Isolation and Characterization of Plant Growth Promoting Endophytic Bacteria from Desert Plants and Their Application as Bioinoculants for Sustainable Agriculture
Desert plants are able to survive under harsh environmental stresses inherent to arid and semiarid regions due to their association with bacterial endophytes. However, the identity, functions, and the factors that influence the association of bacterial endophytes with desert plants are poorly known....
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-09-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/10/9/1325 |
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| author | Muneera D. F. ALKahtani Amr Fouda Kotb A. Attia Fahad Al-Otaibi Ahmed M. Eid Emad El-Din Ewais Mohamed Hijri Marc St-Arnaud Saad El-Din Hassan Naeem Khan Yaser M. Hafez Khaled A. A. Abdelaal |
| author_facet | Muneera D. F. ALKahtani Amr Fouda Kotb A. Attia Fahad Al-Otaibi Ahmed M. Eid Emad El-Din Ewais Mohamed Hijri Marc St-Arnaud Saad El-Din Hassan Naeem Khan Yaser M. Hafez Khaled A. A. Abdelaal |
| author_sort | Muneera D. F. ALKahtani |
| collection | DOAJ |
| description | Desert plants are able to survive under harsh environmental stresses inherent to arid and semiarid regions due to their association with bacterial endophytes. However, the identity, functions, and the factors that influence the association of bacterial endophytes with desert plants are poorly known. These bacterial endophytes can be used as an untapped resource to favor plant growth and development in agro-ecosystems of arid regions. The present study is therefore focused on the isolation and identification of bacterial endophytes from two native medicinal plants (<i>Fagonia mollis</i> Delile and <i>Achillea fragrantissima</i> (Forssk) Sch. Bip.) growing spontaneously in the arid region of the South Sinai (Egypt), and characterization of their plant growth promoting (PGP) traits. Thirteen putative bacterial endophytes were isolated from the leaves of both plant species and characterized for their plant growth promoting abilities using molecular and biochemical approaches, as well as greenhouse trials. Selected endophytic bacterial strains were applied to maize plants (<i>Zea mays</i> L. var. Single cross Pioneer 30K08) to further evaluate their PGP abilities under greenhouse conditions. Isolated bacterial strains have variable plant growth promoting activities. Among these activities, isolated bacterial endophytes have the efficacy of phosphate solubilizing with clear zones ranging from 7.6 ± 0.3 to 9.6 ± 0.3 mm. Additionally, the obtained bacterial endophytes increased the productivity of indole acetic acid (IAA) in broth media from 10 to 60 µg·mL<sup>−1</sup> with increasing tryptophan concentration from 1 to 5 mg·mL<sup>−1</sup>. <i>Bacillus</i> and <i>Brevibacillus</i> strains were frequently isolated from the leaves of both plant species, and had significant positive effects on plant growth and shoot phosphorus (P) and nitrogen (N) contents. Results suggest that these endophytes are good candidates as plant growth promoting inoculants to help reduce chemical input in conventional agricultural practices and increase nutrient uptake and stress resilience in plant species. |
| first_indexed | 2024-03-10T16:34:49Z |
| format | Article |
| id | doaj.art-57bb2526cf0f4702973022edd7f69bde |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T16:34:49Z |
| publishDate | 2020-09-01 |
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| series | Agronomy |
| spelling | doaj.art-57bb2526cf0f4702973022edd7f69bde2023-11-20T12:33:47ZengMDPI AGAgronomy2073-43952020-09-01109132510.3390/agronomy10091325Isolation and Characterization of Plant Growth Promoting Endophytic Bacteria from Desert Plants and Their Application as Bioinoculants for Sustainable AgricultureMuneera D. F. ALKahtani0Amr Fouda1Kotb A. Attia2Fahad Al-Otaibi3Ahmed M. Eid4Emad El-Din Ewais5Mohamed Hijri6Marc St-Arnaud7Saad El-Din Hassan8Naeem Khan9Yaser M. Hafez10Khaled A. A. Abdelaal11Biology Department, College of Science, Princess Nourah Bint Abdulrahman University, Riyadh POX 102275-11675, Saudi ArabiaBotany and Microbiology Department, Faculty of Science, AL-Azhar University, Nasr City, Cairo 11884, EgyptCenter of Excellence in Biotechnology Research, King Saud University, Riyadh POX 2455-11451, Saudi ArabiaBiodiversity Centre, Institut de Recherche en Biologie Végétale, Université de Montréal and Jardin botanique de Montréal, Montréal, QC 22001, CanadaBotany and Microbiology Department, Faculty of Science, AL-Azhar University, Nasr City, Cairo 11884, EgyptBotany and Microbiology Department, Faculty of Science, AL-Azhar University, Nasr City, Cairo 11884, EgyptBiodiversity Centre, Institut de Recherche en Biologie Végétale, Université de Montréal and Jardin botanique de Montréal, Montréal, QC 22001, CanadaBiodiversity Centre, Institut de Recherche en Biologie Végétale, Université de Montréal and Jardin botanique de Montréal, Montréal, QC 22001, CanadaBotany and Microbiology Department, Faculty of Science, AL-Azhar University, Nasr City, Cairo 11884, EgyptDepartment of Agronomy, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611, USAExcellence Center (EPCRS), Plant Pathology and Biotechnology Laboratory, Faculty of Agriculture, Kafrelsheikh University, Kafr El Sheikh 33516, EgyptExcellence Center (EPCRS), Plant Pathology and Biotechnology Laboratory, Faculty of Agriculture, Kafrelsheikh University, Kafr El Sheikh 33516, EgyptDesert plants are able to survive under harsh environmental stresses inherent to arid and semiarid regions due to their association with bacterial endophytes. However, the identity, functions, and the factors that influence the association of bacterial endophytes with desert plants are poorly known. These bacterial endophytes can be used as an untapped resource to favor plant growth and development in agro-ecosystems of arid regions. The present study is therefore focused on the isolation and identification of bacterial endophytes from two native medicinal plants (<i>Fagonia mollis</i> Delile and <i>Achillea fragrantissima</i> (Forssk) Sch. Bip.) growing spontaneously in the arid region of the South Sinai (Egypt), and characterization of their plant growth promoting (PGP) traits. Thirteen putative bacterial endophytes were isolated from the leaves of both plant species and characterized for their plant growth promoting abilities using molecular and biochemical approaches, as well as greenhouse trials. Selected endophytic bacterial strains were applied to maize plants (<i>Zea mays</i> L. var. Single cross Pioneer 30K08) to further evaluate their PGP abilities under greenhouse conditions. Isolated bacterial strains have variable plant growth promoting activities. Among these activities, isolated bacterial endophytes have the efficacy of phosphate solubilizing with clear zones ranging from 7.6 ± 0.3 to 9.6 ± 0.3 mm. Additionally, the obtained bacterial endophytes increased the productivity of indole acetic acid (IAA) in broth media from 10 to 60 µg·mL<sup>−1</sup> with increasing tryptophan concentration from 1 to 5 mg·mL<sup>−1</sup>. <i>Bacillus</i> and <i>Brevibacillus</i> strains were frequently isolated from the leaves of both plant species, and had significant positive effects on plant growth and shoot phosphorus (P) and nitrogen (N) contents. Results suggest that these endophytes are good candidates as plant growth promoting inoculants to help reduce chemical input in conventional agricultural practices and increase nutrient uptake and stress resilience in plant species.https://www.mdpi.com/2073-4395/10/9/1325<i>Zea mays</i> L.environmental stressesendophytic bacteriaplant growth promoting ability |
| spellingShingle | Muneera D. F. ALKahtani Amr Fouda Kotb A. Attia Fahad Al-Otaibi Ahmed M. Eid Emad El-Din Ewais Mohamed Hijri Marc St-Arnaud Saad El-Din Hassan Naeem Khan Yaser M. Hafez Khaled A. A. Abdelaal Isolation and Characterization of Plant Growth Promoting Endophytic Bacteria from Desert Plants and Their Application as Bioinoculants for Sustainable Agriculture Agronomy <i>Zea mays</i> L. environmental stresses endophytic bacteria plant growth promoting ability |
| title | Isolation and Characterization of Plant Growth Promoting Endophytic Bacteria from Desert Plants and Their Application as Bioinoculants for Sustainable Agriculture |
| title_full | Isolation and Characterization of Plant Growth Promoting Endophytic Bacteria from Desert Plants and Their Application as Bioinoculants for Sustainable Agriculture |
| title_fullStr | Isolation and Characterization of Plant Growth Promoting Endophytic Bacteria from Desert Plants and Their Application as Bioinoculants for Sustainable Agriculture |
| title_full_unstemmed | Isolation and Characterization of Plant Growth Promoting Endophytic Bacteria from Desert Plants and Their Application as Bioinoculants for Sustainable Agriculture |
| title_short | Isolation and Characterization of Plant Growth Promoting Endophytic Bacteria from Desert Plants and Their Application as Bioinoculants for Sustainable Agriculture |
| title_sort | isolation and characterization of plant growth promoting endophytic bacteria from desert plants and their application as bioinoculants for sustainable agriculture |
| topic | <i>Zea mays</i> L. environmental stresses endophytic bacteria plant growth promoting ability |
| url | https://www.mdpi.com/2073-4395/10/9/1325 |
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