwon National UniversityDepartment of Pathology, Seoul National University Hospital, College of Medicine, Seoul National UniversityDepartment of Systems Immunology, Division of Biomedical Convergence, College of Biomedical Science, Kangwon National UniversityDepartment of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology and College of Medicine or College of Pharmacy, Seoul National UniversityDepartment of Systems Immunology, Division of Biomedical Convergence, College of Biomedical Science, Kangwon National UniversityDepartment of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology and College of Medicine or College of Pharmacy, Seoul National UniversityDepartment of Systems Immunology, Division of Biomedical Convergence, College of Biomedical Science, Kangwon National UniversityDepartment of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology and College of Medicine or College of Pharmacy, Seoul National UniversityDepartment of Bio-Health Convergence, Kangwon National UniversityDepartment of Bio-Health Convergence, Kangwon National UniversityDepartment of Pathology, Bucheon St. Mary’s Hospital, College of Medicine, The Catholic University of KoreaDepartment of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology and College of Medicine or College of Pharmacy, Seoul National UniversityDepartment of Bio-Health Convergence, Kangwon National UniversityDepartment of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology and College of Medicine or College of Pharmacy, Seoul National UniversityAbstract Oncogenic cell-surface membrane proteins contribute to the phenotypic and functional characteristics of cancer stem cells (CSCs). We employed a proximity-labeling proteomic approach to quantitatively analyze the cell-surface membrane proteins in close proximity to CD147 in CSCs. Furthermore, we compared CSCs to non-CSCs to identify CSC-specific cell-surface membrane proteins that are closely interact with CD147 and revealed that lateral interaction between CD147 and CD276 concealed within the lipid raft microdomain in CSCs, confers resistance to docetaxel, a commonly used chemotherapy agent for various cancer types, including metastatic breast cancer. Moreover, we investigated the clinical relevance of CD147 and CD276 co-expression in HER2+ breast cancer (BC) and triple-negative breast cancer patients who underwent chemotherapy. We observed poor disease-free survival and Overall survival rates in patients of CD147 and CD276 (p = 0.04 and 0.08, respectively). Subsequent immunohistochemical analysis in independent cohorts of HER2+ BC support for the association between co-expression of CD147 and CD276 and a poor response to chemotherapy. Collectively, our study suggests that the lateral interaction between CD147 and its proximal partners, such as CD276, may serve as a poor prognostic factor in BC and a predictive marker for the critical phenotypic determinant of BC stemness.https://doi.org/10.1038/s41598-023-41416-7 |
| spellingShingle | Yu Ri Seo Junghyeon Lee Han Suk Ryu EunHee G. Kim So Hyun Kim Jieun Jeong Hyeryeon Jung YeoJin Jung Han Byeol Kim Yeon Hui Jo Yeong Dong Kim Min-Sun Jin Yong Yook Lee Kristine M. Kim Eugene C. Yi Lateral interactions between CD276 and CD147 are essential for stemness in breast cancer: a novel insight from proximal proteome analysis Scientific Reports |
| title | Lateral interactions between CD276 and CD147 are essential for stemness in breast cancer: a novel insight from proximal proteome analysis |
| title_full | Lateral interactions between CD276 and CD147 are essential for stemness in breast cancer: a novel insight from proximal proteome analysis |
| title_fullStr | Lateral interactions between CD276 and CD147 are essential for stemness in breast cancer: a novel insight from proximal proteome analysis |
| title_full_unstemmed | Lateral interactions between CD276 and CD147 are essential for stemness in breast cancer: a novel insight from proximal proteome analysis |
| title_short | Lateral interactions between CD276 and CD147 are essential for stemness in breast cancer: a novel insight from proximal proteome analysis |
| title_sort | lateral interactions between cd276 and cd147 are essential for stemness in breast cancer a novel insight from proximal proteome analysis |
| url | https://doi.org/10.1038/s41598-023-41416-7 |
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