MICROPROPAGATION OF CAIXETA Didymopanax morototoni (Aubl.) Dcne. et Planch.
<div class="Section1"><p>This study was carried out aiming to study the <em>in vitro </em>regeneration of caixeta (<em>Didymopanax morototoni</em> (Aubl.) Dcne. et Planch.). In order to establish a basic micropropagation protocol axillary buds and nodal...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | Portuguese |
| Published: |
Universidade Federal de Santa Maria
2009-09-01
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| Series: | Ciência Florestal |
| Subjects: | |
| Online Access: | http://cascavel.ufsm.br/revistas/ojs-2.2.2/index.php/cienciaflorestal/article/view/365 |
| Summary: | <div class="Section1"><p>This study was carried out aiming to study the <em>in vitro </em>regeneration of caixeta (<em>Didymopanax morototoni</em> (Aubl.) Dcne. et Planch.). In order to establish a basic micropropagation protocol axillary buds and nodal segments were desinfested with sodium hipochlorite 10 % and innoculated in two basal media: WPM (Lloyd & McCown, 1981) and MS (Murashige & Skoog, 1962) supplemented with BAP, TDZ, NAA, GA<sub>3</sub>, IBA and activated charcoal in defferent combinations and concentrations for each developmental step. The desinfestation method based on sodium hipochlorite during 10 minutes allowed the best results for the establishment of aseptic cultures in nodal segments. With these explants the regeneration rate and the shoot elongation were higher in the WPM medium containing 1,0 mg.l<sup>-1</sup> BAP. However after three subcultures it was observed a reduction in the regeneration rate. The rooting of regenerated shoots was observed in a WPM medium supplemented with 1,0 mg.l<sup>-1</sup> IBA. The acclimatization process allowed the plantlet survival in<em> ex vitro</em> conditions.</p></div> <br /> |
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| ISSN: | 0103-9954 1980-5098 |