Computational Investigation to Design Ofloxacin-Loaded Hybridized Nanocellulose/Lipid Nanogels for Accelerated Skin Repair
The pharmaceutical application of biomaterials has attained a great success. Rapid wound healing is an important goal for many researchers. Hence, this work deals with the development of nanocellulose crystals/lipid nanogels loaded with ofloxacin (OFX) to promote skin repair while inhibiting bacteri...
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MDPI AG
2022-09-01
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| author | Mona M. AbouSamra Nada M. El Hoffy Nahla A. El-Wakil Ghada E. A. Awad Rabab Kamel |
| author_facet | Mona M. AbouSamra Nada M. El Hoffy Nahla A. El-Wakil Ghada E. A. Awad Rabab Kamel |
| author_sort | Mona M. AbouSamra |
| collection | DOAJ |
| description | The pharmaceutical application of biomaterials has attained a great success. Rapid wound healing is an important goal for many researchers. Hence, this work deals with the development of nanocellulose crystals/lipid nanogels loaded with ofloxacin (OFX) to promote skin repair while inhibiting bacterial infection. Ofloxacin-loaded hybridized nanocellulose/lipid nanogels (OFX-HNCNs) were prepared and evaluated adopting a computational method based on regression analysis. The optimized nanogels (OFX-HNCN7) showed a spherical outline with an encapsulation efficiency (EE), particle size (PS) and zeta potential (ZP) values of 97.53 ± 1.56%, 200.2 ± 6.74 nm and −26.4 ± 0.50 mV, respectively, with an extended drug release profile. DSC examination of OFX-HNCN7 proved the amorphization of the encapsulated drug into the prepared OFX-HNCNs. Microbiological studies showed the prolonged inhibition of bacterial growth by OFX-HNCN7 compared to the free drug. The cytocompatibility of OFX-HNCN7 was proved by Sulforhodamine B assay. Tissue repair was evaluated using the epidermal scratch assay based on cell migration in human skin fibroblast cell line, and the results depicted that cell treated with OFX-HNCN7 showed a faster and more efficient healing compared to the control. In overall, the obtained findings emphasize the benefits of using the eco-friendly bioactive nanocellulose, hybridized with lipid, to prepare a nanocarrier for skin repair. |
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| id | doaj.art-57b73e93dc6440e3a9e4859037e621b9 |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-09T23:57:25Z |
| publishDate | 2022-09-01 |
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| record_format | Article |
| series | Gels |
| spelling | doaj.art-57b73e93dc6440e3a9e4859037e621b92023-11-23T16:22:40ZengMDPI AGGels2310-28612022-09-018959310.3390/gels8090593Computational Investigation to Design Ofloxacin-Loaded Hybridized Nanocellulose/Lipid Nanogels for Accelerated Skin RepairMona M. AbouSamra0Nada M. El Hoffy1Nahla A. El-Wakil2Ghada E. A. Awad3Rabab Kamel4Pharmaceutical Technology Department, National Research Centre, Giza 12622, EgyptFaculty of Pharmacy, Future University in Egypt, New Cairo 11835, EgyptCellulose and Paper Department, National Research Centre, Giza 12622, EgyptChemistry of Natural and Microbial Product Department, National Research Centre, Giza 12622, EgyptPharmaceutical Technology Department, National Research Centre, Giza 12622, EgyptThe pharmaceutical application of biomaterials has attained a great success. Rapid wound healing is an important goal for many researchers. Hence, this work deals with the development of nanocellulose crystals/lipid nanogels loaded with ofloxacin (OFX) to promote skin repair while inhibiting bacterial infection. Ofloxacin-loaded hybridized nanocellulose/lipid nanogels (OFX-HNCNs) were prepared and evaluated adopting a computational method based on regression analysis. The optimized nanogels (OFX-HNCN7) showed a spherical outline with an encapsulation efficiency (EE), particle size (PS) and zeta potential (ZP) values of 97.53 ± 1.56%, 200.2 ± 6.74 nm and −26.4 ± 0.50 mV, respectively, with an extended drug release profile. DSC examination of OFX-HNCN7 proved the amorphization of the encapsulated drug into the prepared OFX-HNCNs. Microbiological studies showed the prolonged inhibition of bacterial growth by OFX-HNCN7 compared to the free drug. The cytocompatibility of OFX-HNCN7 was proved by Sulforhodamine B assay. Tissue repair was evaluated using the epidermal scratch assay based on cell migration in human skin fibroblast cell line, and the results depicted that cell treated with OFX-HNCN7 showed a faster and more efficient healing compared to the control. In overall, the obtained findings emphasize the benefits of using the eco-friendly bioactive nanocellulose, hybridized with lipid, to prepare a nanocarrier for skin repair.https://www.mdpi.com/2310-2861/8/9/593lipidnanocellulosenanogelsfactorialregressionanti-bacterial assay |
| spellingShingle | Mona M. AbouSamra Nada M. El Hoffy Nahla A. El-Wakil Ghada E. A. Awad Rabab Kamel Computational Investigation to Design Ofloxacin-Loaded Hybridized Nanocellulose/Lipid Nanogels for Accelerated Skin Repair Gels lipid nanocellulose nanogels factorial regression anti-bacterial assay |
| title | Computational Investigation to Design Ofloxacin-Loaded Hybridized Nanocellulose/Lipid Nanogels for Accelerated Skin Repair |
| title_full | Computational Investigation to Design Ofloxacin-Loaded Hybridized Nanocellulose/Lipid Nanogels for Accelerated Skin Repair |
| title_fullStr | Computational Investigation to Design Ofloxacin-Loaded Hybridized Nanocellulose/Lipid Nanogels for Accelerated Skin Repair |
| title_full_unstemmed | Computational Investigation to Design Ofloxacin-Loaded Hybridized Nanocellulose/Lipid Nanogels for Accelerated Skin Repair |
| title_short | Computational Investigation to Design Ofloxacin-Loaded Hybridized Nanocellulose/Lipid Nanogels for Accelerated Skin Repair |
| title_sort | computational investigation to design ofloxacin loaded hybridized nanocellulose lipid nanogels for accelerated skin repair |
| topic | lipid nanocellulose nanogels factorial regression anti-bacterial assay |
| url | https://www.mdpi.com/2310-2861/8/9/593 |
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