Nanosilver–Silica Composite: Prolonged Antibacterial Effects and Bacterial Interaction Mechanisms for Wound Dressings
Infected superficial wounds were traditionally controlled by topical antibiotics until the emergence of antibiotic-resistant bacteria. Silver (Ag) is a kernel for alternative antibacterial agents to fight this resistance quandary. The present study demonstrates a method for immobilizing small-sized...
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MDPI AG
2017-09-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/7/9/261 |
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| author | Dina A. Mosselhy Henrika Granbohm Ulla Hynönen Yanling Ge Airi Palva Katrina Nordström Simo-Pekka Hannula |
| author_facet | Dina A. Mosselhy Henrika Granbohm Ulla Hynönen Yanling Ge Airi Palva Katrina Nordström Simo-Pekka Hannula |
| author_sort | Dina A. Mosselhy |
| collection | DOAJ |
| description | Infected superficial wounds were traditionally controlled by topical antibiotics until the emergence of antibiotic-resistant bacteria. Silver (Ag) is a kernel for alternative antibacterial agents to fight this resistance quandary. The present study demonstrates a method for immobilizing small-sized (~5 nm) silver nanoparticles on silica matrix to form a nanosilver–silica (Ag–SiO2) composite and shows the prolonged antibacterial effects of the composite in vitro. The composite exhibited a rapid initial Ag release after 24 h and a slower leaching after 48 and 72 h and was effective against both methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli (E. coli). Ultraviolet (UV)-irradiation was superior to filter-sterilization in retaining the antibacterial effects of the composite, through the higher remaining Ag concentration. A gauze, impregnated with the Ag–SiO2 composite, showed higher antibacterial effects against MRSA and E. coli than a commercial Ag-containing dressing, indicating a potential for the management and infection control of superficial wounds. Transmission and scanning transmission electron microscope analyses of the composite-treated MRSA revealed an interaction of the released silver ions with the bacterial cytoplasmic constituents, causing ultimately the loss of bacterial membranes. The present results indicate that the Ag–SiO2 composite, with prolonged antibacterial effects, is a promising candidate for wound dressing applications. |
| first_indexed | 2024-12-13T09:37:09Z |
| format | Article |
| id | doaj.art-57b6a21d94b34b2b830b60ebaba30011 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-12-13T09:37:09Z |
| publishDate | 2017-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-57b6a21d94b34b2b830b60ebaba300112022-12-21T23:52:18ZengMDPI AGNanomaterials2079-49912017-09-017926110.3390/nano7090261nano7090261Nanosilver–Silica Composite: Prolonged Antibacterial Effects and Bacterial Interaction Mechanisms for Wound DressingsDina A. Mosselhy0Henrika Granbohm1Ulla Hynönen2Yanling Ge3Airi Palva4Katrina Nordström5Simo-Pekka Hannula6Department of Chemistry and Materials Science, School of Chemical Engineering, Aalto University, 02150 Espoo, FinlandDepartment of Chemistry and Materials Science, School of Chemical Engineering, Aalto University, 02150 Espoo, FinlandDepartment of Veterinary Biosciences, Division of Veterinary Microbiology and Epidemiology, University of Helsinki, P.O. Box 66, 00014 Helsinki, FinlandDepartment of Chemistry and Materials Science, School of Chemical Engineering, Aalto University, 02150 Espoo, FinlandDepartment of Veterinary Biosciences, Division of Veterinary Microbiology and Epidemiology, University of Helsinki, P.O. Box 66, 00014 Helsinki, FinlandDepartment of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, 02150 Espoo, FinlandDepartment of Chemistry and Materials Science, School of Chemical Engineering, Aalto University, 02150 Espoo, FinlandInfected superficial wounds were traditionally controlled by topical antibiotics until the emergence of antibiotic-resistant bacteria. Silver (Ag) is a kernel for alternative antibacterial agents to fight this resistance quandary. The present study demonstrates a method for immobilizing small-sized (~5 nm) silver nanoparticles on silica matrix to form a nanosilver–silica (Ag–SiO2) composite and shows the prolonged antibacterial effects of the composite in vitro. The composite exhibited a rapid initial Ag release after 24 h and a slower leaching after 48 and 72 h and was effective against both methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli (E. coli). Ultraviolet (UV)-irradiation was superior to filter-sterilization in retaining the antibacterial effects of the composite, through the higher remaining Ag concentration. A gauze, impregnated with the Ag–SiO2 composite, showed higher antibacterial effects against MRSA and E. coli than a commercial Ag-containing dressing, indicating a potential for the management and infection control of superficial wounds. Transmission and scanning transmission electron microscope analyses of the composite-treated MRSA revealed an interaction of the released silver ions with the bacterial cytoplasmic constituents, causing ultimately the loss of bacterial membranes. The present results indicate that the Ag–SiO2 composite, with prolonged antibacterial effects, is a promising candidate for wound dressing applications.https://www.mdpi.com/2079-4991/7/9/261silver nanoparticlessilicacompositeprolonged silver leachingantibacterial effectsmechanisms of actionwound dressings |
| spellingShingle | Dina A. Mosselhy Henrika Granbohm Ulla Hynönen Yanling Ge Airi Palva Katrina Nordström Simo-Pekka Hannula Nanosilver–Silica Composite: Prolonged Antibacterial Effects and Bacterial Interaction Mechanisms for Wound Dressings Nanomaterials silver nanoparticles silica composite prolonged silver leaching antibacterial effects mechanisms of action wound dressings |
| title | Nanosilver–Silica Composite: Prolonged Antibacterial Effects and Bacterial Interaction Mechanisms for Wound Dressings |
| title_full | Nanosilver–Silica Composite: Prolonged Antibacterial Effects and Bacterial Interaction Mechanisms for Wound Dressings |
| title_fullStr | Nanosilver–Silica Composite: Prolonged Antibacterial Effects and Bacterial Interaction Mechanisms for Wound Dressings |
| title_full_unstemmed | Nanosilver–Silica Composite: Prolonged Antibacterial Effects and Bacterial Interaction Mechanisms for Wound Dressings |
| title_short | Nanosilver–Silica Composite: Prolonged Antibacterial Effects and Bacterial Interaction Mechanisms for Wound Dressings |
| title_sort | nanosilver silica composite prolonged antibacterial effects and bacterial interaction mechanisms for wound dressings |
| topic | silver nanoparticles silica composite prolonged silver leaching antibacterial effects mechanisms of action wound dressings |
| url | https://www.mdpi.com/2079-4991/7/9/261 |
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